Bacterial Toxin Research Citations

… Samples were run in parallel for phenotypic analysis (above) and intracellular cytokine staining. 1-1.5 million PBMC suspensions were cultured at a final volume of 200µl of complete RPMI medium in the presence of Staphylococcus Aureus Enterotoxin B (SEB, List Biological Labs, CA) …

… Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae List Biological Laboratories Cat# 150 …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… LPS-neutralizing assays utilized smooth-type LPS from Escherichia. coli O55:B5 (List Biological Laboratories, Campbell, CA, USA) as an endotoxin. …

• Product #203A – LPS from Escherichia coli O55:B5

Chemicals and Reagents:

… Recombinant LF was from List Biological Laboratories (Campbell, California). …

Author did not specify which Lethal Factor (LF) was utilized; List Labs provides the following LF:

• Product #172 – Anthrax Lethal Factor (LF), Recombinant from B. anthracis
• Product #169L – Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence

Mouse Primary Corneal Epithelial Cells (MPCEC):

… Primary corneal epithelial cell cultures were established using a modification of the established explant culture method.^38,39 Briefly, eyes were enucleated, and the cornea was washed with Ca⁺⁺-free PBS (pH 7.2). Each cornea was cut in half and placed in a 35-mm dish (Fisher Scientific) with the epithelial side up. One and half milliliters of DMEM with 10% serum containing 40 g/mL gentamicin, 1% ITS, and 100 ng/mL cholera toxin (LIST Biological Laboratories, Inc., Campbell, CA, USA) was added and the tissue was cultured in a humidified incubator at 37C with 5% CO2. Culture medium was replaced every 2 days. Cells were passged using 0.25% trypsin (Fisher Scientific), and subculturing in DMEM with 3% serum containing 40 g/mL gentamicin, 1% ITS, and 100 ng/mL cholera toxin. 

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Cells and Reagents:

… Salmonella minnesota ultra pure lipopolysaccharide (LPS) was from List Biologicals Laboratories (Campbell, CA), …

• Product #434 – ULTRA PURE LPS from Salmonella minnesota R595 (Re)

… Cells were placed into six-well plates for stimulation with 10ngml1 Salmonella minnesota R595 (Re) ultra-pure LPS (List Biological) for different periods of time. …

• Product #434 – ULTRA PURE LPS from Salmonella minnesota R595 (Re)

EAE:

… Pertussis toxin (200 ng per mouse; no. 181, List Biological Laboratories) was administered intravenously 0 and 2 days after induction. Clinical evaluation was performed daily using a five-point scale: 0, no clinical signs; 1, limp tail; …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

EAE Induction:

Induction of EAE was performed as described earlier (33). Briefly, active EAE was induced by immunization with 200 µg MOG₃₅₅₅ peptide emulsified in complete Freund’s adjuvant (CFA, Sigma-Aldrich) containing 800 µg of heat-killed Mycobacterium tuberculosis (Difco Laboratories). The emulsion was administered s.c. as four 50µl injections into the flanks of each leg. In addition, 200 ng of pertussis toxin (List Biological Laboratories) dissolved in 200 µl PBS was injected i.p. on days 0 and 2 after immunization as described earlier (34). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Immediately after immunization and again 48 h later, the mice were received a tail vein injection of 200 ng of pertussis toxin (List Biological Laboratories, CA, USA) in 100 l of PBS (Zhang et al., 2017). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)