Bacterial Toxin Research Citations

Mice and EAE:

Hdac11 constitutive KO mice were generated at the Institut Clinique de la Souris (ICS) in collaboration with Merck & Co. and obtained via Taconic. These mice were backcrossed with the C57BL/6 background for successive generations and then bred to homozygosity. WT C57BL/6 mice were used as controls for these experiments. To induce EAE, WT and HDAC11 KO female mice between 8 and 12 wk of age were immunized with MOG₃₅₅₅ peptide emulsified with CFA (Sigma-Aldrich)-containing Mycobacterium tuberculosis (200 ng/ml; Difco). For each animal, 100 µl of emulsion per site was deposited subcutaneously in the upper and lower backs. In addition, each animal was administered pertussis toxin (islet-activating protein, 500 ng, i.p.; List Biological Laboratories) at the time of immunization and again 1 d later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Pertussis toxin (200 ng, List Biologicals) was injected i.p. on day 0 and day 1 after MOG immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Diphtheria toxin (DT; 50 g/kg body wt; List Laboratories, Campbell, CA) was administered once (in the absence of UUO) or at stated times after UUO via ip doses …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… 0.2 µl of CTb (10 µg/ml, List Biological Laboratories, CA, USA) was injected into the vHPC, …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… Cholera Toxin from Vibrio cholerae, List Biological Labs, Cat# 100B. Binary Toxin from Clostridium difficile, Subunit A, List Biological Labs, Cat# 157A. Binary Toxin from Clostridium difficile, Subunit B, List Biological …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Induction of Experimental Autoimmune Encephalomyelitis (EAE) in female C57BL/6 mice:

Experimental autoimmune encephalomyelitis (EAE), a frequently used mouse model of multiple sclerosis (Robinson et al, 2014), was induced in female C57BL/6 mice largely as previously described (Williams et al, 2011). For EAE induction, female C57BL/6 mice older than 10 weeks (body weight between 20 and 25 g) were selected. Mice were injected subcutaneously into the axilla and groin with a total of 200 g of the encephalitogenic MOG₃₅₅₅ peptide of mouse myelin oligodendrocyte glycoprotein (MEVGWYRSPFSRVVHLYRNGK; > 90% purity, synthesized by Dr. Martin Jung, Institute of Medical Biochemistry and Molecular Biology, Medical School Homburg, Saarland University) in sterile water (2 mg/ml) emulsified with an equal volume of complete Freund adjuvant (CFA), consisting of incomplete Freund adjuvant (iCFA, Sigma; #F5506) to which 10 mg/ml inactivated Mycobacterium tuberculosis were added (Fisher Scientific #10218823), as previously described (Williams et al, 2011). 200 ng of pertussis toxin (PTX) from Bordetella pertussis (List Biological Laboratories Inc. #181, via Biotrend, Cologne, Germany) in a volume of 100 l sterile H₂O was injected intraperitoneally on the same day (day 0, 12 h after MOG₃₅₅₅ peptide injection) and also on the subsequent day (day 1, 1620 h after first PTX injection). …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

EAE:

… Mice then received 200 ng of pertussis toxin (List Biological Laboratories) intraperitoneally (i.p.) at 4 hours and 24 hours after immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Non-immunized control mice received saline. Mice also received 10µg/kg pertussis toxin (List Biological Laboratories) intraperitoneally on the day of immunization and again 48 h later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction, Treatment, and Evaluation of Active EAE:

Female SJL/J mice were immunized subcutaneously (s.c.) in depots distributed over 4 spots across the flanks … containing 4 mg/ml of Mycobacterium tuberculosis H37Ra (Difco, Detroit, MI). 200 ng pertussis toxin (PTX; List Biological Laboratories, Campbell, CA) was administered intraperitoneally (i.p.) at days 0 and 2 [18]. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… overlaying the SC, or the lateral posterior thalamus. A glass pipette … containing either CTB (List Biological Laboratories, Campbell, CA; 1% in phosphate buffer 0.1 M) or PHA-L (2.5% PHA-L in 10 mM phosphate buffer, pH 8; …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt