Bacterial Toxin Research Citations

… Then, adherent macrophages underwent further incubation in serum-free RPMI1640 at 37°C for 2h and subsequent treatment with LPS (List Biological Laboratories, Escherichia coli 055:B5), …

• Product #203A – LPS from Escherichia coli O55:B5

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Induction of myelin-reactive Th17 cells ex vivo and the adoptive transfer EAE model

… To induce EAE by adoptive transfer, CD4+ T cells were isolated from the MOG35-55-specific Th17 cell culture with the CD4+ T Cell Isolation Kit 5 days after stimulation. CD4+YFP+ T cells further purified on a FACSAriaTM III were adoptively transferred into Tcrb−/− mice via tail vein injection (5 × 106 cells/injection). One day after transfer, recipient mice were immunized with MOG35-55 peptide in CFA and then given an intraperitoneal injection of pertussis toxin (PTX, 500 ng/injection) (List Biological Laboratories, Campbell, CA, USA)12. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE induction

Male MeCP2 WT and MUT mice, 9 weeks old, were anesthetized via i.p. with a mixture of xylazine and ketamine (16 mg/kg and 80 mg/kg respectively) and immunized subcutaneously at the right and left flanks with 200 μl of an emulsion containing 200 μg of myelin oligodendrocyte glycoprotein peptide (MOG35–55, NH2-MEVGWYRSPFSRVVHLYRNGK-COOH; synthesized at the Johns Hopkins University Synthesis & Sequencing Core Facility, Baltimore, MD, USA). The peptide was dissolved in sterile water at 2 mg/ml, mixed at a 1:1 ratio with complete Freund’s adjuvant (CFA, Sigma-Aldrich Co., St. Louis, MO, USA), supplemented with 4 mg/ml of Mycobacterium tuberculosis. Pertussis toxin (200 ng List Labs, USA) was dissolved in 100 μl of phosphate-buffered saline (PBS) and injected i.p. the same day of the immunization and 48 h later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… For immunofluorescence staining, tissue sections were stained with camelid anti-Stx1 and Stx2 (List Biological Laboratories, Campbell, CA, USA) followed by goat anti-llama:FITC (Invitrogen). …

Immunization and challenge.

Mice were immunized weekly for 6 weeks by oral gavage with 5mg ground blanched peanut (Western Mixers Produce & Nuts) and 10µg cholera toxin (CT) (List Biologicals) in 200µl of 0.2M sodium bicarbonate buffer per mouse …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… Experimental procedures Materials … LPS derived from Salmonella minnesota R595 (Re) was purchased from List Biological Laboratories, Inc. (Campbell, CA). …

• Product #304 – LPS from Salmonella minnesota R595 (Re)

… PA63 was prepared from PA83 using furin79 or purchased from List Biological Laboratories, Inc. (Campbell, CA, USA) in the purified form. …

• Product #171E – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Experimental procedures

Materials

… PTX was from List Biological Laboratories; …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Reagents

Highly purified rough LPS from E. coli K12 strain LCD25 was from List Biological Labs. …

• Product #314 – LPS from Escherichia coli K12 strain LCD25