Bacterial Toxin Research Citations

Study Design

This single-center, randomized, double-blind, placebo-controlled, full-factorial single-dose study was conducted between December 2016 and December 2017 in subjects in whom pyrexia was induced by intravenous (IV) administration of a reference standard endotoxin (RSE; ClinicalTrials.gov identifier: NCT02761980). In this standardized model, fever was induced by endotoxin (gram-negative bacterial lipopolysaccharide) derived from Escherichia coli O113, which was developed as a national biological reference standard in 1976 by the National Institute of Allergy and Infectious Diseases and the US Food and Drug Administration.17 The RSE used in this study was a new lot supplied by List Biological Laboratories, Inc. (Campbell, California). …

• Product #433 – LIST™ HPT™ from Escherichia coli O113

… The two tracers were fast blue (FB, Sigma-Aldrich, Gillingham, UK), and cholera-toxin b (CTB, List Biological Laboratories Inc, CA; 1% solution in 0.05M tris) …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

2.2. Experimental Design

… In the sensitization phase (day 0–28), mice in the PBS group were administered 10 µg of cholera toxin as an adjuvant (CT; List Biological Laboratories, Campbell, CA, USA) in 0.5 mL PBS via oral gavage. Mice in the other experimental groups were administered 20 mg whey together with 10 μg CT/0.5 mL PBS. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

• Product #152C – Toxin A from Clostridium difficile
• Product #155 – Toxin B from Clostridium difficile

… Pertussis toxin (PTX, 200 ng, List Biological Laboratories) was injected intravenously immediately and 2 days after immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Experimental Autoimmune Encephalomyelitis

For MOG35–55 induced EAE, age (8–12 weeks old) and gender matched mice were subcutaneously immunized (s.c.) with 50 μg of MOG35–55 peptide (MEVGWYRSPFSR VVHLYRNGK, AnaSpec) and 500 μg of M. tuberculosis (Difco) emulsified in IFA (Difco). In addition, the mice were administrated 200 ng of Pertussis Toxin (List Biological Laboratories) intraperitoneally (i.p.) on days 0 and 2, respectively. For adoptive transferred EAE, 2D2 CD4+ T cells were differentiated under pathogenic Th17 differentiation conditions for 4 days and re-stimulated in fresh medium in the presence of plate-bound anti-CD3 (2 µg/ml) and anti-CD28 (2 µg/ml) antibodies for 2 days. Live cells were purified as described. Approximately 1 × 106 purified cells were i.v. injected into Rag1−/− mice. Approximately 200 ng of Pertussis toxin (List Biological) was injected intra-peritoneal (i.p.) on days 0 and 2 after T cell transfer. For induced SKI expression in adoptive T cell transfer induced EAE, tamoxifen (TMA group) or corn oil (Control group) were injected intraperitoneally at days 1 and 3 after EAE elicitation as illustrated. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Protective Antigen List Biological Labs #171E

• Product #171E – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Pertussis toxin, List Biological Laboratories, Cat# 181

EAE induction

To induce EAE, a 1:1 incomplete Freund’s adjuvant (IFA) to PBS emulsion was prepared with final concentrations of 2 mg/ml desiccated Mycobacterium tuberculosis (Difco 231141) (to make complete Freund’s adjuvant, CFA) and 0.5 mg/ml myelin oligodendrocyte (MOG) peptide 35-55 (Genscript RP10245). Three injections were administered s.c. in the back skin (100 μl per site) such that each mouse received 150 μg total MOG. At the time of MOG immunization and 1 d later, 200 ng pertussis toxin (List Biological 181) was injected i.p. …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

EAE induction in mice

… Eight-ten week-old C57BL/6 N female mice (Charles River Laboratories, Montreal, QC, Canada), housed under specific pathogen free conditions, 4/cage, on a 12:12 h light cycle were used in this study. EAE was induced by subcutaneous immunization with 100 μg myelin oligodendrocyte glycoprotein (MOG35–55) in emulsion of a 1:1 volume with complete Freund’s adjuvant (CFA, containing 4 mg/ml of heat killed Mycobacterium tuberculosis, Difco Laboratories, Detroit, MI, USA). The mice were also injected with intravenous Bordetella pertussis toxin (200 ng; PTX, List Biological Laboratories Inc., Campbell, California, USA) in 1X Phosphate Buffered Saline (PBS) at the time of, and two days following, immunization …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)