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3951 citations found

Downregulation of DUOX1 function contributes to aging-related impairment of innate airway injury responses and accelerated senile emphysema

Schiffers, C;Lundblad, LKA;Hristova, M;Habibovic, A;Dustin, CM;Daphtary, N;Aliyeva, M;Seward, DJ;Janssen-Heininger, YMW;Wouters, EFM;Reynaert, NL;van der Vliet, A;

Product: Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

  • Studies with Primary Tracheal Basal Epithelial Cells

    Primary mouse tracheal basal epithelial cells (MTECs) were isolated from tracheas of either young (2 mo) or old (13–16 mo) wild-type (WT) C57BL/6J mice (Jackson Laboratories, Bar Harbor, ME) using overnight incubation with 0.1% protease 14 (Sigma-Aldrich, St. Louis, MO), as previously described (38, 39). Briefly, dissociated MTECs were propagated on a rat tail collagen I gel (BD Biosciences, San Jose, CA) in DMEM/F12 media (Invitrogen, Grand Island, NY) supplemented with 20 ng/mL cholera toxin (List Biological Laboratories, Campbell, CA), …

    Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Selection of a picomolar antibody that targets CXCR2-mediated neutrophil activation and alleviates EAE symptoms

Shi, X;Wan, Y;Wang, N;Xiang, J;Wang, T;Yang, X;Wang, J;Dong, X;Dong, L;Yan, L;Li, Y;Liu, L;Hou, S;Zhong, Z;Wilson, IA;Yang, B;Yang, G;Lerner, RA;

Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

  • EAE model studies

    The EAE model was induced in both wild type and hCXCR2 knock-in mice using synthetic myelin oligodendrocyte glycoprotein peptide (MOG35-55, MEVGWYRSPFS RVVHLYRNGK; GenScript). Briefly, mice were s.c. injected with an emulsion of 100 μg MOG35-55 peptide mixed with complete Freud’s adjuvant (IFA, #vac-ifa, InvivoGen) containing 400 μg Mycobacterium tuberculosis H37 Ra (#231141, Difco). A total of 400 ng pertussis toxin (#180, List Biological) were i.p. injected on the same day of MOG35-55 immunization and a second time at 48 h later. …

    Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer

Effect of dental malocclusion on cerebellar neuron activation via the dorsomedial part of the principal sensory trigeminal nucleus

Shi, M;Liu, X;Zhang, C;Zhang, H;Liu, Q;Wang, D;Liu, X;Li, J;Wang, M;

Product: Anti-Cholera Toxin B Subunit (Goat)

  • Light microscopy immunofluorescence histochemistry

    For CTb- and FG-injected rats, sections containing Vpdm plane were selected for immunofluorescence staining of neuronal nuclei (NeuN)/CTb/FG and NeuN/CTb/VGLUT1, respectively. The experimental procedures were as follows: (i) rabbit anti-VGLUT1 IgG (1:500; Synaptic System)/rabbit anti-FG IgG, mouse anti-NeuN IgG (1:1000; Millipore), and goat anti-CTb IgG (1:2000; List Biological) were used to incubate slices at room temperature overnight, …

    Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Product: Anthrax Protective Antigen (PA), Recombinant from B. anthracis

ADAM9 enhances Th17 cell differentiation and autoimmunity by activating TGF-?1

Umeda, M;Yoshida, N;Hisada, R;Burbano, C;Orite, SYK;Kono, M;Kyttaris, VC;Krishfield, S;Owen, CA;Tsokos, GC;

Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

Quinoa (Chenopodium quinoa Willd.) Seeds Increase Intestinal Protein Uptake

Ballegaard, AR;Larsen, JM;Rasmussen, PH;Untersmayr, E;Pilegaard, K;Bøgh, KL;

Product: Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

  • … White quinoa seeds (originating from Peru) were purchased from Procudan (Kolding, Denmark) and ground to flour. Capsaicin 97% pure (M2028, Sigma-Aldrich, St. Louis, MO, US) and CT (List Biological Laboratories, Campbell, CA, US) were included as control substances. …

    Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

AIM2 controls microglial inflammation to prevent experimental autoimmune encephalomyelitis

