Bacterial Toxin Research Citations

EAE Induction and Scoring

… Two intraperitoneal injections of pertussis toxin, Bordatella pertussis, (List Biological Labs) were also administered to all mice on the day of induction and 48 hours thereafter. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Active EAE

… Immunization was administered subcutaneously (s.c.) on the back and tail base. Pertussis toxin (250 ng per mouse; List, Biological Laboratories, Campbell, CA, USA) in 200µl PBS was injected intraperitoneally twice, on the day of immunization (day 0) and again 2 days later (day 2). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Reagents

Lethal factor (LF) was purchased from List Biological Laboratories (#169, recombinant from B. anthracis)

• Product #169L – Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence

Protein expression and purification

Upon addition of double molar excess of trypsin inhibitor (Sigma Aldrich), PA7 was further purified by size exclusion chromatography using a Superdex 200 column (GE Healthcare). Lyophilized LF (List Biological Lab. Inc., Lot#1692A1B) were resuspended in water according to the manufacturer’s manual and mixed with PA7 in a molar ration of 10:1. …

• Product #169L – Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence

Chemicals for in vitro experiments

The following products were purchased from the indicated commercial sources: … Shiga-like toxin 2 (Stx, List Biological Laboratories, Inc.), …

• Product #162L – Shiga Toxin 2 from Escherichia coli

BMDM isolation and culture.

… TcdB toxin (PYRIN inducer, 0.05µg/mL, List Biological Laboratories) or flagellin (NLRC4 trigger, 1g/mL, Invivogen) were used to stimulate various canonical inflammasomes …

• Product #155 – Toxin B from Clostridium difficile

… Immunofluorescence 1×105 SCC-4 cells/well (biological triplicates) control and Clostridium difficile Toxin A – ToxA (List Biological Labs, Campbell, CA, USA) treated: 1, 2 and 4 µg/mL cultured on three-dimensional MatrigelTM for 24 h. …

• Product #152C – Toxin A from Clostridium difficile

Botulinum neurotoxin and its administration

Research-grade, purified, native BoNT serotype A1 (BoNT-A; 150 kDa) was purchased from List Biological Laboratories (Campbell, CA, USA). It was received as a lyophilized powder, which was reconstituted in 1 mg/mL phosphate buffer saline/bovine serum albumin (0.1%) to obtain a stock solution (0.1 mg/mL), which was aliquoted and kept frozen at −80 °C. Dilutions of aliquots were performed with 0.2% GPB to obtain the final concentrations (see below). …

… Adjuvanted formulations included 2µg of cholera toxin (List Biological Laboratories, 101B), 10µg of CTB (List Biological Laboratories, 103B), or …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… Ultrapure S. minnesota R595 LPS (List Biologicals) at a concentration of 1 g/ml was used for all cell culture LPS treatments unless otherwise noted. …

• Product #434 – ULTRA PURE LPS from Salmonella minnesota R595 (Re)