Bacterial Toxin Research Citations

… Injection of retrograde tracer into preBotzinger complex In three animals, preBotC injections of the retrograde tracer, Cholera Toxin b (CTb, 1% in deionized water; List Biological, Campbell, CA) were unilaterally performed. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Peptide:

Tetanus Toxoid (Lot #19119A1) List Biological Laboratories, Campell, USA

ELISpot Assay:

… As negative controls served four cell suspension containing
Holes without further additions and two holes each, in which DMSO in the same
Dilution as the dissolved in DMSO peptides were added. As positive
Control antigens were tetanus toxoid at a concentration of 0.3 g / ml and
Phytohemagglutinin (PHA) at the concentration of 3 g / ml. …

• Product #191 – Tetanus Toxoid from Clostridium tetani

For retrograde tracing, 1 µl of cholera toxin B subunit (CTB) solution (10% dilution with ddH2O; List Biological Laboratories), …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

INDUCTION OF EPILEPSY: TETANUS NEUROTOXIN INJECTION:

… Each animal was anaesthetised and placed into a stereotaxic frame. 2.5 ng TeNT (List Biological Laboratories Inc., United States) in 1 L of 2% bovine serum albumin in phosphate buffered saline was injected through the guide cannula, at 200 nL min-1 using a Hamilton Syringe (25G, 7001 KH, Hamilton Company, United States) and a microinjection pump (WZ-74905-02, Cole-Parmer, United Kingdom).

• Product #190 – Tetanus Toxin from Clostridium tetani

… Goat anti-CTb, List Biological Labs, Cat#703. …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Goat anti-CTB List Biological Laboratories Cat# 703; …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

… For FAP+ cell deletion, Diphtheria Toxin (DTx) (List Biological Laboratories) was administered by 390 i.p. injection to 8 week old FAP-DTR mice at a dose of 25ng/g, twice a day, in a prophylactic regime (at days -7 and -5 post STIA) and in a therapeutic regime (at days 3 and 5 or at days 5 and 7 post STIA or at days 10 and 12 393 post STIA). …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

REAGENTS AND SOLUTIONS:

Anthrax protective antigen and anthrax lethal factor, 1 mg/ml

Dissolve anthrax lethal factor and anthrax protective antigen (List Biological Labo-

ratories, cat. no. 172B and 171E) each to a nal concentration of 1 mg/ml in sterile

dH2O. Divide into 10-l aliquots or an appropriate volume for the users experiments

and store up to 2 years at 80C. Avoid freeze-thaw cycles. Remove one aliquot of

each from the freezer 30 min prior to starting the experiment. Allow them to come to

room temperature. Enzymatically inactive anthrax lethal factor (List Biological Lab-

oratories, cat. no. 176) can be used as a negative control, if desired. Good biosafety

level 2 laboratory practices should be employed in the safe handling of this reagent.

Protective antigen and lethal factor should be stored separately.

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis
• Product #176 – Anthrax Lethal Factor (LF), Recombinant, Mutant E687C from B. anthracis

LPS, 1 mg/ml

Prepare a 1 mg/ml stock of Salmonella Minnesota R595 (Re) LPS (e.g., List Bi-

ologicals, cat. no. 434) in H2O containing 0.5% triethylamine. Divide into 100-l

aliquots and store up to 1 year at 4C.

• Product #434 – ULTRA PURE LPS from Salmonella minnesota R595 (Re)

… Control mice received only CFA. On day 0, experimental and control mice were also immunized with 50 ng of pertussis toxin (List Biologicals, Campbell, CA). The mice were killed at 1, 2, or 3 weeks after …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Pertussis toxin was purchased from List Biological Lab (Epsom, England). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)