Bacterial Toxin Research Citations

2.1 Cell lines and reagents

… Enterotoxin Type B from Staphylococcus aureus (SEB, List Biological Laboratories …

2.2.1 Day-to-day profiling of T cell activation

The assay was run using cryopreserved human PBMCs from a single healthy donor and were allowed to recover for 24 hours before use. On day 0, we stained human PBMCs with cell proliferation tracing dye (excitation: 488 nm and emission: 530/30 nm) and washed 3 times before plating into a 96-well plate at 2 million/mL (50 μL/well). We treated the cells by adding 50 μL/well of 3 different T-cell modulators in the respective wells: CD3/CD28 Dynabeads, PHA, or SEB. We performed an 11-point, 1:2 serial titration with duplicate wells per dose for each selected treatment. The final top concentration of the treatment in the assay well was 1 million/mL CD3/CD28 Dynabeads, 10 μg/mL PHA, or 100 ng/mL SEB, respectively. …

… The MOG35 55 peptide was administered in the left paralumbar region. Immediately after the first injection and 48 h later, 200 ng of the pertussis toxin (List Biological Industries, San Diego, CA, USA) was intraperitoneally (i.p.) administered as previously described [3]. All the animals were examined daily and evaluated for clinical signs of EAE. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

PBMC stimulation and flow cytometry staining

Cells were stimulated at the concentration of 5 million per milliliter for 12 h in presence of 2 μg/ml GAG PTE peptides (AIDS Reagent Program, Division of AIDS, National Institute of Allergy and Infectious Diseases [NIAID], National Institutes of Health [NIH]: HIV-1 PTE Gag Peptide Pool from NIAID, Division of AIDS), 1 μg/ml of staphylococcal enterotoxins B (SEB; List Biological Laboratories) or medium (negative control). …

… Two tetanus toxins (TTxn, from List Biologicals; product code #190B, and from HuA) were used to assess mAb binding to native toxin …

• Product #190 – Tetanus Toxin from Clostridium tetani

Reagents

… Pertussis toxin (PTX) from Bordetella pertussis, was obtained from List Biological Laboratories, …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Purified Prn was purchased from List Biological Laboratories (Campbell, CA). …

• Product #187 – Pertactin from B. pertussis (69 kDa Protein)

… Sorted macrophages were further purified by 1-hour adhesion onto tissue culture plates before stimulation with LPS (ultrapure LPS; 10 ng/ mL; List Biological Laboratories Inc; NC9633766) …

Author did not specify which List Labs LPS product was utilized in their research.
List Labs provides the following LPS products: https://listlabs.com/product-information/lipopolysaccharides/

… Specific antibody responses were assessed via ELISA. Plates were coated with trivalent DTPw vaccine (1/200 dilution), Diphtheria toxoid (0.5 µg/mL, List Labs) or …

• Product #151 – Diphtheria Toxoid, from Corynebacterium diphtheriae

… The animals received 200 ng pertussis toxin (List Biological Laboratories) i.p. on days 0 and 2. Clinical signs of EAE were monitored daily. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… The animals received 200 ng pertussis toxin (List Biological Laboratories) i.p. on days 0 and 2. Clinical signs of EAE were monitored daily. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)