Bacterial Toxin Research Citations

Cell Stimulation

RAW264.7 macrophages were stimulated with 100ng/ml E. coli O111:B4 [Sigma-Aldrich, L260], J5 [List Biological Laboratories, 301], and K12, D31m4 [List Biological Laboratories, 302] LPS for various durations to analyse the effect of LPCAT2 and RNF19B silencing on the expression …

• Product #301 – LPS from Escherichia coli J5 (Rc)
• Product #302 – LPS from Escherichia coli K12, D31m4 (Re)

4.1. Drugs and chemicals

… Pertussis toxin was purchased from List Biological Laboratories, Inc. (Campbell, CA, USA). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Materials

… chicken polyclonal IgY against TcdB (#754A) from List Biological Labs …

2.2. Probe conjugations

… The number of oligonucleotides that successfully modified onto the DNP was quantified using the Qubit® ssDNA Assay Kit (Invitrogen by Thermo Fisher Scientific, USA). BNP probes were fabricated as follow: the DNA-modified DNPs (10 nM, 0.2 mL) were reacted with excess GBP-tagged BoNT/A LC (0.3 μM, 0.2 mL) for 0.5 h at 37 °C. Then it was collected and resuspended with reaction buffer (50 mM HEPES, pH 7.4, 500 mM NaCl, 0.05% TWEEN20) for later usage. And the amount of BoNT/A LC conjugated onto the BNP was estimated using the SNAPtide (FITC/DABCYL, List Biological Laboratories, Campbell, CA).

• Product #521 – SNAPtide® Peptide Substrate (FITC/DABCYL) for C. botulinum Type A Neurotoxin

4.1.2. Mouse Model

EoP was induced in C57BL/6j mouse pups using a combination of hypoxia-ischemia (HI) and systemic inflammation, as described recently by our group [32], which is an adaptation of the model originally described by from Shen, Plane, and Deng [85]. At P5 HI was induced by permanent unilateral occlusion of the right common carotid artery under isoflurane anesthesia (5–10 min; 5% induction, 3% maintenance with flow O2: air 1:1). After a 75-min recovery period, pups were subjected to hypoxia (6% O2) during 35 min under temperature-controlled conditions (35.8–36.0 °C). Subsequently, animals received an i.p. injection of 1mg/kg LPS (List Biological Laboratories, Campbell, CA, USA), dissolved in sterile 0.9% NaCl. …

Author did not specify which List Labs LPS product was utilized in their research.
List Labs provides the following LPS products: https://listlabs.com/product-information/lipopolysaccharides/

2.1 EAE induction and drug delivery

… Pertussis toxin (List Biological labs Inc., Campbell, CA) was administered via intraperitoneal (ip) injection at day 0 (75 ng per mouse) and day 2 (200 ng per mouse) after immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Cell culture and gene expression analysis

Cell culture plates (48-well) were coated overnight with PN, PN Fr., or other indicated allergens at 50 µg/ml in carbonate-bicarbonate buffer (0.05M, pH 9.6, Millipore-Sigma) and washed once with PBS, before mDC or other cell types were added (2-5×105 cells/well) in StemSpan medium. The controls lipopolysaccharide (LPS, 100 ng/ml; Millipore-Sigma) and cholera toxin (CT, 1 µg/ml; List Biological Laboratories, Campbell, CA) were used in solution instead of coated. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

2.2. Experimental Autoimmune Uveitis (EAU) Induction

EAU was induced by subcutaneous injection of interphotoreceptor retinoid-binding protein peptide 1–20 (IRBP; [Homo sapiens] H2N-GPTHLFQPSLVLDMAKVLLD-OH, New England Peptide, Gardner, MA, USA) 500 μg per mouse emulsified in CFA containing 3.3 mg/mL of heat-killed Mycobacterium tuberculosis H37Ra (Difco, Franklin Lakes, NJ, USA) immediately followed by intraperitoneal application of pertussis toxin (PTx; List Biological Laboratories, Inc., Campbell, CA, USA) 1.2 μg, as previously reported [36].

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

STAR★Methods
Key resources table

Pertactin from B. Pertussis (69 kDa Protein) List Biological Laboratories CAT # 187

Luminex

A multiplexed Luminex assay was used to determine relative titer of antigen-specific isotypes, subclasses, and FcR binding, as previously described (Brown et al., 2017). For this assay, the following antigens were used: SARS-CoV-2 RBD (kindly provided by Aaron Schmidt), SARS-CoV-2 S (kindly provided by Eric Fischer), SARS-CoV-2 N (Aalto Bio Reagents). SARS-CoV-2 S1 (Sino Biological), SARS-CoV-2 S2 (Sino Biological), pertussis pertactin (List Reagents) and a mix of HA A/Michigan/45/2015 (H1N1), HA A/Singapore/INFIMH-16-0019/2016 (H3N2), B/Phuket/3073/2013 (Immunetech). …

• Product #187 – Pertactin from B. pertussis (69 kDa Protein)

… Following overnight incubation in primary antibody (goat anti-CTB; List Biological Science, Campbell, CA) and extensive washing, sections were incubated in secondary antibody …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)