Bacterial Toxin Research Citations

Indirect IFA:

… To test the membrane location of TgATPaseP-GC, the IMC was separated from the plasma membrane by treating extracellular parasites with -toxin from Clostridium septicum (20 nM, 2 h) (List Biological Laboratories), followed by fixation on BSA-coated coverslips and antibody staining. In both cases, the standard immunostaining procedure was performed subsequently.

… The vaccine contained a mixture of … and 1 µg cholera toxin as an adjuvant (List Biological Laboratories, Campbell, California, USA) suspended in phosphate-buffered saline. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Induction of EAE:

Mice from each group were immunized subcutaneously after a minimum of 30 days post TAM administration89 with 100 µg of MOG p35-55 peptide (2 mg/mL in PBS) emulsified in Complete Freunds Adjuvant containing 2 mg of heat-killed Mycobacterium tuberculosis (H37RA, Difco Laboratories). MOG p35-55 was synthesized by Genemed Synthetics (Genemed Synthetics Inc., San Antonio, TX, USA). Mice also received intraperitoneal injections with 100 ng – 250 ng of Pertussis toxin (List Biological Laboratories) on the day of immunization and 48 hours later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Diphtheria toxin (List Biological Laboratories) was administered by intraperitoneal injection of FAP-DTR mice (25 ng/g), twice a day, both in prophylactic (at day 7 and 5 before STIA) and in therapeutic regimes (at days 3 and 5 or at days 5 and 7 or at days 10 and 12 after STIA). …

• Product #150 – Diphtheria Toxin, Unnicked, fromCorynebacterium diphtheriae

… sensitized to EW by the oral administration of 5 mg of protein per mouse plus 10 µg of cholera toxin (CT, List Biologicals, Campbell, CA, USA) in PBS, during 3 consecutive days on the first week and once per week during the following 5 weeks, …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Antibody quantification:

Whole toxin A (List Biologicals, Campbell, CA) or recombinant rbdB was used to coat U-bottom high-binding 96-well ELISA plates (Greiner Bio-One, Frickenhausen, Germany). …

• Product #152C  – Toxin A from Clostridium difficile

Experimental Human Endotoxemia:

Purified LPS (continuous infusion study: Escherichia coli O:113, Lot no. 94332B4, List Biological Laboratories, Campbell, USA; bolus administration study: US Standard Reference Endotoxin Escherichia coli O:113, Pharmaceutical Development Section of the National Institutes of Health, Bethesda, USA) was supplied as a lyophilized powder and dissolved in normal saline 0.9% as described previously [23,24]. For the continuous infusion study (c-LPS), a total of 4 ng/kg LPS was administered as a intravenous loading bolus of 1 ng/kg body weight at T = 0, followed by an intravenous infusion of 1 ng/kg/h for a period of 3 h [24]. For the bolus administration study (b-LPS), LPS was administered, as described previously, as an intravenous bolus injection at a dose of 2 ng/kg body weight in 1 min at T = 0 [23].

All subjects received 1.5 L of 2.5% glucose/0.45% saline solution in the hour before initiation of LPS administration, followed by 150 mL/h during the first 6 h after LPS administration and 75 mL/h until the end of the experiment, according to our protocol [24].

• Product #9433 – GMP LPS Lipopolysaccharide List™ Hpt™ From Escherichia Coli Type O113

EAE induction and clinical assessment:

For immunization, incomplete Freunds adjuvant (IFA) was prepared by mixing paraffin oil (Sigma-Aldrich, St. Louis, USA; Cat # 18512) and mannide monooleate (Sigma-Aldrich; Cat # M8819) at a 9:1 ratio. Complete Freunds adjuvant (CFA) was subsequently obtained by adding 5mg/ml Mycobacterium tuberculosis H37 Ra (Difco Laboratories, Franklin Lakes, NJ, USA; Cat # 231141) to IFA. After emulsifying MP4 (Alexion Pharmaceuticals, Cheshire, CT, USA) in CFA, the mice were immunized subcutaneously into both sides of the flank with a total dose of 200g MP4. Additionally, an intraperitoneal injection of 200ng pertussis toxin (List Biological Laboratories, Hornby, ONT, Canada; Cat # 181) was given at the day of immunization and 48h later. …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

Induction and assessment of EAE:

For active EAE induction, age- and sex-matched mice were immunized s.c. with MOG_35-55 peptide (300 µg) mixed in CFA (Sigma-Aldrich) containing 5 mg/ml heat-killed Mycobacterium tuberculosis H37Ra (Difco). Pertussis toxin (200 ng, List Biological Laboratories) in PBS was administered i.v. on days 0 and 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Muscle injury model:

… One hour after the first immunisation, pertussis toxin (500ng in 100l saline; List Biological Laboratories, Campbell, CA, USA) was intraperitoneally injected into each animal. 

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)