Bacterial Toxin Research Citations

ELISPOT assay

ELISPOT was performed to enumerate cholera toxin and LPS specific plasmablast present in the PBMC samples. 96-well ELISPOT assay filter plates (Millipore) were coated overnight at 4°C with CTB (List Labs #104), …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Cell-rounding assay.

This assay was used to measure toxin activity, as C. difficile toxins cause cell rounding of tissue cultures, and was performed as described previously (43) with slight modifications. Briefly, Vero cells (monkey kidney epithelial cells) from ATCC were aliquoted in 24-well plates at a density of 103 cells/ml and incubated overnight at 37°C in a CO2 incubator. Cells were treated with serially diluted concentrations of ebselen (ranging from 125 ng/ml to 128 μg/ml) for 30 min, followed by the addition of TcdB (List Biologicals Laboratories, Inc.) to a final concentration of 20 ng/ml. The plates were incubated at 37°C in a CO2 incubator for 2 h and then observed with phase-contrast microscope (Nikon Diaphot 200/300 inverted microscope) with counting from 5 views per well.

• Product #155 – Toxin B from Clostridium difficile

… Control animals received subcutaneous injections lacking MOG33-55 peptide. At the time of immunization and 48?h later, mice received intraperitoneal injections with 500 ng pertussis toxin (List Biologicals, cat. no. 180)

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer

… Sections were the incubated with goat anti-CTB (List Biological Laboratories Cat #703, 1:10,000) …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

… Mice also received 250 ng of pertussis toxin (List Biological Laboratories) ip on days 0 and 2 postimmunization (pi). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… DT (List Biological Laboratories, 150) was dissolved in PBS, and 1µg (first dose) or 250ng (subsequent doses) was injected intraperitoneally for each mouse …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Top-down lipopolysaccharide structural analysis using multistage MS (MS/MS and MS3)

To determine the structures and validate the purity of different rough-type LPS preparations, top-down LPS structural elucidation was performed as described previously (27). Highly purified LPS of Bordetella pertussis 165 strain and Escherichia coli J5 strain were obtained from List Biological Laboratories, Inc. (Campbell, CA). LPS samples were dissolved at an approximate concentration of 20 μg ml−1 in 2-propanol/water (50:50 [vol/vol]). …

• Product #301 – LPS from Escherichia coli J5 (Rc)

Rocky Hill, NJ). Bone marrowderived macrophages (BMDMs) were washed twice with D-PBS (Wisent) to remove nonadherent cells and cultured in fresh IMDM 1 10% FBS alone or containing 1000 ng/ml LPS (List Biological Laboratories, Campbell, CA) or 40 mM hemin (Sigma-Aldrich, St. Louis, MO). A

… 250 ng of pertussis toxin (List Biological Laboratories) was administered ip on the day of immunization and again after 48 h. The disease symptoms in mice were observed and scored daily according to the following scoring criteria …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE induction and activity

Eight to twelve weeks-old mice were injected subcutaneously with 100mL of an emulsion containing 200mgofMycobacterium tuber-culosisH37Ra (Cat# DF3114-33-8, Difco) and 200mg of MOG35–55(Cat# MOG3555-P2, Genemed Synthesis) distributed over threesites on the flank. On day 0 and 2 after immunization, 200 ng pertussis toxin (Cat# 180, List Biological Laboratories) were adminis-tered intraperitoneally (i.p.). …

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer