Bacterial Toxin Research Citations

… Clinical center reference endotoxin (GMP endotoxin prepared from E. coli O113 by List Biological Laboratories, Inc., Campbell, CA) was administered after informed consent to healthy subjects enrolled in an IRB-approved protocol (Pro00070829 at Duke University) …

• Product #9433 – GMP LPS Lipopolysaccharide List™ Hpt™ From Escherichia Coli Type O113

…CASP11 inflammasome activation was triggered by LPS (E. coli 0111:B4, 25ug/mL) transfection with FugeneHD, or LPS (E. coli 0111:B4, 25ug/mL) mixed with CTB (Cholera Toxin B Subunit, 20ug/mL)

Macrophage treatments:

…For NLRP3 inflammasome activation, ATP (5mM final concentration) or Nigericin (10uM final concentration) were prepared in Opti-MEM and added to macrophages in Opti-MEM. For CTB/LPS treatments, LPS (20-25ug/mL final concentration) and CTB (List Biological Laboratories #104; 20ug/mL final concentration) were mixed in Opti-MEM by pipetting up and down and allowed to incubate for 15-30min at room temperature.

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt
• Product #201 – LPS from Escherichia coli O111:B4

… Mice were also injected intraperitoneally with 400 ng of pertussis toxin (PTX) (List Biological Laboratories) on days 0 and 2 after immunization …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Reagents

… Pertussis toxin (PTX) was purchased from List biological labs (Campbell, CA). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Binding of the chimera to ganglioside

GM1 detected by ELISA. The ability of the chimera, CTB (LIST Biological Laboratories), or dsc19CfaE to bind to GM1 was determined by ELISA as
described previously (36). Samples were analyzed in triplicate.

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Tetramer, antibodies, and other reagents

… For antibody blocking experiments, BC-CML-bearing mice were injected with 200 μg/mouse αPD-1 (RMP1-14, BioLegend), 200 μg/mice αLAG3 (C9B7W;64 gift of Dario Vignali and Creg Workman), 200 μg/mice αTim-3 (RMT3-23, BioLegend), 500 μg/mice αTIGIT (10A7, Genentech) or respective murine or rat isotype controls (BioXCell), i.p. three times per week for 2 weeks starting from day 7 post BMT. Diphtheria toxin (DT) was from List Biological Laboratories. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Western Blotting

… Media concentrates and cell lysates were analyzed by Western blot (Figure S2) using antibodies against C. difficile toxin A or B (List Biological Labs, Inc., Campbell, CA, USA) followed by a goat anti-chicken horseradish peroxidase secondary antibody (Invitrogen).

Mouse model of -lactoglobulin food allergy: sensitization and probiotic administration.

Our model was adapted from the work of Li et al. (38). After an adaptation period, female BALB/cByJ mice
were either sensitized once a week for 5 weeks by intragastric administration of WP (15 mg/mouse;
Lacprodan 80; Arla, Lyon, France) and CT (10 µg/mouse; List Biological, Campbell, CA) suspended in
100 µl of PBS (sensitized group) or treated with 10 µg CT alone in PBS (nonsensitized group) (Fig. 1). …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

The introduction of Pertussis toxin

Ptx (List Biological Laboratories #180), was injected at 1.5μg/200μl. This was performed intra-venously 5 hours prior to live imaging by multi-photon microscopy. N = 3–5 animals per group, 33 total. Surgical set up takes about 15 minutes, and the animal is on the scope for about an hour—1 1/2 hrs resulting in 30–45 mins of usable data.

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer

Elisa

TcdA and TcdB toxins of the infected tree shrew stools were detected by ELISA method. The fresh fecal samples were collected after C. difficile infection for one week of all animals. Feces were suspended to 0.5 g/ml concentration by using PBS buffer (pH 7.4). The fecal suspensions were coated in 96-well plate at 4 °C overnight, and then washed with PBST (PBS pH 7.4 + 0.05% tween-20). The plate was blocked with 5% skim milk at 37 °C for 2 h. The polyclonal antibodies of TcdA and TcdB (List Biological Laboratories, Chicken IgY, 1: 1000 dilution) were used for first hybridization; then Goat anti-Chicken IgY Secondary Antibody, HRP (Invitrogen, 1: 5000 dilution) was used. ELISA-TMB Chromogenic Reagent kit (Sangon, China) was used to determine the absorbance at 450 nm. Pure toxin A and toxin B (List Biological Laboratories) were used as positive controls, and the standard curves were determined at 20 ng/ml, 2 ng/ml, 0.2 ng/ml, 0.02 ng/ml and 0.002 ng/ml both for TcdA and TcdB.

• Product #152C – Toxin A from Clostridium difficile
• Product #155 – Toxin B from Clostridium difficile