Bacterial Toxin Research Citations

… Pertussis fimbriae 2/3 and pertussis pertactin (69 kDa protein) were purchased from List Biological Labs (Campbell, CA) …

• Product #186 – Fimbriae 2/3 from B. pertussis
• Product #187 – Pertactin from B. pertussis (69 kDa Protein)

Materials and methods

Jak2V617F transgenic (TG) mice were generated as previously described [12]. CD11b-diphtheria toxin receptor (DTR) TG mice were purchased from The Jackson Laboratory (Bar Harbor, ME, USA) [13]. These mice were crossed to generate Jak2V617F/CD11b-DTR compound TG. To deplete monocytes in vivo, recipient mice transplanted with BMCs from CD11b-DTR TG mice or Jak2V617F/CD11b-DTR TG mice were administered 15 ng/g diphtheria toxin (DT) (List Biological Labs, Campbell, CA, USA) by intraperitoneal injection every 2 days for 8 weeks. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… Diphtheria toxoid (List Biological Laboratories, CA), …

• Product #151 – Diphtheria Toxoid, from Corynebacterium diphtheriae

Active-induced EAE Models (aEAE) and adoptive-transferred EAE (tEAE) models

… At 0 and 24 hours after immunization, mice were intraperitoneally injected with pertussis toxin (250 ng per mice, List Biological, Campbell, CA, USA).

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Key Resources Table
Diphtheria toxin (DT) List Biological Laboratories Cat# 150

In Vivo Cell Depletion

To ablate specific cells, CCR2-DTR, CCR2-DTR, Rag2-/-Il2rg-/-, ROSA26-iDTRMrp8-Cre, BDCA2-DTR, and non-transgenic littermate controls were injected i.p. with 10 ng/g body weight DT on Day -1, Day 0, and Day +2 pi (Espinosa et al., 2017, Swiecki et al., 2010), unless noted otherwise.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Lipopolysaccharides

We used the phenol–water method described by Westphal [25] to extract and purify the LPSs and cores from E. coli and Salmonella. The strains of E. coli that were used to obtain the LPSs and cores were as follows: … the core Ra came from S. minnesota (List Biological Laboratories, Campbell, C A, USA). The LPSs and cores were treated with DNase, RNase, and proteinase K. …

• Product #304 – LPS from Salmonella minnesota R595 (Re)

EAE

… Pertussis toxin (200 ng, ip)(List Biological Laboratories) was also injected at the same time as MOG(35-55) as well as 2 days later …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Anthrax Protective Antigen List Biologicals Ref: 771B…

Antigens, Peptides and Co-Stimulants

Purified genetically-detoxified Pertussis Toxin (PT; R9K, E129A; Limulus Amebocyte Lysate activity < 20 EU/mg) was purchased from LIST Biological laboratories Inc. (Campbell, CA, USA) and stored at 500 µg/mL at −20 °C.

Whole Blood Stimulation

… Different parallel stimulation conditions were performed, i.e., incubation in the presence of heat-inactivated PT (5 μg/mL), …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE induction and treatment

The EAE model was induced as previously described19. Mice were anaesthetized and subcutaneously immunized with 200 μl of 100 μg myelin oligodendrocyte glycoprotein (MOG35–55, Wuhan Haode Peptide, China) in incomplete Freund’s adjuvant (Sigma Aldrich, USA) containing 200 μg mycobacterium tuberculosis (strain H37Ra; Difco, USA) on day 0. Pertussis toxin (PTX; List Biologicals, USA) (200 ng) was administered intraperitoneally (i.p.) on day 0 and 2. EAE scores were assessed daily for clinical signs of EAE mice in a blinded fashion as previously described20. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)