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Anthrax Lethal Factor (LF), Recombinant from B. anthracis

Anthrax Lethal Factor (LF), Recombinant from B. anthracis

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Products are for research purposes only and are
not for use in humans or as diagnostic agents.

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Price range: $780.00 through $1,080.00

#172B (1 mg), #172L (1 mg),

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Technical Info

Certificate Of Analysis

N-HAZ

Poster

Purity

refer to the CoA

Form

refer to the CoA

Storage temp

refer to the CoA

Additional information

Product # And Size

172B (1 mg), 172L (1 mg)
Destination

,

We have 70 citations
Single-molecule sensing with an anthrax nanopore enabled by pH-asymmetric ionic liquids
Zhang, Wang, Zhou et al
J Nanobiotechnology (2025) 23 (1), 747 DOI: 10.1186/s12951-025-03817-w
Cited by 7
FigureFigureFigure
A Lyophilizable Nanoparticle Vaccine Specific for a Novel Linear Neutralizing Epitope in the α2-α3 Helices of Domain 3 of Lethal Factor from Bacillus anthracis
Oscherwitz, Cease, Milich et al
Toxins (Basel) (2025) 17 (8) DOI: 10.3390/toxins17080422
Cited by 1
Snippet: ors can be pre-incubated with LeTx to prevent the cytotoxicity of the LeTx on the susceptible RAW264.7 macrophage target cell line. For neutralization studies, approximately 120 ng/mL of PA (PA 171E, List Labs, Campbell, CA, USA) and 40 ng of LF (LF169L, List Labs, Campbell, CA, USA) were used in the TNA. Contemporaneous toxin dose 50% (TD50) were separately determined for PA and LF with each TNA
FigureFigureFigure
A Lyophilizable Nanoparticle Anthrax Vaccine Targeting the Loop-Neutralizing Determinant in Protective Antigen from Bacillus anthracis
Oscherwitz, Cease, Milich et al
Microorganisms (2025) 13 (8) DOI: 10.3390/microorganisms13081878
Cited by 1
Snippet: ... For neutralization studies, approximately 120 ng/mL of PA (PA 171E, List Labs, Campbell, CA, USA) and 25 ng LF (LF169L, List Labs, Campbell, CA, USA) were used in the TNA. Contemporaneous toxin dose 50% (TD50) was determined for PA and LF with each assay to validate ...
FigureFigureFigure
Serological correlates of protection and long-term efficacy of a novel recombinant protective antigen-based anthrax vaccine in a rabbit model
Kim, Pyo, Kim et al
Vaccine (2025) 57, 127212 DOI: 10.1016/j.vaccine.2025.127212
Snippet: … Toxin neutralization activity assay ?These were mixed with anthrax lethal toxin (500 ng/mL PA and 100 ng/mL lethal factor [LF]; List Biologicals, USA) in a separate 96-well microtiter plate at equal volumes and incubated for 30 min. For long-term immunogenicity evaluation, TNA was performed using anthrax lethal toxin at concentrations of 100 ng/mL PA and 80 ng/mL LF. …
The Anthrax Toxin Lethal Factor in Solution Does Not Have the Protein’s Crystallized Structure
Rubinson, Kasianowicz
Toxins (2025) 17 (4), 157 DOI: 10.3390/toxins17040157
Snippet: - … The List Biological Laboratories' recombinant LF used here has two additional residues (histidine and methionine) on the N-terminal, giving it a molecular mass of 90.49 …
DAG-MAG-ΒHB: A Novel Ketone Diester Modulates NLRP3 Inflammasome Activation in Microglial Cells in Response to Beta-Amyloid and Low Glucose AD-like Conditions
Gentili, Schiuma, Dilliraj et al
Nutrients (2024) 17 (1), 149-149 DOI: 10.3390/nu17010149
Cited by 7
Snippet:

2.13. Priming of Inflammasomes

Inflammasome activation was measured according to Primiano et al.'s description [28]. HCM3 cells were plated in EMEM without supplements at 1 × 105 cells/well. Cells were stimulated for 1 h with 10 g/mL (13.4 mM) of nigericin (tlrl-nig, InvivoGen, San Diego, CA, USA) for 2 h with 2 mM ATP (tlrl-atpl, InvivoGen), or overnight with 200 g/mL of monosodium urate crystals (MSU; tlrl-msu, InvivoGen) for NLRP3 induction; flagellin/DOTAP complex, obtained with 25 μL HBS buffer with 500 ng flagellin (final concentration at 1 μg/mL; tlrl-stfla, InvivoGen) and 3 μL DOTAP (11202375001, Sigma-Aldrich) for each well for NLRC4 induction; with 0.5 mL Opti-MEM supplemented with GlutaMax (Opti-MEMâ„¢ Reduced Serum Medium containing GlutaMAXâ„¢ Supplement, 51985034, Gibco, Grand Island, NY, USA) containing 1 μg/mL Bacillus anthracis lethal factor (172B, List Biological Laboratories, Campbell, CA, USA) for 3-6 h for NLRP1 induction; and with a poly(dA:dT)/lipofectamine complex, obtained by mixing 50 μL Opti-MEM containing 1 μg poly(dA:dT) (final concentration at 2 μg/mL) with 50 μL Opti-MEM containing 2.5 μL lipofectamine 2000 (11668027, Thermo Fisher's Scientific, Waltham, MA, USA) for each well for AIM2 induction. ...

