MAPKKide® Peptide Substrate (o-Abz/Dnp) for Anthrax Lethal Factor (MAPKKide® 530) is a quenched fluorescent substrate peptide based on fluorescence resonance energy transfer (FRET) and is derived from the MAPKK-2 substrate for LF. The N-terminally-linked fluorophore is o-aminobenzoic acid (o-Abz), and the acceptor chromophore is 2,4-dinitrophenol (Dnp). This substrate can be used for development of highly sensitive and rapid in vitro methods for screening and characterizing of LF inhibitors. [more…]
MAPKKide®, Product #530, is provided as a lyophilized powder. MAPKKide® preparations are highly purified, and each shipment is provided with recommended assay conditions on the C of A. An unquenched calibration peptide for MAPKKide® is also available as Product #539.