Bacterial Toxin Research Citations

Oral DMF Gavage and EAE Induction

… Induction of active EAE was performed as previously described (38, 39). For DC transfer experiments, 1 × 105 DC, cultured in vitro for 10 days with or without 200 µM MMF (Sigma-Aldrich) and stimulated with 1 µg/ml lipopolysaccharide (LPS) (Sigma-Aldrich) for 24 h, were injected intraperitoneally at d2 and d7 after EAE induction together with 200 ng pertussis toxin (List Biological Laboratories).

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Chemicals and Kits:

… Lipopolysaccharide from Eschericha coli 055:B5 List Biological Labs, Incorporated …

Spin trapping coupled to EPR:
 
… This technique was used in this project to evaluate the ROS species release from cells after stimulation by LPS, and if this environment was modified by treatment with BSO. BMPO (5-tert-butoxycarbonyl 5-methyl-1-pyrroline N-oxide) was used as a spin trap. It specifically reacts with O2.-  forming a superoxide adduct, BMPO-OOH, or with HO. forming the hydroxyl adduct, BMPO-OH which is characterised by a 4 lines absorbance spectrum (Figure 2.2). …

Cell preparation:

RAW Cells were plated at 5x106cells in 5ml of RPMI media supplemented with 10%FCS and 1%P/S into 25cm2 flasks. At 70% confluence, 120M of BSO, an inhibitor of the synthesis of GSH, was added to the culture for overnight incubation. The next day, cells were stimulated for 2h with LPS. In total four experimental conditions were tested: control, LPS, BSO, BSO+LPS.  

NOTE:  At the time this paper was written, Product #203 (5mg – LPS from Escherichia coli O55:B5) was utilized.

Product #203 (5 mg – LPS from Escherichia coli O55:B5) is no longer sold.

Product #203A (2.5 mg – LPS from Escherichia coli O55:B5) is available for purchase.

• Product #203A – LPS from Escherichia coli O55:B5

Briefly, heat-extracted CFA/I, 389 CS1, CS2, CS3, CS4 or CS5 fimbriae (200 ng per well) and LT (List Biological Laboratories, Inc., Campbell, CA; 100 ng per well) were coated to wells of 2HB plates …

Lethal Toxin (LT) consists of LF + PA:

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

LPS administration:

The TLR4 ligand LPS (E. coli 055:B5, Catalog no. 423, Lot no. 4231A1) was purchased from List Laboratories. Two doses of LPS (5 and 10 mg/kg, in sterile saline) were used to test the induction of white matter injury in WT mice as described in the Results. Saline-injected mice served as controls. For induction of sepsis, LPS doses were injected subcutaneously between the shoulders of PND 2 mice as previously described [9].

• Product #423 – ULTRA PURE LPS from Escherichia coli O55:B5

MATERIALS AND METHODS:

Reagents LPS and poly[I:C] was purchased from List Biological Laboratories (San Jose, CA) and Amersham Biosciences (Piscataway, NJ), respectively. …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

EAE:

Briefly, mice were immunized subcutaneously with 200 µg mouse MOG35-55 peptide (Stanford PAN facility) emulsified in CFA (200 µg M. tuberculosis, Difco adjuvants, BD) and injected intravenously with 100 ng pertussis toxin (List Biological Laboratories) at 0h and 48h of immunization.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Quantification of anti-PA IgG antibodies:

A 96-well plate (Immulon 2 HB Southern Biological, Birmingham, AL, USA) was coated with 100 l of rPA coating solution (1 g/ml in PBS, List Biological Laboratories, Inc. Campbell, CA, USA). …

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

… CT adjuvanted vaccine was prepared by mixing CT (List Biological), reconstituted according to manufacturer’s instructions, with C. perfringens broth, by vortexing for 2 min, to provide 0.025 µg CT/g body weight. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Briefly, mice (n=5/group) were immunized intraperitoneally with 100 µg OVA (Grade V; Sigma-Aldrich, St Louis, MO), 100 ng of B pertussis toxin (List Biologicals, Campbell, CA), and 50 µL Imject Alum (Thermo Scientific, Waltham, MA) in PBS in a total volume of 100 µL on days 0 and 14. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… After overnight incubation in serum-free medium with vehicle or 200 ng/ml pertussis toxin (List Biological Labs), …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)