Bacterial Toxin Research Citations

EAE:

… For active immunization mice were immunized subcutaneously into the flanks with 200 µg MOG35-55 peptide (Anawa) emulsified in complete Freunds adjuvant (CFA; InvivoGen) and 200 ng pertussis toxin (List Biological Laboratories Inc.) intraperitoneally on day 0 and day 2 post-immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

… Pertussis toxin (200 ng; List Biological Laboratories, Campbell, CA, USA) was intraperitoneally administrated at 0 and 48hr postimmunization. EAE scores were assessed daily …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… For adoptive transfer of effector T cells, 5×106 6 Th17 or Th1 1C6 T cells 7 were injected i.p., in cold PBS (Cellgro) into NOD.Scid recipient mice, which additionally received 200 ng of pertussis toxin (List Biological Labs) i.p. on d0 and d2 post-injection. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Cells were incubated for 30 minutes at 37°C under normal cell culturing conditions, after which 10 ng/mL of pertussis AB5 holotoxin(List Biological Laboratories Inc., 179A) was added to the cells and incubation was continued for 2 h. …

• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Primary human cell cultures:

Human skin samples were obtained as anonymised surgical waste, typically from abdominoplasty or mammoplasty. Briefly, skin biopsies were minced and trypsinized (0.05% trypsin/0.01%EDTA) at 37C for 4 hr. Cells were collected every 30 min, plated (2.5  10^4/cm²) on lethally irradiated 3T3-J2 cells (2.4 X 10⁴/cm²) and cultured in 5% CO2 and humidified atmosphere in keratinocyte growth medium (KGM): DMEM and and Hams F12 media (3:1 mixture) containing Fetal Bovine Serum (FBS) (10%), insulin (5 g/ml), adenine (0.18 mM), hydrocortisone (0.4 g/ml), cholera toxin (CT, 0.1 nM), …

STAR★Methods
Key Resources Table
Chemicals, Peptides, and Recombinant Proteins
QD Cholera Toxin List Biological Cat# 9100B

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… Macrophages were activated with Salmonella minnesota R595 LPS (100 ng/ml; List Biological Laboratories) 4 hours before nigericin treatment or 16 hours before bacterial infection, unless otherwise noted. …

• Product #304 – LPS from Salmonella minnesota R595 (Re)

EAE induction, scoring, analysis and in vitro cell cultures:

Peptide-induced EAE was induced in SJL mice and C57BL/6 mice as previously reported [11,12]. … Mice also received 200ng of Bordetella pertussis (List Biological Lab) i.p. on the day of immunization and 2days later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… CT (from V. cholerae) was purchased from List Biological Laboratories (Campbell, CA), …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Experimental Procedure:

On P10, cross-fostered ferret kits were randomized to receive an intraperitoneal 5 mg/kg dose of LPS (from Escherichia coli O111:B4, List Biological, CA, USA) or saline vehicle, before being returned to their jills for 4 h. LPS-injected animals were subsequently placed in a humidified chamber within a water bath, and underwent consecutive hypoxia, hyperoxia, and hypoxia (60 min at 9% O2, 120 min at 60% O2, and 30 min at 9% O2). …

• Product #201 – LPS from Escherichia coli O111:B4

Induced Model of Murine SLE:

This study was carried out in accordance with the recommendations of the Canadian Council on Animal Care. The protocol was approved by McGill University Animal Care Committee. C57BL/6 mice were obtained from Harlan Sprague Dawley Inc. (Indianapolis, IN, USA) and housed in a specific-pathogen-free animal facility at the animal facility of the Research Institute of the McGill University Health Center. Female (1012-weeks-old) mice were injected intravenously (i.v.) with 100 L human 2-GPI (20 g) (Crystal Chem Inc., Elk Grove Village, IL, USA), followed 24 h later by a 100 L i.v. injection of lipopolysaccharide (LPS from E.coli, serotype O111:B4; 10 g) (List Biological Laboratories, Campbell, CA, USA). 2-GPI and LPS injections were repeated every 2 weeks for a total of three rounds of immunizations, and then at 2-month intervals for the fourth and the fifth immunizations. C57BL/6 mice injected i.v. with PBS and LPS following the same schedule were used as controls. Mice were bled 1 week after the fifth immunization and serum was kept frozen at 70C until testing.

• Product #201 – LPS from Escherichia coli O111:B4