Bacterial Toxin Research Citations

EAE induction:

Female mice were immunized by subcutaneous injection of 200 ml emulsion (100 mg MOG3555 peptide [MEVGWYRSPFSRVVHLYRNGK, Sigma-Aldrich] in complete Freunds adjuvant emulsion [CFA, Difco] containing 500 mg Mycobacterium tuberculosis H37Ra [Difco]). Mice were injected 100 ng pertussis toxin (List Biological Laboratories) intravenously 0 hr and 48 hr after immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

C57/BL6 mice were immunized (s.c. injection near inguinal LNs- 50ul per side) with 100ul/mouse of a 1:1 emulsion of Complete Freunds Adjuvant (CFA): Myelin oligodendrocyte glycoprotein (MOG) peptide amino acids 3535 (MEVGWYRSPFSRVVHLYRNGK), (MOG₃₅₅₅, Stanford Protein and Nucleic Acid Facility, Stanford, CA). CFA consisted of 5mg/ml mTB (Mycobacterium-tuberculosis H37 Ra, BD Difco) in IFA (Incomplete Freunds Adjuvant, Sigma). MOG₃₅₅₅ was reconstituted at 2mg/ml in PBS. Mice also received 200ng pertussis toxin (List Biological Laboratories, Inc) by i.p. injection at the time of immunization and two days later. 

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

… 200 ng of pertussis toxin (List Labs, USA) was applied intraperitoneally (ip) on day 0 and 2 after immunization. Control mice were treated without MOG. All of the mice were weighted and scored daily as follows …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Purified (low protein and nucleic acid content) and lyophilized LPS from E. coli J5 strain was purchased from both List Biological Laboratories, Inc. (Campbell, CA) …

• Product #301 – LPS from Escherichia coli J5 (Rc)

EAE:

… A total of 250 ng of pertussis toxin (List Biologicals, Campbell, CA) was administered by i.p. injection at the time of immunization and 48 hours later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Drugs:

… PTX was obtained from List Biological Laboratories (Campbell, CA). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Goat polyclonal anti-Cholera Toxin, B-Subunit List Biologicals RRID: AB_10013220; Cat#703 …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

EAE induction and Evaluation:

… Two hours following immunization, mice were given 200ng pertussis toxin (List Biologicals, Campbell, CA) via intraperitoneal injection, while 400ng was given i.p. on day 2. EAE was induced in 810-week-old female SJL/J mice similarly; however, myelin proteolipid protein peptide (PLP₁₃₉₁₅₁) was used to immunize these mice and 4mg/mL Mycobacterium tuberculosiswas added to CFA. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

… After that, the mice received an intraperitoneal injection of 300 ng pertussis toxins (List Biological Lab, USA) on days 0 and 2 post immunization. Then, EAE mice were divided …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

HEK293 cell transfection and stimulation:

… For TLR4/TLR5 co-transfections, HEK293 cells stably transfected with MD-2, CD14, and TLR4 were obtained from Invivogen (Catalog # 293-htlr4md2cd14). Cells were cultured in a 96-well flat-bottomed tissue culture plate at 50 10³ cells/well in cell growth media (DMEM, 4.5 g/l glucose, 50 U/ml penicillin, 50 g/ml streptomycin, 100 g/ml Normocin, 10 g/ml blasticidin, 50 g/ml Hygromycin B Gold, plus 10% fetal bovine serum (FBS) and 1% L-glutamine). The following day cells were transiently transfected using PolyFect transfection reagent (Qiagen, Valencia, CA) and the following DNA: NF-B-ELAM firefly luciferase 50 ng/well, HSV thymidine kinase-Renilla luciferase (pRL-TK, Promega) 5 ng/well, and huTLR5 (cloned into pEF6) and empty pEF6 vector at 110 ng/well as indicated. The transfection complexes were allowed to incubate with the cells for 24 hours. The following morning, the media in the wells was aspirated and the cells were then stimulated with ultrapure Escherichia coli O111:B4 LPS (List Biologicals, Campbell, CA) or ultrapure S. Typhimurium flagellin (Invivogen) suspended in cell growth media. After four hours, cells were lysed with passive lysis buffer and NF-B activation was determined as above.

• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4