Bacterial Toxin Research Citations

EAE induction and Treatment:

Female C57BL/6 mice of 812 weeks old were immunized subcutaneously with synthetic peptide derived from myelin oligodendrocyte glycoprotein (MOG₃₅₅₅) peptide (MEVGWYRSPFSRVVHLYRNGK, AnaSpec). Immunization was performed by mixing MOG₃₅₅₅ peptide in CFA containing 10mg/ml heat-killed H37Ra, strain of Mycobacterium tuberculosis (Difco Laboratories). Mice were immunized intraperitoneally with 250ng pertussis toxin (List Biological Laboratories) on the day of MOG injection and then again 48hours later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Mice were anesthetized by isoflurane and dorsal fur was removed with a clipper. A sub-cutaneous injection of 106 PDA cells was performed. Tumors were grown for 2 weeks. In order to eliminate the tumors, diphtheria toxin (DTx, List Biologicals, #150) was injected intraperitoneally on two consecutive days at a dose of 25ng/g. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… allocated into three groups: Control group without scar tissue removal (n = 6); Only scar tissue removal group (n = 6) and Scar tissue removal along with growth factor … Cholera toxin B (CTB) (List Biological Laboratory …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Intraocular Tracer Injection:

Bats were anesthetized with a pharmacological mixture of tramadol hydrochloride (5 mg/kg i.m.), ketamine (5 mg/kg i.m.), diazepam (0.5 mg/kg i.m.) and xylazine (0.5 mg/kg i.m). While maintained at the appropriate level of anesthesia, the animals received, via needle attached to a micropump, 15 µl injections of unconjugated CTb (List Biological Laboratories, Campbell, CA, United States) in one eye.

The monocular injections were administered into the vitreous humor of the left eye and contained 1% CTb mixed with 5% dimethyl sulfoxide. Five days post-injection, the bats were anesthetized with the same anesthetic protocol as the surgical procedure and subjected to the following procedures.

Perfusions and Immunohistochemistry:

… Frozen brains were cut into 30-m coronal sections. Slices with SCN were collected from 0.45 mm to 1.08 mm post-bregma, approximately at the same level of the anterior commissure in the rostral section. On the other hand, slices containing IGL were collected from 3.84 mm to 5.04 mm post-bregma, approximately at same level of the external medullary lamina in the rostral level. All sections were collected in PB, and a one-in-six series was processed for CTb. Floating sections were incubated with goat anti-CTb antiserum (List Biological Laboratories, Campbell, CA, United States) in PB containing 0.4% Triton X-100 and 5% normal donkey serum for 1824 h. The sections were incubated with a secondary antiserum (biotinylated donkey anti-goat, Jackson Immunoresearch Laboratories, West Grove, PA, United States) in the same medium as above for 90 min.

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt
• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

In vivo studies to determine PA removal from rat blood:

… PA (List Biological Laboratories, US) was administered by intraperitoneal (IP) injection (100g/kg) or infused via the venous catheter for 1hour at 100g/kg. In the filter studies, PA was infused as before for 1hour prior to connection of the extracorporeal circuit and continued at 40g/kg for the remaining 2hours of the study. After the initial 1-hour infusion period, the arterial catheter was connected to either a Valortim antibody bound or blank control cryogel columns (5cm by 1cm (length x diameter)) via a peristaltic pump. …

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Oral protocol with adjuvant:

Mice were orally exposed to 5 mg of EW (2 EW groups), 2.5 mg of EY (EY group) or 5 mg of EW plus 2.5 mg of EY (1:0.5 w:w, EW:EY group) and 10 µg of cholera toxin (CT, List Biologicals, Campbell, USA) in PBS [12]. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Assessment of Glutamate Release from Rat Cortical Neurons Treated with Botulinum Neurotoxin:

Glutamate release was assessed in cortical neurons at DIV 1921. Cortical neurons were treated with a concentration-range of nBoNT/A (LIST Laboratories, Campbell, CA, USA), …

Measurement of memory B cell responses:

… On the day prior to testing for MBC, 96-well ELISPOT plates (Millipore MIPS4150) were coated overnight at 4 °C with antigen or capture antibody. Coating antigen consisted of LPS from V. cholera, Inaba 569B serotype (University of Maryland Center for Vaccine Development; 50 µg/mL) or cholera toxin B (CtxB) (List Biological Laboratories; 15 µg/mL). Assay wells receiving CtxB were first pre-coated with GM1 ganglioside (Enzo Life Sciences; 3 nM) for 3 h at room temperature. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… Escherichia coli lipopolysaccharide (O55:B5) was purchased from List Biologicals (Campbell, CA, USA), and prepared in phosphate-buffered saline. …

NOTE:  At the time this paper was written, Product #203 (5mg – LPS from Escherichia coli O55:B5) was utilized.

Product #203 (5 mg – LPS from Escherichia coli O55:B5) is no longer sold.

Product #203A (2.5 mg – LPS from Escherichia coli O55:B5) is available for purchase.

• Product #203A – LPS from Escherichia coli O55:B5

EAE induction and treatment protocol:

Chronic EAE was induced in 68 weeks old C57Bl/6 mice by subcutaneous immunization with 300µg of MOG3335 peptide in complete Freuds adjuvant (Becton Dickinson) containing 0.8mg/ml Mycobacterium Tuberculosis (Becton Dickinson), as previously described42,43. Mice were also injected intravenously (i.v.) with pertussis toxin (40ng; List Biological Laboratories) at the day of immunization and after 48hours. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)