Bacterial Toxin Research Citations

– Dermatophagoides pteronyssinus (Der p1; Greer Laboratories, Lenoir, NC, USA) and SEB from Staphylococcus aureus (List Biological Laboratories, Inc., Campbell, CA, USA) was adapted from Khalmuratova et al. (20). Statistical analyses were performed by a statistician …

… Pertussis toxin (500 ng, List Biological Laboratories, Campbell, CA, USA) was injected i.v. on the day of the immunization and again 2 days later …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Mice were orally sensitized with 20 mg of homogenized whey (WPC60, Milei, Friesland Campina, Amersfoort, The Netherlands) mixed with 10 µg of the adjuvant cholera toxin (List Biological Laboratories, Campbell, USA) in 0.5 ml PBS, once-weekly, …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

EAE:

… Pertussis toxin, 200 ng, (List Biological Laboratories, Inc., 154 Campbell, CA) was injected ip on the day of EAE induction and again two days …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Quantitation of Axons, CSPG levels, and Retinal Thickness:

Axons were visualized at 2 weeks after optic nerve injury by immunostaining with the anti-CTB antibody (1:500, rabbit polyclonal; GenWay, #GWB-7B96E4) for the cholera toxin B (CTB, 1% in 3 µl PBS; List Biological, #103B) fragment, which was injected intravitreally 1 day prior to sacrifice. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… On day 0, mice also received 500 ng pertussis toxins intraperitoneally (List Biologicals) [75]. Mouse hearts were harvested on days 0, 14 or 21 for flow cytometry analysis. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Diphtheria toxin List Biological Labs Part# 150

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Quantification of R-CTB and R-CTB by densitometry:

Known concentrations of purified CTB (List Laboratories) were resolved by SDS-PAGE alongside R-CTB or R-CTB samples (plasmid and chromosomal) and used as standards for quantification. ImageJ was used to generate a standard curve fitted to the intensities of bands corresponding to the CTB standards. The concentrations of R-CTB and R-CTB were calculated using the linear portion of the standard curve.

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Materials:

… List Labs , #703; …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Breakdown of BBB and Passive Transfer of Human IgG to Mice:

Female wild-type C57BL/6 mice of age 68 weeks were used. …

Procedures of the animal experiments were summarized in Figure 1A. … In addition, mice received two intraperitoneal (i.p.) injections of pertussis toxin (PTx, List Biological Laboratories, USA), 200 ng in 0.2 ml PBS each, 7 and 3 days before IgG transfer. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)