Bacterial Toxin Research Citations

… Pertussis toxin was purchased from List Biological Laboratories (Campbell, CA). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Mice:

C57BL/6J (stock #000664), MHCII^fl/fl (stock #013181) [89], and Arg1^fl/fl (stock #008817) [90] mice were purchased from the Jackson Laboratory (JAX). CCR2^-/- mice (JAX stock #004999) [34] were generously provided by Dr. E Pamer (MSKCC). Rosa26^flSTOP-tdRFP mice [36] were generously provided by Dr. J. Sun (MSKCC) [91]. The dblGATA mice [50] on a C57BL/6 background were generously provided by Dr. H. Rosenberg (NIH). Vav1-Cre mice (JAX stock #008610) [92] were generously provided by Dr. F Geissmann (MSKCC). RAG2^-/-c^-/- mice (stock #4111) [93, 94] were purchased from Taconic. CD45.1⁺ mice (stock #564) were purchased from Charles River Laboratories. The CCR2-DTR depleter mice and CCR2-GFP reporter mice were generated as previously described [5, 95]. All mouse strains were bred and housed in the Memorial Sloan Kettering Cancer Centers (MSKCC) Research Animal Resource Center under specific pathogen-free conditions. Mice on the RAG^-/-c^-/- background were maintained on amoxicillin- and Vitamin E-containing chow. To ablate monocytes, CCR2-DTR or CCR2-DTR RAG^-/-c^-/- mice and their control littermates were injected intraperitoneally with 200 ng (10 ng/g body weight) of diphtheria toxin (List Biological Laboratories) every other day for three doses, starting the day before infection (Fig 1B), unless otherwise noted. All experiments were conducted using male and female mice at age 68 weeks with sex- and age-matched mice in experimental and control groups. Experiments with CCR2-DTR, CCR2-DTR RAG^-/-c^-/-, CCR2-Cre and Vav1-Cre mice used littermate control mice that were weaned from the same litters and co-housed.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Mice:

For selective macrophage depletion, CD11b-DTR mice were injected intravenously (i.v.) with 30ng/g body weight of DT (List Biological Labs) at 36h post APAP challenge.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

EAE induction:

For EAE induction, Th/+ animals were immunized s.c. with 50g MOG₃₅₅₅ per animal emulsified in CFA substituted with 5mg/ml H37Ra (DIFCO, Detroit, MI, USA). Four hundred nanograms of pertussis toxin (PTX) per animal (List Biological Laboratories, Campbell, CA, USA) were injected i.p. twice on day 0 and day 2 relative to immunization. EAE was induced in Rag1^/ mice by the adoptive transfer of 10 Mio MOG-specific 2D2 T cell blasts i.p., and animals were immunized s.c. with 10g MOG₃₅₅₅ in CFA the following day. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Combined anterograde tracing and retrograde tracing:

Ipsilateral injections of BDA (Thermo Fisher Scientific, Waltham, MA, USA) into the LPB and CTb (List Biological Labs, Campbell, CA, USA) into the VTA (4 out of 10 rats received a successful injection), DR (4 out of 10 rats received a successful injection), PPT (4 out of 14 rats received a successful injection), LDT (4 out of 14 rats received a successful injection) or LC (4 out of 12 rats received a successful injection) were made stereotaxically by iontophoresis. In each rat, a single injection of CTb into these regions was made using a glass micropipette filled with 0.5% solution of CTb dissolved in 0.05M phosphate buffer (PB; pH 6.0). The driving current (56mA, 200ms, 2Hz) was delivered for 30-40min. After CTb injection, a single injection of BDA was made into the LPB via a glass micropipette filled with a 10% solution of BDA dissolved in 0.01M PB (pH 7.3). The driving current (56mA, 200ms, 2Hz) was delivered for 4060min.

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… Clostridium difficile toxin A was from List Biological Laboratories, Inc. (Campbell, CA). …

• Product #152C – Toxin A from Clostridium difficile

Immunization and challenge.

Mice were lightly anesthetized by the use of isoflurane for intranasal administration. For immunization with D27-pLpxL, mice were primed with intranasal inoculation of 2 × 106 CFU D27-pLpxL in 30 μl saline solution, boosted with the same dose of D27-pLpxL 30 days later, and rested for 60 days before challenge. For peptide immunizations, mice were inoculated intranasally with a 15-μl solution containing 1 μg cholera toxin (CT; List Biological Laboratory, Campbell, CA) as the adjuvant
and …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… Each mouse also received 500 ng in 200µl of pertussis toxin (PTX) (List Biological Laboratories) intraperitoneally on days 0 and 2 after immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

… Following immunization 250 ng pertussis toxin (List Biological Laboratories, California) was given intraperitonealy (IP) and again 2 days later. Seven days following immunization mice received a second MOG immunization as described above in two alternate …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… 1 µl of 0.2% Cholera toxin subunit b (CTb; List Biological Labs, Campbell, CA, USA) was pressure injected into the right VPMpc (1.9 mm anterior, 0.6 mm lateral, 4.2 mm ventral relative to bregma). …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt