Bacterial Toxin Research Citations

EAE induction
Active EAE was induced by injection of 200 μg of myelin oligodendrocyte glycoprotein epitope MOG35-55 (#RP10245, Genscript) emulsified in complete Freund’s adjuvant (CFA) (#231131, BD). Mice received two subcutaneous (s.c.) injections into the flank and intraperitoneal injection (i.p.) of 200 ng pertussis toxin (PT) (List Biological Laboratories, #179A) the same day and two days after. …

• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Experimental autoimmune encephalomyelitis (EAE)
Mice were immunized 12 weeks after tamoxifen administration at the age of 15–16 weeks. MOG35-55/CFA was prepared by mixing 1 mg/ml MOG35-55 (GenScript) in PBS with Complete Freund’s Adjuvants (CFA, BD Biosciences) containing mycobacterium tuberculosis H37RA (BD Biosciences). 50 μg of the MOG35-55/CFA emulsion were injected subcutaneously into the tail base. Mice were also injected intraperitoneally with 150 ng pertussis toxin (List Biological Laboratories) in PBS at the day of immunization and two days later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Measurement of C. difficile TcdB concentration and sporulation capacity
TcdB concentration was quantified as previously reported [Citation37]. All 176 C. difficile isolates were cultured in brain heart infusion (BHI) broth at 37 ℃ for 48 h in an anaerobic chamber. TcdB concentration in BHI was measured and calculated using a formula according to a panel of purified TcdB standards (List Biological Laboratories, Campbell, CA, USA) with known concentrations ranging from 0.02 to 200 ng/μL. …

• Product #155 – Toxin B from Clostridium difficile

… For cell ablation, 1mg/ml stock solution of DTX (#150, List Biological Laboratories) was prepared with water, aliquoted and stored at 20°C until use. When needed, the DTX solution was diluted to 5ng/µl with sterile 0.9% saline and 50µg/kg was administered intraperitoneally (i.p.) daily for 5 consecutive days. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… Pertussis toxin List Labs Cat#180 …

Mice
Female 8-week-old C57BL/6 and C57BL/6 x DBA/2 F1 (B6D2F1) were purchased from Charles River Laboratories (#027 and #099) and maintained at Moderna in Cambridge, MA. …To run the EAE model 12-week-old C57BL/6 female mice were injected on day 0 at two sites on the rear flanks with a total of 100 μL of emulsion containing 100 μg of myelin oligodendrocyte glycoprotein peptide (MOG)35-55 peptide emulsified in CFA with 2.5 mg/mL Mycobacterium tuberculosis H37Ra. Mice were also injected with 100 μL of 6 μg/mL Pertussis toxin i.v. on days 0 and 2.

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer

… Lipopolysaccharide (LPS) was purchased from List Biological Laboratorie (San Jose, CA, USA; Cat.No.421). …

• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4

2.1.1. Experimental autoimmune encephalomyelitis (EAE) model

In brief, male and female C57BL/6 J mice were injected subcutaneously at two sites with an emulsion of 100 μl complete Freund’s adjuvant prepared by mixing incomplete Freund’s adjuvant (BD Biosciences) and Mycobacterium tuberculosis (BD Biosciences) and 100 μg MOG 35–55 (Bio-Synthesis Inc.). Mice received 200 ng bordetella pertussis (List Biological Laboratories, Inc) intraperitoneally at day 0 and day 2 after EAE induction. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Briefly, mice received two ip injections of pertussis toxin (PTX) (List Biological Laboratory, #181) dissolved in phosphate-buffered saline (PBS) (400 ng/mouse; day 0 and …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

Experimental animals

… The retrograde neuroanatomical tracer unconjugated CTB (List Biological Laboratories, Campbell, CA) was pressure-delivered into the caudate nucleus of one hemisphere and the putamen of the contralateral hemisphere in four monkeys (bilateral injections); whereas in one additional monkey, CTB was delivered into the GPi of one hemisphere and into the GPe of the contralateral hemisphere. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… Diphtheria Toxin from Corynebacterium Diphtheria (unnicked) List Biological Laboratories Inc Cat# 150 …

Tissue dissection, islet isolation and cell culture

… Islets and myeloid fractions obtained from each pancreas were combined by overnight culture in AggreWells (Stem Cells Technology) in RPMI-10% FCS-5mM glucose; recovered islets/myeloid cell aggregates were then cultured up to 10 days in the same medium in the presence or absence of 50 ng/ml of DT. Culture medium and DT were replaced every 48 hours.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae