Bacterial Toxin Research Citations

Reagents

Purified lipopolysaccharides (LPS) from Escherichia coli were obtained from List Biological Laboratory (Campbell, CA, USA) and dissolved in endotoxin-free water. …

Analysis of Inflammasome Activation

This was performed as described previously [33]. BMDMs were seeded at 2 × 106 cells/well in 6-well plates for immunoblot assay and 4 × 105 cells/well in 96-well plates for ELISA. BMDMs were primed with LPS for 4 h. To exclude the effect of sweroside on the LPS priming step, sweroside was added after washing out the LPS with phosphate-buffered saline. …

Author did not specify which List Labs LPS product was utilized in their research.
List Labs provides the following LPS products: https://listlabs.com/product-information/lipopolysaccharides/

… Intravenous LPS challenge. Intravenous LPS challenges were only performed on occasion 2 and 3. Subjects received 1 ng/kg (cohort 1) or 2 ng/kg (cohort 2) E. Coli-purified LPS (GMP-grade from Lot#94332B4, List Biological Laboratories Inc …

• Product #9433 – GMP LPS Lipopolysaccharide List™ Hpt™ From Escherichia Coli Type O113

Antibody quantification by ELISA

PA, LF and EF ELISAs were performed using commercially available antigens (List Biologicals, Campbell, CA) as previously described [21]. Cutaneous anthrax and regional control group sera were titered using 2-fold dilutions, beginning at 1:10. US control sera were titered at 10-fold dilutions, beginning at 1:10. …

• Product #171E – Anthrax Protective Antigen (PA), Recombinant from B. anthracis
• Prodcut #172 – Anthrax Lethal Factor (LF), Recombinant from B. anthracis
• Product #178A – Anthrax Edema Factor (EF), Recombinant from B. anthracis

… A microelectrode filled with a retrograde tracer, 1% CTb (List Biological Laboratories, Campbell, CA, USA) dissolved in 0.1 M phosphate buffer or 1% FG (Fluorochrome, Englewood, CO, USA) dissolved in saline was used. CTb or FG was electrophoretically (for 510 min with 2.0-A positive, 300 ms duration pulses at 2 Hz) injected into the OPC identified electrophysiologically as in the second experiment …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… Sections were incubated in a primary antibody against the inhibitory neuron transcription factor Pax2 (1:500 dilution, ThermoFisher #716000; RRID: AB2533990) or in antibodies against CTB (1:2000 dilution; List Biological Catalog #703, RRID: AB_10013220 …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Materials and Methods

A total of 40 female C57BL/6 mice, 10-week-old, were randomly divided into four groups, including induced control groups without EAE induction, induced EAE without training, and forced and voluntary training groups. All animals have started training 4 weeks before experimental autoimmune encephalitis- (EAE-) induction. The mice were intraperitoneally anesthetized with an injection of xylazine (5 mg/kg) and ketamine (50 mg/kg). Myelin oligodendrocyte glycoprotein peptide 35–55 (300 μg in saline; MOG 35–55; KJ Ross-Petersen ApS, Denmark) was emulsified in an equal volume of complete Freund’s adjuvant (CFA; Sigma, USA) containing 500 μg of heatkilled Mycobacterium tuberculosis. This emulsion was then injected subcutaneously at the base of the tail of each mouse. The mice also received intraperitoneal injection of pertussis toxin (300 ng in 10 μl PBS; List Biological Lab, USA) at the time of immunization and the same was repeated 48 hours later. To confirm EAE induction, animal clinical assessment of EAE was performed daily using a 15-point scoring system following immunization as reported previously by Giuliani et al. [13].

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Animal care and EAE immunization

… Mice received intraperitoneal (I.P.) injections with 400 ng (C57BL/6) or 200 ng (FoxP3EGFP) of Bordetella pertussis toxin (List Biological Laboratories) on day −21 and day −19 prior to transplant. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… and after rats resumed normal growth (required as evidence of recovery from surgery), they are briefly anesthetized to inject 2.5 ng TeNT (1 L in phosphatebuffered saline with 2% bovine serum albumin; produced by List Biological Inc …

• Product #190 – Tetanus Toxin from Clostridium tetani

… Prior to experimental manipulation, mice were anesthetized by intraperitoneal injection of ketamine (100 mg/kg) and xylazine hydrochloride (10 mg/kg). For intranasal or ocular immunization, 1 μg of TLA or TLA exo Ags with 2 μg cholera toxin (CT) (List Biological Laboratories, Campbell, California, USA) per head was suspended in 10 μl PBS and dropped 3 times at 2 week interval onto the surface of the cornea making a droplet or intranasally onto the nostrils using a micropipette. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… All dosing solutions were prepared in DMEM from stocks immediately prior to dosing for final dosing concentration of 40M or 10M and 0.0004% DMSO. Stocks were prepared of ultrapure lipopolysaccharide (LPS; 1 endotoxin unit (EU)/ng, E. coli 055:B5 Lot#4231A1 List Biological Laboratories, Campbell, CA) with sonication in nuclease free sterile water …

• Product #423 – ULTRA PURE LPS from Escherichia coli O55:B5