Bacterial Toxin Research Citations

… Recombinant anthrax lethal factor was purchased from List Biological Laboratories, Inc. …

Author did not specify which Lethal Factor (LF) was utilized; List Labs provides the following LF:

• Product #172 – Anthrax Lethal Factor (LF), Recombinant from B. anthracis
• Product #169L – Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence

EAE induction

Mice were immunized subcutaneously in two sites (left and right flanks) with 150 μg of PLP 139–151 (New England Peptide, Gardner, MA) peptide that was emulsified in complete Freund’s adjuvant (CFA; Sigma-Aldrich) containing 200 μg Mycobacterium tuberculosis (Difco), followed by 200 ng pertussis toxin (PT; List Biological Laboratories) in 0.2 ml PBS by intraperitoneal (i.p.) injections at the time of immunization and 48 h later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… After 30min, mice were given 20mg BLG (Sigma-Aldrich) plus 10µg cholera toxin (List Biologicals). …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… For experiments with TLR7.1/Ccr2- DTR mice, mice were injected intraperitoneally with 10 ng DT/g of body weight (List Biological Laboratories) in phosphate-buffered saline every other day for 17 days.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Retrograde tracer injections in nucleus reuniens and the mammillary bodies:

A total of ten rats received retrograde tracer injections. In seven of these cases, two retrograde tracer injections were made, one directed at nucleus reuniens, the other at the mam-millary bodies (Table 1). In the three remaining cases only the injection directed at nucleus reuniens was successful. Different tracers were used, one in each site. The tracers used were fast blue (FB, SigmaAldrich, Gillingham, UK) and choleratoxin b (CTB, List Biological Laboratories Inc, CA; 1% solution in 0.05 M tris). Except for one CTB injection that was injected by iontophoresis, all retrograde tracers were injected mechanically at a rate of 20 nl/min via a 0.5 µl or 1.0 µl Hamilton pipette (Hamilton, Bonaduz, Switzerland). Individual injection volumes for mechanical injections were 50-60 nl. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Test approach:

No standardized approaches are available to investigate anthrax seroprevalence in wildlife. Thus, samples were tested for antibodies against the anthrax protective antigen (PA, List Biological Laboratories Inc) using an in-house ELISA and an in-house Western Blot and for antibodies against anthrax lethal factor (LF, List Biological Laboratories Inc) using an in-house Western Blot. …

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169L (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

… Escherichia coli lipopolysaccharide (LPS, 0111: B4, List Biological Laboratories, Campbell, CA; …

• Product #201 – LPS from Escherichia coli O111:B4

Induction and treatment of EAE:

EAE was induced in age-matched female mice with 300L of 300µg MOG emulsified in CFA containing 8mg/mL killed Mycobacterium tuberculosis via subcutaneous injection on day 0 and day 7, followed by the i.p. injection of 250ng pertussis toxin (list biological laboratories) on day 0, day 2, day 7, day 9¹³. Clinical scores (0, no symptoms; 1, limp tail; 2, partial paralysis of hind limbs; 3, complete paralysis of hind limbs or partial hind and front limb paralysis; 4, tetraparalysis; 5, moribund) were recorded daily. The mean score was noted for each mouse every day. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… QD grade anthrax lethal factor (LF-A 0.1 mg, product No. 169 A, B), (List Biological Laboratories. Inc.).

List Labs Product #169A/B (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence; Lyophilized) has been discontinued; however, Product #169L (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence; Liquid) is available for purchase.

• Product #169L – Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence

Immunohistochemistry:

… goat anti-CTB (Cat# 703, List Biological Lab, 1:4000) …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)