Bacterial Toxin Research Citations

PBMC stimulation and flow cytometry

… Positive control samples were stimulated using Staphylococcal Enterotoxin B (SEB, List Biological Laboratories) at 1μg/mL. …

… Antibody titers of infected rats were measured by ELISA. Bordetella pertussis specific ELISA plates were coated with 50 µl of 108 B. pertussis grown as mentioned above for infection. For measurement of antibody titers to FHA (Enzo ALX-630-123-0100), PRN (GSK), PT (List Biological Laboratories #180), and ACT (Gift from Dr. Erik Hewlett), we coated plates with 50 µl of each antigen at 1µg/ml. …

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer

… Two doses of 500 ng pertussis toxin (List Biological Laboratories Inc., The Netherlands) were intravenously injected on day 0 (immunization day) and day 2. The EAE clinical score was evaluated every day, and onset of EAE was observed on days 7-9. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Pharmacological analysis of skeletal muscle contractions

Organoids at 8–9 weeks were imaged using phase contrast in a Cytation 5 reader (Biotek) with temperature and CO2 control (37 °C, 5% CO2). Media was changed 1 h prior to the experiment and a single site per well was used to establish a baseline of contractions immediately prior to compound addition. Botulinum neurotoxin type A (6.7 nM final concentration) (List Biological Laboratories, 130 A) and Tubocurarine (12.5 μM) (Tocris, 2820) were resuspended in water, per manufacturer’s instructions.

… After MOG immunization, those mice were administrated with each shot of 250 ng pertussis toxin (List Biological Laboratories, Campbell City, CA, USA) for a total of two shots on day 0 and 2 by the intraperitoneal injection, subsequently to develop EAE manifested …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Differentiation and stimulation of THP-1 monocytes

… 1µg/ml protective antigen (PA; 90 min; List Biological Laboratories …

• Product #171E – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

2.1. Mouse model and treatment

Vglut3DTR/+ mice were generated in our lab. We used the CRISPR/Cas9 approach to connect DTR to the C-terminus of Vglut3 via the P2A peptide. PCR and Southern blot methods were used to screen out the mice with the correct recombination. Lgr5CreERT2/+ (Stock #008875) and Rosa26-tdTomato (Stock #007914) mice were purchased from the Jackson Laboratory. The primers used for genotyping Lgr5creERT2/+, Rosa26-tdTomato and Vglut3DTR/+ mice are listed in Supplementary table 1. Tamoxifen (Sigma-Aldrich) was injected intraperitoneally at postnatal day (P)2 at 0.20 mg/g bodyweight. EdU (Click-iT EdU Imaging Kit; Invitrogen) was injected intraperitoneally from P3 to P5 once a day at a dose of 50 μg/g. DT (List Biological Laboratories) was injected intraperitoneally at 12.5 ng/g, 25 ng/g, or 37.5 ng/g at P2. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Immunization of the replication cohorts followed the protocol of Wagnon et al. [17].

Male C57B/6 J mice were immunized at 8–9 weeks of age with either GluN1168-187 (control peptide), GluN1359-378 (active peptide) or for our additional comparison with ovalbumin, each emulsified in an equal volume of CFA (as described above). Antigens (200 µg) were equally distributed over 4 subcutaneous injections, 2 at shoulders and 2 at hind limbs. In addition, mice received 2 intraperitoneal injections of 200 ng of Bordetella pertussis toxin (#180, List Biological Laboratories) in PBS, immediately after and 48 h after immunization.

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer

Animals and regents

Four- to five-week-old female C57BL/6 mice were obtained from the Experimental Animal Center of Guangdong (Guangzhou, China). … Pertussis toxin (PTX) was purchased from List Biological Lab (Epsom, England). …

EAE induction and assessment

EAE was induced as previously described immediately after the fecal collection [27]. Briefly, mice immunized subcutaneously in the flanks of 200 μg MOG35–55 peptide emulsified in CFA including 500 μg mycobacterium tuberculosis H37RA on day 0 and day 7. Immediately thereafter and on day 2, the mice received an intraperitoneal injection of 300 ng PTX in 100 μl PBS. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Diphtheria toxin (DT) administration.

Experimental mice (DTR mice, 6-9 weeks old) received 2 123 intramuscular injections of DT from Sigma-Aldrich (St. Louis, MO) or List Biological Laboratories, Inc. (Campbell, CA) at 25 ng/g, spaced 2d apart …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae