Bacterial Toxin Research Citations

LPS was purchased from List Biological Laboratories, …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

EAE:

… Mice also received 300 ng of Pertussis toxin (List Biological Laboratories) iv on the day of immunization and 2 days later. EAE score was …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Pertussis toxin (List Biological Labs) in 100 ul saline was injected. intravenously twice, on days 0 and 2, and disease severity was assigned scores. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

EAE was induced in 8-week-old mice by subcutaneous immunization with 150g µ myelin oligodendrocyte glycoprotein (MOG3555) peptide (Genemed Synthesis Inc.) emulsified in complete Freunds adjuvant (CFA, Difco Laboratories) per mouse, followed by administration of 200ng pertussis toxin (PTX, List Biological Laboratories, Inc.) on days 0 and 2 as described …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Reagents:

… The CTb was from List Biological Laboratories (Campbell, CA, USA). …

Intraocular injections:

Syrian golden hamsters (Charles River) were used for investigating the in vivo implication of succinate/GPR91 and -KG/GPR99 in RGC projection growth during postnatal development. At P1, 24 h after birth, anesthetized hamsters received a unilateral injection of 2 µl solution of CTb with either 0.9% saline solution, succinate (100 mM), or -KG (200 mM). …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Antigen recall assay:

… Antibodies were prepared at 4 concentrations and added to the cells at 50 l per well. After 1-h incubation, Tetanus Toxoid (50uL; 0.8g/ml) (TT; #191A LIST Biological Laboratories Inc.) was added to wells with LY3300054 or medium control. …

• Product #191A – Tetanus Toxoid from Clostridium tetani

… For induction of EAE, experimental mice (aged 68 weeks old) were injected subcutaneously in the flanks with 200µL of 1mM MOG 3555 peptide (DNA/Peptide Synthesis Core Facility, University of Utah) emulsified with reconstituted complete Freunds adjuvant (Pierce Biotechnology, Waltham, MA, USA) containing Mycobacterium tuberculosis H37 Ra (2 mg/mL) (Difco Laborato-ries, Franklin Lakes, NJ, USA). Mice were injected intravenously with 100µL Bordetella pertussis toxin (Ptx) (List Biological Laboratories Inc., Campbell, CA, USA) …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Anthrax lethal factor and edema factor were from List Biological Laboratories (Campbell, CA). …

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence)

Author did not specify which List Labs Anthrax Edema Factor was utilized.  List Labs provides Product #178A(Anthrax Edema Factor (EF), Recombinant from B. anthracis) and Product #173B (Anthrax Edema Factor (EF), Recombinant, Mutant S447N from B. anthracis).

Induction of EAE:

EAE was induced as previously described [12]. All procedures were approved by Uniformed Services University Institutional Animal Care and Use Committee. Briefly, each male C57BL/6J (The Jackson Laboratory, 9 to 16 weeks old) was injected subcutaneously with PBS (Control) or 100 µg MOG_35-55 (New England Peptide) emulsified in 100 µl complete Freunds adjuvant containing 300 g Mycobacterium tuberculosis (Fisher Scientific) and 100 ng pertussis toxin (List Biological Laboratories) intraperitoneally. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction and Assessment of EAE:

C57BL6, IL-10^/, and 2D2 mice of 812 weeks of age were immunized subcutaneously with 200 g MOG 35-55 emulsified in PBS:CFA (1:1) in a total volume of 100 µL. … Mice also received 150 ng of pertussis toxin (List Biological, Campbell, CA, USA) in 200 L PBS via the tail vein immediately after the immunization and again 48 h later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)