Bacterial Toxin Research Citations

… EAE was induced with 50 µg myelin oligodendrocyte glycoprotein (MOG35-55; Synpeptide, Shanghai, China) emulsified with 1 mg/ml complete Freund’s adjuvant (CFA; Sigma-Aldrich, Oakville, Ontario, Canada), and 200 or 400 ng of pertussis toxin (PTX; lot #180235A1A, CHO cell assay 0.03 ng/ml; List Biological Laboratories, Campbell, CA), as previously described (Segal et al., 2020) …

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer

Pertussis Toxin (PT) was purchased from List Biological Laboratories (Campbell, CA USA)

Induction of EAE. EAE was induced as described (17). In brief, acclimatized, 9-week-old, female C57BL/6J mice were immunized subcutaneously in the flank with 100 μg MOG emulsified 1:1 in IFA supplemented with 4 μg/mL of M. tuberculosis (H37Ra; Difco Laboratories). Animals received 200 ng by i.p. injection of PT on days 0 and 2 postimmunization. R-EAE was induced in acclimatized 9-week-old female SJL/J mice by immunization subcutaneously with 100 μL (50 μg) of PLP emulsified 1:1 in IFA containing 4 μg/mL of M. tuberculosis. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Pertussis toxin was from List Biologicals.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Experimental autoimmune encephalomyelitis

EAE was induced in 8–12 weeks old female C57BL/6J WT and ACKR1–/- C57BL/6J mice as described before [59, 60]. Pertussis toxin (List Biological Laboratories, Campbell, US) (300 ng in 100 μl PBS/mouse) was injected intraperitoneally (i.p.) on day 0 (immunization day) and day 2 post-immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… After a location was found from which a compound movement resembling the p-Synergy could be evoked with low currents, the stimulating pipette was removed from the brain, cleaned with deionized water, and filled with 1% cholera toxin (CT); List Biological Laboratories …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Animal studies and treatments

… Mice were treated with 0.5µg DT or CRM197 (List Biological) i.p. on day 0 only or on days 0, 4, 7, and 10. For in vivo competition assays, mice were treated on day 0 with 0.125-0.5µg DT and/or CRM197. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae
• Product #149A – Diphtheria Toxin CRM197, Mutant

… Anthrax Lethal Factor (LF-A) (#171E) and Anthrax Protective Antigen (PA) (#169L) were obtained from List Biological Laboratory (California, USA). …

• Product #171E – Anthrax Protective Antigen (PA), Recombinant from B. anthracis
• Product #169L – Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence

… On the same day as immunization and 2 days later, mice were also injected IP with 300 ng of pertussis toxin (List Biological Labs, Campbell, California, USA). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction and assessment of EAE

Active EAE was induced as previously described (38). EAE induction was performed with age- and sex-matched mice. Briefly, mice were injected subcutaneously with the MOG35–55 peptide (200 μg) in Complete Freund’s Adjuvant (CFA) containing heat-killed Mycobacterium tuberculosis (5 mg/ml; H37Ra strain, BD Diagnostics). On the day of immunization and 48 hours later, mice were injected intraperitoneally with Pertussis toxin (200 ng per mouse; List Biological Laboratories). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Microinjection of CTB Into the LR3 and LR8 on the Opposite Side of Hindlimb

Six rats were used for the injection of CTB into the LR3 and LR8 on the opposite sides of hindlimbs. Considering the application of
the CTB on the peripheral site only produced neural labeling on the same side of injection, bilateral injections were performed in
this study. This is also in order to reduce the number of animals used. Under the anesthesia with 2% isoflurane, 2 µl of 1% CTB
solution (List Biological Labs, Inc., Campbell, CA, United States) was injected subcutaneously and muscularly with Hamilton
micro-syringe (Hamilton Company, Reno, NV, United States) into the right side of LR3 and the left side of LR8, respectively. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt