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#301 (5 mg)
This product is discontinued.
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SF stimulation
SF were stimulated with human recombinant TNF (10 ng/ml; R&D Systems), human recombinant IL1β (1 ng/ml; R&D Systems), lipopolysaccaride (LPS) from Escherichia coli J5 (100 ng/ml; List Biological Laboratories), polyI-C (PIC) (10 μg/ml; InvivoGen), bacterial lipopeptide (bLP) palmitoyl-3-cysteine-serine-lysine-4 (300 ng/ml; InvivoGen) or human recombinant TGFβ (10 ng/ml; R&D Systems) for 24 h. ...
Materials and Methods
... Rc LPS - E. coli J5, List Biological Laboratories, 301; Re LPS - E. coli K12 D32m4, List Biological Laboratories, 302]. ...
... SF were stimulated with human recombinant TNF ... lipopolysaccaride (LPS) from Escherichia coli J5 (100 ng/ml; List Biological Laboratories), ...
Top-down lipopolysaccharide structural analysis using multistage MS (MS/MS and MS3)
To determine the structures and validate the purity of different rough-type LPS preparations, top-down LPS structural elucidation was performed as described previously (27). Highly purified LPS of Bordetella pertussis 165 strain and Escherichia coli J5 strain were obtained from List Biological Laboratories, Inc. (Campbell, CA). LPS samples were dissolved at an approximate concentration of 20 μg ml−1 in 2-propanol/water (50:50 [vol/vol]). ...
Cell Stimulation
RAW264.7 macrophages were stimulated with 100ng/ml E. coli O111:B4 [Sigma-Aldrich, L260], J5 [List Biological Laboratories, 301], and K12, D31m4 [List Biological Laboratories, 302] LPS for various durations to analyse the effect of LPCAT2 and RNF19B silencing on the expression ...
... collected by gentle pipetting, centrifuged at 300 3 g for 5 min at 25C, and resuspended in 3 ml of complete RPMI 1640 medium supplemented with 10 ng/ml recombinant murine GM-CSF, 1 mg/ml LPS (LPS from Escherichia coli J5(Rc); List Biological Laboratories ...
Materials:
... lyophilized LPS from E. coli J5 strain was purchased from both List Biological Laboratories, Inc ...
Western Blot Assays:
...Primary antibody was then added in 10 ml of 5% NFDM in TBST...and for PA, anti-protective antigen from B. anthracis, goat, 1:5,000, List Biological Laboratories, Campbell, CA) and incubated overnight at 4C. ...
... In addition, a smooth type LPS from E. coli O55:B5, and Rc type LPS from E. coli J5 (List Biological Laboratories, CA, USA), ...
... We used smooth-type LPS from E. coli O55: B5 (List Biological Laboratories, CA), Rc-type LPS from E. coli J5 (List Biological Laboratories), ...
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List Labs
540 Division Street
Campbell, California
List Biotherapeutics
9800 Crosspoint BIvd Suite 2011
Indianapolis, IN 46256, USA


