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3951 citations found

Antigen-encapsulating host extracellular vesicles derived from Salmonella-infected cells stimulate pathogen-specific Th1-type responses in vivo

Hui, WW;Emerson, LE;Clapp, B;Sheppe, AE;Sharma, J;Del Castillo, J;Ou, M;Maegawa, GHB;Hoffman, C;Larkin Iii, J;Pascual, DW;Edelmann, MJ;

Product: Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

  • In vivo murine experiments

    BALB/c 6- to 8-week old female mice (Charles River Laboratories, Inc., Worcester, MA) were used for the in vivo experiments. Mice were nasally vaccinated on days 0, 14, and 28 with 20 or 40 μg of exosomes derived from RAW264.7 cells infected with wild-type UK-1 S. Typhimurium (χ3761; exosomes were collected at two times points, 24–48 hpi, and pooled, n = 8 for each sample type). In some animals, we also included 2.5 μg of cholera toxin (CT; List Biologicals) or MPLA as an adjuvant (n = 8). …

    Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

STING Agonist Mitigates Experimental Autoimmune Encephalomyelitis by Stimulating Type I IFN-Dependent and -Independent Immune-Regulatory Pathways

Johnson, BM;Uchimura, T;Gallovic, MD;Thamilarasan, M;Chou, WC;Gibson, SA;Deng, M;Tam, JW;Batty, CJ;Williams, J;Matsushima, GK;Bachelder, EM;Ainslie, KM;Markovic-Plese, S;Ting, JP;

Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

Conditional Deletions of Hdc Confirm Roles of Histamine in Anaphylaxis and Circadian Activity but Not in Autoimmune Encephalomyelitis

Morin, F;Singh, N;Mdzomba, JB;Dumas, A;Pernet, V;Vallières, L;

Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

Macrophage migration inhibitory factor promotes the migration of dendritic cells through CD74 and the activation of the Src/PI3K/myosin II pathway

Ives, A;Le Roy, D;Théroude, C;Bernhagen, J;Roger, T;Calandra, T;

Product: ULTRA PURE LPS from Salmonella minnesota R595 (Re)

  • 2.2 Mice and cells

    ,,, Loosely adherent bone marrow derived dendritic cells (BMDCs) were collected after seven days. BMDCs were incubated 36 hours with 10 ng/mL Ultrapure Salmonella minnesota LPS (List Biologicals Laboratories, Campbell, CA) to generate activated DCs.

    Product #434 – ULTRA PURE LPS from Salmonella minnesota R595 (Re)

CNS Autoimmune Responses in BCMA-Deficient Mice Provide Insight for the Failure of Atacicept in MS

Kumar, G;Maria, Z;Kohli, U;Agasing, A;Quinn, JL;Ko, RM;Zamvil, SS;Axtell, RC;

Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

  • Western blot

    To detect PT production by Western blot, to a 1.0 mL aliquot of culture supernatant, ¼ volume of 100% TCA was added, mixed, and the sample was held on ice for 1 h. Samples were centrifuged at 16,000 × g for 30 min at 4 °C and the supernatant was removed. The TCA-precipitated protein pellet was resuspended in 120 µL 1 × NuPAGE SDS Sample Buffer (Invitrogen) in the presence of 1 × NuPAGE Reducing Agent (Invitrogen) and denatured at 90 °C for 10 min. A 10 µL sample (equal to 0.25 OD600 units of cell culture supernatant) was fractionated on NuPAGE 4 ~ 12% Bis–Tris Gel (Invitrogen) in NuPAGE MES SDS Running Buffer (Invitrogen), alongside SeeBlue Plus2 Pre-Stained Standard (Invitrogen) and purified PT containing subunits S1–S5 (List Biological Laboratories, Inc.), loaded in the amounts indicated in Fig. 6. Separated proteins were transferred to PVDF filter paper (Invitrogen) according to the manufacturer’s instructions. …

    Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
    Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
    Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
    Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Developing a Novel Multiplexed Immune Assay Platform to Screen Kinase Modulators of T Cell Activation

Liu, Z;Gomez-Donart, A;Weldon, C;Senutovitch, N;O’Rourke, J;

Product: Enterotoxin Type B from Staphylococcus aureus

  • 2.1 Cell lines and reagents

    Enterotoxin Type B from Staphylococcus aureus (SEB, List Biological Laboratories

    2.2.1 Day-to-day profiling of T cell activation

    The assay was run using cryopreserved human PBMCs from a single healthy donor and were allowed to recover for 24 hours before use. On day 0, we stained human PBMCs with cell proliferation tracing dye (excitation: 488 nm and emission: 530/30 nm) and washed 3 times before plating into a 96-well plate at 2 million/mL (50 μL/well). We treated the cells by adding 50 μL/well of 3 different T-cell modulators in the respective wells: CD3/CD28 Dynabeads, PHA, or SEB. We performed an 11-point, 1:2 serial titration with duplicate wells per dose for each selected treatment. The final top concentration of the treatment in the assay well was 1 million/mL CD3/CD28 Dynabeads, 10 μg/mL PHA, or 100 ng/mL SEB, respectively. …

    Product #122 – Enterotoxin Type B from Staphylococcus aureus

Suppression of neuroinflammation by an allosteric agonist and positive allosteric modulator of the ?7 nicotinic acetylcholine receptor GAT107

Mizrachi, T;Marsha, O;Brusin, K;Ben-David, Y;Thakur, GA;Vaknin-Dembinsky, A;Treinin, M;Brenner, T;

Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

T cell immune discriminants of HIV reservoir size in a pediatric cohort of perinatally infected individuals

Rinaldi, S;de Armas, L;Dominguez-Rodríguez, S;Pallikkuth, S;Dinh, V;Pan, L;G?rtner, K;Pahwa, R;Cotugno, N;Rojo, P;Nastouli, E;Klein, N;Foster, C;De Rossi, A;Giaquinto, C;Rossi, P;Palma, P;EPIICAL consortium, ;

Product: Enterotoxin Type B from Staphylococcus aureus

  • PBMC stimulation and flow cytometry staining

    Cells were stimulated at the concentration of 5 million per milliliter for 12 h in presence of 2 μg/ml GAG PTE peptides (AIDS Reagent Program, Division of AIDS, National Institute of Allergy and Infectious Diseases [NIAID], National Institutes of Health [NIH]: HIV-1 PTE Gag Peptide Pool from NIAID, Division of AIDS), 1 μg/ml of staphylococcal enterotoxins B (SEB; List Biological Laboratories) or medium (negative control). …

    Product #122 – Enterotoxin Type B from Staphylococcus aureus

Characterisation of tetanus monoclonal antibodies as a first step towards the development of an in vitro vaccine potency immunoassay

Riches-Duit, R;Hassall, L;Kogelman, A;Westdijk, J;Rajagopal, S;Davletov, B;Doran, C;Dobly, A;Francotte, A;Stickings, P;

Product: Tetanus Toxin from Clostridium tetani