... the light chain of botulinum toxin D (List Biological Laboratories), and sense and antisense oligonucleotides were diluted in the vehicle solution (0.5 M KAc with 10 mM TrisHCl, pH 7.4, and 0.2% Fast Green to visualize the injection). ...
Snippet: … In some experiments, inhibitors were injected intracellularly into the sensory neuron 30 min before the start of an experiment, and were always compared with vehicle injections. EGTA (Calbiochem) and the light chain of botulinum toxin D (List Biological Laboratories) were diluted in the vehicle solution (0.5 M KAc with 10 mM TrisHCl, pH 7.4, and 0.2% Fast Green to visualize the injection).