Bacterial Toxin Research Citations

Immunization and challenge.

Mice were immunized weekly for 6 weeks by oral gavage with 5mg ground blanched peanut (Western Mixers Produce & Nuts) and 10µg cholera toxin (CT) (List Biologicals) in 200µl of 0.2M sodium bicarbonate buffer per mouse …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… Experimental procedures Materials … LPS derived from Salmonella minnesota R595 (Re) was purchased from List Biological Laboratories, Inc. (Campbell, CA). …

• Product #304 – LPS from Salmonella minnesota R595 (Re)

… PA63 was prepared from PA83 using furin79 or purchased from List Biological Laboratories, Inc. (Campbell, CA, USA) in the purified form. …

• Product #171E – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Experimental procedures

Materials

… PTX was from List Biological Laboratories; …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Reagents

Highly purified rough LPS from E. coli K12 strain LCD25 was from List Biological Labs. …

• Product #314 – LPS from Escherichia coli K12 strain LCD25

… inserted with a tungsten wire was filled with 0.5-2.0 µl of the mixture of A3V-RSV-mCherry and 0.5% CTB (List Labs, Campbell, CA, USA) CTB (#703, Lot#7032H, List Labs; RRID: AB_10013220), …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt
• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

… Cholera toxin (CT) was used as a positive control adjuvant [33], [34], [35] from List Biological Laboratories (Campbell, CA, USA). …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… After immunization, the mice were i.p. injected with 200 ng of pertussis toxin (List Biological Laboratories) on days 0 and 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE induction

Female C57BL/6 and SJL/J mice (7–9 weeks) were immunized with MOG35–55 and PLP139–151, respectively, to induce EAE as previously described [15]. … Immunized C57BL/6 and SJL/J mice were then i.p. administered with 200 ng pertussis toxin (List Biological Labs, Campbell, CA, USA) on day 0 and day 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae was purchased from List Biological Laboratories, INC. …

Cell culture

… The concentration of agonists or stimuli were used as below: For NLRP3 inflammasome activation: peritoneal macrophages were primed with LPS (100 ng/mL) for 3 h followed by stimulation with 5 mM ATP (1 h); 10 μM Nigericin (1 h); 200 μg/mL MSU (6 h). For non-canonical NLRP3 inflammasome activation, macrophages were primed for 4 h with Pam3CSK4 (100 ng/mL), then treated with LPS (2 μg/mL) combined with CTB (5 μg/mL) (CTB + LPS), or LPS (2 μg/mL) transfected with FuGENE HD (0.25% v/v) for 16 h. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt