Bacterial Toxin Research Citations

… LPS (lipopolysaccharide; TLR4 agonist) List Biological Lab (San Jose, CA, USA), …

Author did not specify which List Labs LPS product was utilized in their research.
List Labs provides the following LPS products: https://listlabs.com/product-information/lipopolysaccharides/

… A total volume of 0.2 ml was injected sc into the flank at both right and left sides. On the same day and 48 h later, mice were injected ip with 150 ng in 0.2 ml of pertussis toxin (List Biological Laboratories)….

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Cholera toxin was obtained from List Biological Laboratories (Campbell, CA). …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Tissue clearing and nigral CTB tracing:

Lesion of the nigrostriatal pathway and VTA in Sprague-Dawley rats used for tissue clearing and light sheet microscopy or simultaneous rabies / CTB tracing received lesions was induced by unilateral injection of 6-hydroxydopamine with a volume of 3.0 L and 2.5 L at freebase concentration of 3.5 g/L to the following coordinates relative to bregma: A/P 4.4; M/L 1.2; D/V (from dura) 7.8; and A/P 4.0; M/L 0.8; D/V (from dura) 8.0, adjusted to flat head, respectively. Rats were grafted with 150,000 VM-patterned progenitors in two deposits to the striatum at a total volume of 2 L, at a concentration of 75,000 cells/L, at a rate of 1 L per minute and diffusion time of 2 min, to the following coordinates relative to bregma: A/P +0.5; M/L 2.6; D/V (from dura) 4.0/-3.5; adjusted to flat head. Animals grafted in the nigra received 75,000 cells in a total volume of 1 L to the following coordinates relative to bregma: A/P 5.2; M/L 2.3; D/V (from dura) 7.0; adjusted to flat head. For rabies / CTB tracing, animals first received two 500 nL nigral CTB (choleragenoid in low salt, List Biological Laboratories, 0.4% in PBS) injections at A/P 5.3; M/L 1.6, 2.6; D/V 7.2, 6.7 (from dura); adjusted to flat head relative to bregma, at a rate of 100 nL/min and a diffusion time of 3 minutes, followed by a total volume of 2 L G-rabies at a dilution of 5% of the stock injected at a rate of 0.5 L per minute and diffusion time of 2 min to the following coordinates relative to bregma: A/P +0.5; M/L 2.6; D/V (from dura) 4.0/-3.5; adjusted to flat head. Animals were perfused after 1 week of rabies / CTB tracing.

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Primary antibodies were used as follows:

… Goat anti-CTB (1:2500; List Biological Laboratories); …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Pertussis toxin (PT) List Biological Laboratories Cat#180

EAE

… Mice were also injected twice i.p. with 200 ng pertussis toxin (PT) (List Biological Laboratories) administered on the day of immunization and 48 h later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Serology by ELISA:

Serology was performed essentially as described [26]. Briefly, microtiter plates were coated with 2 µg/mL diphtheria toxoid (DT), tetanus toxoid, pertussis toxin (PT), filamentous hemagglutinin (FHA), or pertactin (Prn) (List Biological, Campbell, CA, USA) in 50 mM carbonate buffer (pH 9.6) at 4°C overnight. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

• Product #187 – Pertactin from B. pertussis (69 kDa Protein)
• Product #151 – Diphtheria Toxoid, from Corynebacterium diphtheriae

… these two groups were injected intraperitoneally with 250 ng pertussis toxin (PTx, List Biological Laboratories, Campbell, California, USA) in phosphate-buffered saline (PBS, Gibco, Grand Island, New York, USA) on 0 and 2 days after immunization …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Cholera toxin subinit B (CTB, 0.8% in 0.1 phosphate buffer; List Biological Laboratories Inc., Campbell, CA (List Biological, Cat# 703, RRID:AB_10013220, for details see antibody list in…

Author did not specify which CTB was utilized.  List Labs has provided Product #103B (Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae) and Product #104 (Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt).  The former has been discontinued.  The latter may be substituted for the former, if the researcher takes into account the difference in the buffer in which each product is provided and the amount of protein per vial.  When Product #103B was reconstituted with 200 l of water, it contained 1 mg of protein in 0.05 M Tris, 0.2 M NaCl, 0.001 M Na₂EDTA, 0.003 M NaN_3, pH 7.5.  When Product #104 is reconstituted with 0.25 ml water, it contains 0.5 mg of Cholera Toxin B Subunit (choleragenoid) in 0.01 M Sodium Phosphate at pH 7.5.

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

… In 5 animals, pFRG injections of the retrograde tracer, Cholera Toxin b (CTb, 1% in deionized water; List Biological, Campbell, CA) were unilaterally performed. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… goat anti-CTb, List Biological Laboratories, 1:50,000 dilution), in PBS with 0.25% Triton-X and 0.05% sodium azide. Tissue was then washed three times in PBS (30 s each) and incubated in biotinylated secondary (1:1000 dilution, …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)