Ma, C;Li, S;Hu, Y;Ma, Y;Wu, Y;Wu, C;Liu, X;Wang, B;Hu, G;Zhou, J;Yang, S;

Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

  • Pertussis toxin (#180) was from List Biological Laboratories. …

    Induction and assessment of EAE

    To induce type A EAE, MOG35-55 peptide (300 µg per mouse) was emulsified with complete Freund’s adjuvant (200 µg M. tuberculosis H37Ra [Mtb] per mouse), and then subcutaneously injected in the flanks and neck of mice on day 0 for one immunization. Pertussis toxin (250 ng per mouse) was applied intravenously on days 0 and 2 after immunization. …

    Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer

A novel single-domain antibody multimer that potently neutralizes tetanus neurotoxin

de Smit Conceptualisation, H;Ackerschott, B;Tierney, R;Stickings, P;Harmsen Conceptualisation, M;

Product: Tetanus Toxin from Clostridium tetani

  • 2.2. Antigens and antibodies
    TeNT was obtained from List Biological Laboratories (USA).

    2.3. Phage display selection
    Llamas were injected intramuscularly simultaneously with both a ready to use tetanus toxoid vaccine intended for use in horses (1 ml, MSD Animal Health) and rTTC at day 0, 21 and 42. The rTTC (8 µg per injection) was emulsified with either Stimune adjuvant (Thermofisher Scientific, the Netherlands) for day 0 and 21 or with IMS1312 adjuvant (Seppic, France) for day 42. Serum samples were taken at day 0, 28 and 49. Heparinized blood samples were taken at day 28 and 49 for subsequent generation of phage display immune libraries in phagemid vector pRL144 [37], [41]. The libraries were rescued using VCSM13 helper phage. Phage display selections were performed by biopanning [42] with TeNT or rTTC presented by passive adsorption. Alternatively, plates were coated with cAb-TT2 or mAb TT10 [28], [39] in 50 mM NaHCO3 buffer (pH 9.6) for presentation of captured of TeNT. …

    2.4.1. Analysis of E. coli-produced VHHs
    Individual E. coli-produced VHHs clones were screened in an ELISA with coated TeNT or rTTC (at 1 µg/ml and 4 µg/ml respectively), and with cAb-TT2 or mAb TT10 (both were coated at 1 µg/ml in 50 mM NaHCO3 buffer (pH 9.6)) immobilised TeNT (at 1 µg/ml). …

    2.4.2. GT1b-TeNT inhibition ELISA
    A GT1b-TeNT inhibition ELISA was performed as reported [15]. Briefly, plates were coated with 10 µg/ml GT1b ganglioside in methanol at 100 µl/well by overnight incubation at RT. All subsequent incubations were performed in PBS containing 0.5% BSA and 0.05% Tween-20 for 1 h at RT. TeNT (1 µg/ml) was preincubated with VHHs, E. coli culture supernatants or mAbs in 100 µl/well in a plate for 1 h at RT. …

    Product #190 – Tetanus Toxin from Clostridium tetani

Glucan particles as a novel adjuvant for the induction of experimental autoimmune encephalomyelitis

Huizar, C;Ji, N;Reddick, R;Ostroff, G;Forsthuber, T;

Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

LRRC4 functions as a neuron-protective role in experimental autoimmune encephalomyelitis

Zhang, Y;Li, D;Zeng, Q;Feng, J;Fu, H;Luo, Z;Xiao, B;Yang, H;Wu, M;

Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

  • Mice and EAE induction

    … Wild-type littermate controls with same genetic background were used. All mice were maintained in specific pathogen-free conditions in the Laboratory Animal Department of Central South University. … EAE was induced by the use of myelin oligodendrocyte glycoprotein (MOG35–55, H-MEVGWYRSPFSRVVHLYRNGK-OH, GL Biochem) in 8-week-old female mice. Each mouse was immunized by subcutaneously injection with 200 μg of MOG35–55 emulsified in Complete Freund’s Adjuvant (CFA, Sigma) with 500 μg mycobacterium tuberculosis (strain H37RA, DIFCO). Mice were then intraperitoneally injected with 200 ng of pertussis toxin (List Biological Laboratories) at the time of immunization and 2 days after. …

    Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
    Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
    Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
    Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)