Bacterial Toxin Research Citations

EAE:

… Mice then received 200 ng of pertussis toxin (List Biological Laboratories) intraperitoneally (i.p.) at 4 hours and 24 hours after immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Non-immunized control mice received saline. Mice also received 10µg/kg pertussis toxin (List Biological Laboratories) intraperitoneally on the day of immunization and again 48 h later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction, Treatment, and Evaluation of Active EAE:

Female SJL/J mice were immunized subcutaneously (s.c.) in depots distributed over 4 spots across the flanks … containing 4 mg/ml of Mycobacterium tuberculosis H37Ra (Difco, Detroit, MI). 200 ng pertussis toxin (PTX; List Biological Laboratories, Campbell, CA) was administered intraperitoneally (i.p.) at days 0 and 2 [18]. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… overlaying the SC, or the lateral posterior thalamus. A glass pipette … containing either CTB (List Biological Laboratories, Campbell, CA; 1% in phosphate buffer 0.1 M) or PHA-L (2.5% PHA-L in 10 mM phosphate buffer, pH 8; …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… anti-CTB at 1:10,000 (List Biological Laboratories, Campbell, CA). …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

EAE:

EAE was induced in 7- to 13-wk-old female mice (Kihara et al., 2009). Briefly, mice were immunized subcutaneously with 150 µg myelin oligodendrocyte glycoprotein … in PBS and complete Freunds adjuvant containing 4 mg/mL M. Tuberculosis H37Ra (Difco) with or without intraperitoneal injection of 250 ng pertussis toxin (List Biological Laboratories) on days 0 and 2.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Pertussis toxin was from List Biological Laboratories (Campbell, CA). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Indirect immunofluorescence assays:

HFF cells seeded on glass cover slips were infected with freshly isolated tachyzoites for 24 h, fixed with 4 % paraformaldehyde for 10 min, and then neutralized with 0.1 M glycine in PBS for 5 min. Samples were permeabilized by 0.1 % triton X -100 in PBS for 20 min, blocked with 2 % bovine serum albumin in PBS/0.1 % triton X -100 for 20 min. Subsequently, they were stained with primary antibodies (mouse anti -HA, 1:10.000; rabbit anti -TgGap45, 1:10.000) for 1 h and then with respective secondary antibodies (Alexa488 and Alexa594, 1:3.000) for 45 min in the dark. Samples were mounted into a mix of fluoromount G and DAPI, dried at 4 C overnight and examined by fluorescence microscopy (ApoTome, Zeiss). To resolve the C -terminal topology of TgFNT1 – 3, extracellular parasites were stained with a mouse -HA antibody (1:3000), suspended either in 3% BSA/PBS solution for pre -permeabilization staining, or in 2% BSA/0.2% triton X -100/PBS for post -permeabilization, as shown in Fig. S1. The inner membrane complex was separated from the plasma membrane by treating extracellular parasites with -toxin from Clostridium septicum (20 nM, 2 h) (List Biological Laboratories) followed by fixation on BSA -coated (0.01%) coverslips. In both cases, the standard secondary antibody staining procedure was performed afterwards, as described above.

… CTb Goat 1:200,000 List Biological 703 …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

… Pertussis toxin (200 ng, List Biological Laboratories) in PBS was administered i.v. on days 0 and 2. Mice were examined daily and scored for disease severity using the standard scale: 0, no clinical signs; 1, limp tail; 2, paraparesis (weakness, incomplete paralysis of one or two hind limbs); …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)