NLRP1B allele 2 does not respond to Val-boro-Pro (VbP) in the intestinal epithelium
Mazzone, Winsor, Li et al
bioRxiv (2024)
Snippet:

Chemicals and reagents

... LF (List Labs), ...

Author did not specify which Lethal Factor (LF) was utilized; List Labs provides the following LF:

NLRP1B expressed in intestinal epithelial cells is refractory to activation with Val-boro-Pro
Mazzone, Winsor, Li et al
Microbes Infect (2024)
Cited by 3
Snippet:

... LF (List Labs), ...

Author did not specify which Lethal Factor (LF) was utilized; List Labs provides the following LF:

Species-specific NLRP3 regulation and its role in CNS autoinflammatory diseases
Koller, Nguyen, Snouwaert et al
Cell Reports (2024) 43 (3), 113852 DOI: 10.1016/j.celrep.2024.113852
Cited by 27
Snippet:

... Anthrax lethal toxin List Biological Laboratories Inc. #171B/#172B ...

Treatments

See Table S1 for list of Figures/panels in each figure and corresponding dose and exposure time. General information for the methods is provided here. To induce cytokine release from macrophages, microglia and BMDM cells were primed for 4-5 h (or time indicated in Table S1)with 1 μg/mL of LPS in culture medium, and some cells were pre-treated with 1 μM of CP456773/MCC950 for 30 min before ATP stimulation. Cells were then pulsed with 5 mM ATP for 30 min. For cytokine induction by anthrax lethal toxin (LeTx), LPS-primed cells were treated with LeTx at a final concentration of 250 ng/mL (LF and PA) for 3 h. Cell supernatants were collected and frozen at −80°C until assayed.

Showing 1-10 of 70 papers.
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We have 6 citations
NLRP1B is an integrated decoy that subverts Shigella flexneri E3 ligase activity to promote effector-triggered immunity
Chung, Hudson, Stromberg et al
Proc Natl Acad Sci U S A (2026) 123 (13), e2514645123 DOI: 10.1073/pnas.2514645123
Snippet: 4 μg/mL LeTx (4 μg/mL Lethal factor protease + 4 μg/mL Protective Antigen) (LIST Labs), or 1% triton 100. At harvest, 35 μL of supernatant was collected for LDH activity assay. …
Single-molecule sensing with an anthrax nanopore enabled by pH-asymmetric ionic liquids
Zhang, Wang, Zhou et al
J Nanobiotechnology (2025) 23 (1), 747 DOI: 10.1186/s12951-025-03817-w
Cited by 7
FigureFigureFigure
A Lyophilizable Nanoparticle Vaccine Specific for a Novel Linear Neutralizing Epitope in the α2-α3 Helices of Domain 3 of Lethal Factor from Bacillus anthracis
Oscherwitz, Cease, Milich et al
Toxins (Basel) (2025) 17 (8) DOI: 10.3390/toxins17080422
Cited by 1
Snippet: ors can be pre-incubated with LeTx to prevent the cytotoxicity of the LeTx on the susceptible RAW264.7 macrophage target cell line. For neutralization studies, approximately 120 ng/mL of PA (PA 171E, List Labs, Campbell, CA, USA) and 40 ng of LF (LF169L, List Labs, Campbell, CA, USA) were used in the TNA. Contemporaneous toxin dose 50% (TD50) were separately determined for PA and LF with each TNA
FigureFigureFigure
A Lyophilizable Nanoparticle Anthrax Vaccine Targeting the Loop-Neutralizing Determinant in Protective Antigen from Bacillus anthracis
Oscherwitz, Cease, Milich et al
Microorganisms (2025) 13 (8) DOI: 10.3390/microorganisms13081878
Cited by 1
Snippet: ... For neutralization studies, approximately 120 ng/mL of PA (PA 171E, List Labs, Campbell, CA, USA) and 25 ng LF (LF169L, List Labs, Campbell, CA, USA) were used in the TNA. Contemporaneous toxin dose 50% (TD50) was determined for PA and LF with each assay to validate ...
FigureFigureFigure
Serological correlates of protection and long-term efficacy of a novel recombinant protective antigen-based anthrax vaccine in a rabbit model
Kim, Pyo, Kim et al
Vaccine (2025) 57, 127212 DOI: 10.1016/j.vaccine.2025.127212
Snippet: … Toxin neutralization activity assay ?These were mixed with anthrax lethal toxin (500 ng/mL PA and 100 ng/mL lethal factor [LF]; List Biologicals, USA) in a separate 96-well microtiter plate at equal volumes and incubated for 30 min. For long-term immunogenicity evaluation, TNA was performed using anthrax lethal toxin at concentrations of 100 ng/mL PA and 80 ng/mL LF. …
The Anthrax Toxin Lethal Factor in Solution Does Not Have the Protein’s Crystallized Structure
Rubinson, Kasianowicz
Toxins (2025) 17 (4), 157 DOI: 10.3390/toxins17040157
Snippet: - … The List Biological Laboratories' recombinant LF used here has two additional residues (histidine and methionine) on the N-terminal, giving it a molecular mass of 90.49 …
Showing 1-6 of 6 papers.
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