Bacterial Toxin Research Citations

Chemicals

… PA63 protein was prepared from PA83 using furin (53) or purchased from List Biological Laboratories (Campbell, CA) in the purified form. …

• Product #174 – Anthrax Protective Antigen, Activated (PA 63) from B. anthracis

Induction and clinical scoring of EAE

Direct immunization against MOG35–55 in C57Bl/6J and TCR2D2 mice
Animals were immunized subcutaneously with 100 μg of MOG35–55 peptide (Genemed Synthesis, San Antonio, TX, USA) in complete Freund’s adjuvant (CFA) containing 400 μg Mycobacterium tuberculosis (Mt) H37Ra (Difco Laboratories, Detroit, MI, USA). Mice received 200 ng PT (List Biological, Campbell, CA, USA) by i.p. injection at the time of and 48 h post-immunization. Control mice were sham-immunized with phosphate-buffered saline in CFA and received the same PT dosage.

Direct immunization against PLP139–151 in SJL/J mice
SJL/J mice were injected with 100 μg PLP139–151 in 400 μg CFA subcutaneous and 2 × 50 ng PT i.p. on days 0 and 2. Control mice were sham-immunized with phosphate buffer saline in CFA and received the same PT dosage.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

2.2. Antigens

Vibrio cholerae toxin (CT; lot 10154B5A) and diphtheria toxoid (DPT; lot 1517B3) were from List Biological Labs (Campbell, CA, USA); …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae
• Product #151 – Diphtheria Toxoid, from Corynebacterium diphtheriae

… These inoculations were followed with pertussis toxin (PTx) (List Biologicals) …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Next, the cells were stimulated by adding 100 ng/ml LPS (ultrapure smooth LPS from E. coli O111:B4; List Biological Laboratories, Campbell, CA) for 1 h or 4 h. …

• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4

Pertussis toxin (200 ng, List Biological Laboratories) in PBS was administered i.v. on days 0 and 2. Mice were examined daily and scored for disease severity using the standard scale: 0, no clinical signs; 1, limp tail; 2, paraparesis (weakness, incomplete paralysis of one or two hind limbs); 3, paraplegia (complete paralysis of two hind limbs); 4, paraplegia with forelimb weakness or paralysis; 5, moribund or death. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… 1:1,000 for antiEF and antiLF sera, and antiPA antibody (List Labs, Campbell, California). …

First step c-Fos/CTb

Of the 28 animals used, 8 showed a suboptimal localization of the CTb and were excluded from this part of the study, therefore number of animal for each group changed to: Torpor n=4, Fasting n=4, Cold Exposure n=4, Control n=8. Following the blocking solution, all slices were incubated at room temperature overnight with the following primary antibodies: i) goat Anti-CTb (List Biological Laboratories); …

Second step neuronal typization

… 5.  Slices were then rinsed twice in PBS with 0,3% (v/v) Triton X-100 and incubated overnight at room temperature with: i) goat Anti-CTb (List Biological Laboratories; 1:10000); ii) rabbit Anti-c-Fos (Calbiochem; 1:10000).

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

E2 pellet implantation and induction of EAE

… Additionally, mice were administered 75 ng of pertussis toxin (Ptx, List Biologicals, Campbell, CA, USA) via an intraperitoneal (i.p.) injection on the day of immunization and 200 ng i.p. 2 days later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… The retrograde tracer, cholera toxin subunit (CTB; List Biological Labs, Campbell, CA, USA CTB serum (1:10000, List Biological Labs, Campbell, CA, USA) for 2 days. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt