Bacterial Toxin Research Citations

EAE induction

Female C57BL/6 mice (Jackson Laboratories, 6–8 weeks of age) were immunized for chronic progressive EAE as described31 with 200 µg of MOG35-55 peptide (MEVGWYRSPFSRVVHLYRNGK; Stanford Pan Facility, Stanford, CA) in incomplete Freund adjuvant boosted with 4 mg/mL Mycobacterium tuberculosis (H37Ra, now complete Freund adjuvant [CFA]), whereas control or sham-immunized mice were immunized with CFA only. All mice received pertussis toxin (List Biologicals, Campbell, CA) intraperitoneally that day and 2 days later.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Surgery/microinjections

… For retrograde tracing, we used cholera toxin subunit b (CTb) (List Biological, Campbell, California; 0.1% in saline) (n = 4 mice). …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Neural tracing

In this study, a neural tracer, cholera toxin, subunit B (CTB), was injected into either the bladder body, bladder trigone, urethra or rectum in order to label sensory and autonomic neurons that innervate these regions. General anaesthesia was induced in animals using isoflurane (3% in oxygen for induction and 1.5%–2% for maintenance of anaesthesia). Pre‐emptive analgesia was induced by subcutaneous injection of 0.05 mg/kg buprenorphine (Temgesic; Reckitt Benckiser, Hull, UK). A midline incision was made in the lower abdomen and overlying organs displaced. CTB (0.3% w/v in sterile water; List Biolabs, Campbell, CA, USA) was microinjected using a Neuros 5‐µL syringe equipped with a 33‐G needle (65460‐03; Hamilton Co., Reno, NV, USA). …

Table 1. Primary antibodies

CTB Goat 1:10 000 703 List Biological Laboratories; Campbell, CA, USA

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt
• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

… Viable cells harvested on day 9 or 10 post stimulation were administered to nave A-J mice (3.04.0 107 cells-animal) intravenously that were primed with LPS on day -4 and day 0 (25 ?g-animal) (Basavalingappa et al., 2016; Hamada et al., 2004; Massilamany et al., 2016). Each animal also received pertussis toxin (PT, 100 ng; List Biologicals, Campbell, CA) on day 0 and 2 post-transfer (Agarwal et al., 2002; Fujimoto et al., 2006; Massilamany et al., 2011d) …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Pertussis toxin List Biologicals Cat# 179A …

• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE induction and clinical scoring

EAE was actively induced in 6- to 10-week old Sarm1 knockout mice and wild type littermates by subcutaneous injection of 200 μg of myelin oligodendrocyte glycoprotein peptide 35–55 (MOG35-55, Anaspec) emulsified in complete Freund’s adjuvant (CFA) containing 500 μg M. Tuberculosis H37 RA (Difco) and intraperitoneal injection of 300 ng pertussis toxin (List Biological Laboratories), followed by a second intraperitoneal injection of 300 ng pertussis toxin 2 days later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Mouse study design

Male C57Bl/6 mice or Saa3−/− mice on the C57Bl/6 background (described in7) between 2–4 months of age were injected with either LPS (intraperitoneal, 0.25, 2.5, or 25 µg, E Coli 0111:B4, List Biological Laboratory), silver nitrate (AgNO3, subcutaneous, 0.5 mL of a 1% solution, Sigma), or casein (subcutaneous, 0.5 mL of a 5% solution) (n = 3 mice/group). …

• Product #201 – LPS from Escherichia coli O111:B4

In Vitro Stimulation Assay.

… For the NFκB activity assay, Hox-derived macrophages were transduced with lentivirus carrying pHAGE NFκB-TA-LUC-UBC-GFP-W (a gift from Darrell Kotton (Boston University, Boston, MA); Addgene plasmid #49343) (69). Lipopolysaccharide (LPS) (Salmonella minnesota R595, List Biological Laboratories) (10 µg/mL) and 14,15-diHETrE (Cayman Chemical) (10 µM) were used to stimulate transfected macrophages for 6 h. Luciferase activity was measured using Nano-Glo luciferase assay as described by the manufacturer (Promega).

• Product #304 – LPS from Salmonella minnesota R595 (Re)

… For ablation of cDC1 studies, WT and XCR1DTR were injected i.p., with 25 ng diphtheria toxin (DT) (150, List Biological Lab) per gram of body weight on day 6 post tumour engraftment …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… were cultured in complete DMEM-F-12 medium (Gibco) with 5% horse serum (Gibco), 10 g-ml Insulin (Sigma-Aldrich), 20 ng-ml Epidermal Growth Factor (Sigma-Aldrich), 0.5 g-ml Hydrocortisone (Sigma-Aldrich,), and 100 ng-ml Cholera toxin (List Biological …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae