Bacterial Toxin Research Citations

… After baseline measurements, endotoxin was intravenously infused throughout the experiment to model either moderate (10 ng/kg/min, Escherichia coli O55:B5, List Biologic Laboratories Inc, Campbell, California) or mild (2.5 ng/kg/min) endotoxemic states. …

• Product #203A – LPS from Escherichia coli O55:B5

Tetanus Neurotoxin Preparation:

… We acquired tetanus neurotoxin from clostridium tetani in solid form from multiple vendors (List Labs, …

• Product #190 – Tetanus Toxin from Clostridium tetani

Reagents – … Cholera toxin (CT) was purchased from List Biological Laboratories Inc. (Campbell, CA).

Experimental set-up: oral sensitization,immunotherapy, and challenges of mice:

The experimental design is depicted in Figure 1. After randomization and acclimatization (1 week), mice were sensitized intragastrically (i.g.) to PE (6 mg in 200 L PBS) using CT (15 µg/mouse) as an adjuvant, according to the method described by van Wijk et al (day 0, 1, 2, 7, 14, 21, and 28).18S ham-sensitized mice were treated with CT in PBS alone.  Starting on day 42, the mice were dosed i.g. with theoral immunotherapy treatment (OIT, 15 mg PE in 500 LPBS) five times/week, for 3 weeks (day 4260).  Sham-sensitized and allergen-sensitized control mice were treated i.g. with PBS alone. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Chemicals and cereals:

… Cholera toxin was obtained from List Biological Laboratories …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Chemicals and reagents:

… Cholera toxin was purchased from List Biological Laboratories, Inc. (Campbell, CA, USA). …

Results – 

Effect of CAR activation on cholera toxin (CT)-induced Cl secretion in T84 cells:

As activation of CFTR-dependent Cl secretion is involved in the pathogenesis of secretory diarrhea especially cholera [23], the therapeutic potential of CAR agonist on cholera toxin (CT)-induced Cl secretion by T84 cell monolayers was investigated using short-circuit current analysis. Snapwell inserts containing confluent T84 cells were treated with 1M CITCO for 24h followed by mounting in an Ussing chamber. Then, 1g/ml of CT was added into apical Krebs buffer to stimulate Clsecretion. As demonstrated in Fig. 8, CT-induced Cl secretion in T84 cells was significantly reduced by CITCO (78.66.0% of control).

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… Goat polyclonal anti-Cholera Toxin, B-Subunit List Biologicals RRID: AB_10013220; Cat#703 …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

… Pertussis toxin was purchased from List Biological Laboratories (Campbell, CA). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Mice:

C57BL/6J (stock #000664), MHCII^fl/fl (stock #013181) [89], and Arg1^fl/fl (stock #008817) [90] mice were purchased from the Jackson Laboratory (JAX). CCR2^-/- mice (JAX stock #004999) [34] were generously provided by Dr. E Pamer (MSKCC). Rosa26^flSTOP-tdRFP mice [36] were generously provided by Dr. J. Sun (MSKCC) [91]. The dblGATA mice [50] on a C57BL/6 background were generously provided by Dr. H. Rosenberg (NIH). Vav1-Cre mice (JAX stock #008610) [92] were generously provided by Dr. F Geissmann (MSKCC). RAG2^-/-c^-/- mice (stock #4111) [93, 94] were purchased from Taconic. CD45.1⁺ mice (stock #564) were purchased from Charles River Laboratories. The CCR2-DTR depleter mice and CCR2-GFP reporter mice were generated as previously described [5, 95]. All mouse strains were bred and housed in the Memorial Sloan Kettering Cancer Centers (MSKCC) Research Animal Resource Center under specific pathogen-free conditions. Mice on the RAG^-/-c^-/- background were maintained on amoxicillin- and Vitamin E-containing chow. To ablate monocytes, CCR2-DTR or CCR2-DTR RAG^-/-c^-/- mice and their control littermates were injected intraperitoneally with 200 ng (10 ng/g body weight) of diphtheria toxin (List Biological Laboratories) every other day for three doses, starting the day before infection (Fig 1B), unless otherwise noted. All experiments were conducted using male and female mice at age 68 weeks with sex- and age-matched mice in experimental and control groups. Experiments with CCR2-DTR, CCR2-DTR RAG^-/-c^-/-, CCR2-Cre and Vav1-Cre mice used littermate control mice that were weaned from the same litters and co-housed.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Mice:

For selective macrophage depletion, CD11b-DTR mice were injected intravenously (i.v.) with 30ng/g body weight of DT (List Biological Labs) at 36h post APAP challenge.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

EAE induction:

For EAE induction, Th/+ animals were immunized s.c. with 50g MOG₃₅₅₅ per animal emulsified in CFA substituted with 5mg/ml H37Ra (DIFCO, Detroit, MI, USA). Four hundred nanograms of pertussis toxin (PTX) per animal (List Biological Laboratories, Campbell, CA, USA) were injected i.p. twice on day 0 and day 2 relative to immunization. EAE was induced in Rag1^/ mice by the adoptive transfer of 10 Mio MOG-specific 2D2 T cell blasts i.p., and animals were immunized s.c. with 10g MOG₃₅₅₅ in CFA the following day. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)