Bacterial Toxin Research Citations

Reagents and antibodies:

Cholera toxin (100) and insulin (16634) were from List Biological (Campbell, CA, USA) and Invitrogen (Carlsbad, CA, USA), respectively. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… In addition, the mice received 100 ng of pertussis toxin (List Biological Laboratories) iv on days 0 and 2 and were treated ip with 100 mg of anti-TIGIT (clone 4D4, 1G9, or 1B4) or isotype control (Arme- nian hamster IgG or mouse IgG1) on days 0, 2, 4, 10, and 17 …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Cell Stimulation and ERK Phosphorylation

Jurkat cells stably expressing wild-type CCR6 (WT-CCR6) or mutant CCR6 were fasted at a density of 2 × 106 cells/ml for 4–6 h in fasting medium (RPMI medium containing 0.5% BSA, 2 mM l-glutamine, 0.1 mM non-essential amino acids, 1 mM sodium pyruvate, 5.5 µM β-mercaptoethanol, and 10 mM HEPES) with or without 0.1 µg/ml pertussis toxin (PTx) (List Biological Laboratories, Campbell, CA, USA) in a 37°C incubator. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… For retrograde tracing, Fluorogold (FG; 1% 2-hydroxy-4.4-diamino-stilbene in 0.1 M cacodylate buffer, pH 7.4; Fluorochrome, Denver, CO), subunit B of cholera toxoid (CtB; 1% in distilled water; List Biological, Campbell, CA) …

List Labs does not supply CTB as a toxoid; however, the B-subunit of Cholera Toxin is available for purchase:

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… Sections were then incubated in 1:4000 dilution of CTB primary antibody (Product #703, List Biological Laboratories Inc., Campbell, CA) in 2.5% normal rabbit serum, 2% TBS+ for four days at 4 C. Following primary incubation, sections were …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Table 1 Antibodies used in this study:

… CTb Goat 703 1:5000 1:200,000 List biological …

Neurochemical characterisation of neurons in L5 that were retrogradely labelled from T13/L1 segments:

… For the analysis of eGFP expression among retrogradely labelled neurons, we used sections that had been reacted with NeuN and CTb antibodies, and counterstained with DAPI. …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Induction of LPS E. coli:

Induction of E. coli LPS (List Biological, catalog number 201) performed orally. LPS is diluted with 0.9 NaCl solution …

• Product #201 – LPS from Escherichia coli O111:B4

EAE Induction:

… Pertussis Toxin from Bordetella pertussis List Biological Laboratories 181 …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

EAE:

… On days 0 and 1 post-challenge, each mouse received 400 ng of Bordetella pertussis toxin intraperitoneally (List Biological Laboratories, Campbell, CA; provided with the Hooke KitTM for EAE induction). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Immunohistochemistry:

… Subunidade beta da toxina colerica List biological, goat …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)