Bacterial Toxin Research Citations

… Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free) List Labs Cat# 181 …

α-synuclein immunization booster

To determine immune memory of the α-synuclein immunization, we performed booster injections in two different batches (males and females) which displayed the same results. As customary to standard immunization procedures, booster injections of the α-syn61-75 were repeated halfway through the disease course (i.e., 4 weeks post immunization, 4 wpi) at the same dose and concentration in Incomplete Freud’s Adjuvant (IFA, Sigma-Aldrich) that lacks bacillus. Mice did not receive additional injections of pertussis toxin to prevent hypersensitivity reactions. Sham mice received IFA alone. These mice were additionally bled one week after receiving booster injections.

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

2.18 EAE induction
Chronic EAE was induced as described (Crooks et al., 2017). C57BL/6 female mice received a subcutaneous (sc) immunization with 200 μg of myelin oligodendrocyte glycoprotein fragment MEVGWYRSPFSRVVHLYRNGK (MOG35−55 peptide; Cambridge Research Biochemicals) in incomplete Freund’s adjuvant (Difco Laboratories), containing 4 mg/mL Mycobacterium tuberculosis TB H37 Ra (Difco Laboratories). Two hundred nanograms of pertussis toxin (List Biological Laboratories, Inc) in 200 μL PBS was injected intraperitoneally (i.p.) on the day of immunization and 2 days later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

2.1.Cell Culture Method

… For M1 polarized cells, on day 3, PMA media is removed, and cells are washed with PBS. Thereafter, polarizing media consisting of complete media with LPS (List Biological Laboratories, 421, 100 ng / mL ) …

• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4

Proteolipid Protein Peptide (Plp)-Induced EAE Model

Under anesthesia with isoflurane, female SJL/J mice were immunized in the rear flanks by subcutaneous injection (s.c.) of 100 µg PLP139 − 151 peptide (Peptides International, Zurich, Switzerland) emulsified 1:1 in Complete Freund’s Adjuvant (CFA) containing 4 mg/mL Mycobacterium tuberculosis (Chondrex, Redmond, WA, USA). On days 0 and 2 postimmunization, mice were injected intraperitoneally (i.p.) with 100 ng of pertussis toxin (List Biological Laboratories, Campbell, CA, USA). Control mice used as benchmark for histology were injected s.c. with phosphate buffer saline (PBS) emulsified 1:1 in incomplete Freund’s Adjuvant (IFA) (Sigma-Aldrich) on day 0. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

2.2 Protocol development steps

… The prediluted WB was distributed over 48 well tissue culture plates and stimulated with purified genetically detoxified pertussis toxin (PT, 5 μg/ml, heat inactivated at 80 °C for 10 minutes, LIST Biological Laboratories Inc, Campbell, CA, USA), …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Cre-dependent viral tracing. To confirm or refine the stereotaxic coordinates used to deliver AAV into each brain region (described
below) and to directly visualize the tissue area covered by 500 nl injection volumes, we conducted pilot studies using the surgical, perfusion,
and immunohistochemical approaches described below. Rats received 500 nl of either Fluoro-Gold (0.05%; Fluorochrome) or cholera toxin
beta (CTb; 0.05–0.1%; List Biological Laboratories #104) into each targeted brain region and were perfused on the same day or the following day. Brains were processed for either direct visualization (i.e., FG immunofluorescence) or immunocytochemical localization of CTb (immunolabeling described below) to reveal injection sites. In other rats, CTb (0.01%) was mixed with retrograde AAV (AAVrg), and CTb immunolabeling used to identify injection sites
3 weeks later. …

Immunocytochemistry … Sections from rats that received a single-site AAV injection were double-labeled for EGFP and RFP or for EGFP and DBH. In rats that received dual PVH/vlBST injections (in which CTb was included in the AAVrg mixture to mark vlBST injection sites), one set of tissue was processed for triple immunofluorescent localization of CTb (goat anti-CTb; 1:5 K), …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt
• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Animals and Genotyping

In this paper, the different mice including wild-type C57BL/6J mice, transgene Lgr5-EGFPcreERT2 mice (Jackson Laboratory), Sox9 creERT2 mice (Jackson Laboratory), and Rosa26-CAG-tdTomato mice (Jackson Laboratory) were used. In P1-2 mice, the Cre recombination enzyme was activated by intraperitoneal injection of tamoxifen (Sigma, T5648), and the dose was controlled at 0.3 mg/4 g. Diptheria toxin (List Biologicals Lab) was intraperitoneally injected into P4 mice at a dose of 10 ng g−1. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

…. Pertussis toxin List Biological Labs 180 …

EAE induction

Foxp3YFPCreMll1fl/fl,Foxp3YFPCreMll1fl/+ and Foxp3YFPCre mice (with inguinal and axillary lymph nodes on the back) were injected subcutaneously on day 0 with 200 mg MOG35-55 emulsified in 800 mg Complete Freund’s adjuvant (CFA), and injected Intraperitoneally with 500 ng of inactivated pertussis toxin on day 0 and 2. …

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer

2.2.1. Experimental Autoimmune Uveitis Induction

To evaluate the therapeutic effect of Clarstatin, we performed experiments on C57BL/6J mice in an experimental autoimmune uveitis (EAU) mice model. … The mixture contained 2.5 mg/mL Mycobacterium tuberculosis H37RA (BD, Bethesda, MD, USA). An additional intraperitoneal injection of 1 µg of purified Bordetella pertussis toxin (PTX) (List biological laboratories, Campbell, CA, USA) was also applied to each animal. The control mice were immunized with the same volume of PBS instead of IRBP in CFA and PTX. The mice were sacrificed at day 36 after primary immunization.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Experimental autoimmune uveitis induction and assessment

EAU was induced in mice using a protocol described previously [24] with minor modifications. Briefly, anesthetized 8-week-old C57BL/6 mice (50 mg/kg sodium pentobarbital, Tocris, 4579) were immunized with 200 µg human IRBP peptide 651–670 (LAQGAYRTAVDLESLASQLT, Shanghai Hanhong, CSP0725) which was emulsified in Complete Freund’s Adjuvant (1:1 vol/vol, CFA, Sigma, F58881) containing an additional 3.5 mg/mL Mycobacterium tuberculosis H37Ra (BD, 231,141), by injection with a 30G needle into the two thighs (50 µg each) and the tail base (100 µg). Concurrent with immunization, 1 µg of pertussis toxin (PTX, List Biological, 180) was injected intraperitoneally (recorded as day 0), followed by another injection of 1 µg PTX at 2 d.p.i. …

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer