Bacterial Toxin Research Citations

… Two days prior to the end of a 14-day survival period, cholera toxin B subunit (CTB, 3 800 µl/eye, 2 µg/µl, List Biological Laboratories, Inc., 103B) was injected intraocularly as an anterograde tracer to label axons regenerating through the optic nerve …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Materials

CT and CTA were purchased from List Biologicals (Campbell, CA); …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… Primary: goat anti-cholera toxin B subunit (diluted 1:2000) List Biological Labs, Inc Lot: 7032A9; …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

… Mice were injected intravenously with 100 µl BP toxin (List Biological Laboratories, Inc., Campbell, CA, USA) at 0.2 µg per mouse on days 3 and 5 post gavage (single gavage experiment) or days 7 and 9 post first gavage (double gavage experiment) …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Following baseline lavage, LPS (Endotoxin from E. coli Type O113 [Clinical Center Reference Endotoxin], List Biological Laboratories, Campbell, CA, USA) 40 EU per kg body weight in 10 mL of pre-warmed sterile saline (equivalent to 4 ng/kg) was instilled into the right middle lobe and 10 mL of pre-warmed sterile saline was given into one lingula segment of the contralateral side as control challenge …

• Product #433 – LIST™ HPT™ from Escherichia coli O113

… On days 16-18 of culture, the slices were treated with MCH and/or inhibitor in primary-GM and fixed. For this study, Gi/o protein inhibitor pertussis toxin (PTX; List Biological Laboratories Inc., Campbell, CA) and Akt inhibitor Akti1/2 were used …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Chromatogram binding assays

… Binding of 125I-labeled Erythrina cristagalli lectin (Sigma-Aldrich), and cholera toxin B-subunits (List Laboratories), to glycosphingolipids on thin-layer chromatograms was done as described19,39.

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Induction of monophasic or relapsing–remitting EAE

Monophasic EAE was induced in 8–10-week-old female C57BL/6 mice by subcutaneous injection of 300 µg of MOG35-55 peptide (Biomatik, USA, LLC; Sequence: MEVGWYRSPFSRVVHLYRNGK) emulsified in Complete Freund’s Adjuvant (CFA) containing 500 µg of heat-inactivated Mycobacterium tuberculosis (Difco). On Days 0 and 2, 500 ng pertussis toxin (List Biologicals) was administered intraperitoneally. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE induction, clinical evaluation and GK treatment

For EAE induction, female mice (10-12 weeks old) were immunized subcutaneously with 300μg MOG35-55 (Chinapeptides, China) emulsified in complete Freund’s adjuvant (CFA) (100μg/mouse, supplemented with 5mg/ml heat-inactive Mycobacterium tuberculosis H37RA) and injected intraperitoneally with 300ng pertussis toxin (List Biological Laboratories, USA) at the same time of immunization and again 48h after immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE model

… C57BL/6J mice were anesthetized by sevoflurane, and then subcutaneously injected with 100 μL MOG35-55 (3 mg/mL)/IFA (H37RA 4 mg/mL) (catalog: 231141, Difco Laboratories, USA) into the two sides of the upper part of the back on day 0. The mice were intraperitoneally (i.p.) injected with 50 μL of pertussis toxin (PTX) (0.5 μg/100 μL) (catalog: 180, List Biological Labs, Inc.). Forty-eight hours after the injection, the mice were injected with 50 μL of PTX for a second time. …

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer