Citations

… CTB (Choleragenoid, 103B) and CT (100B) were purchased from List Biological Laboratories. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae
• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… At the times of immunization and 2 d later, mice were iv injected with 400 ng of pertussis toxin (List Biological Laboratories). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

OVA/cholera toxin allergy model

OVA grade III (A5378; Sigma-Aldrich) was provided at 0.1% in drinking water, autoclaved for sterility for 3 d (Feeding, Tolerance) or not (Allergy) to initialize tolerance. All mice were then provided with regular drinking water for 1 wk. 1 mg OVA in 0.2 M sodium bicarbonate (Feeding) or 1 mg OVA + 20 µg cholera toxin (100B; List Biological) in 0.2 M sodium bicarbonate (Tolerance, Allergy) were provided once per week for 3 wk, followed by endpoint analysis 2 d after the final dose. Serum was harvested for ELISA 1 d prior to endpoint analysis. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… Pertussis toxin (180) was purchased from List Biological Laboratories (Campbell, CA, US). …

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer

2.1. Bacterial Strains, Reagents, Growth Conditions, and Antibiotic Susceptibility

… The presence of Stx2 (List Labs, Campbell, CA, USA) was determined by Western slot-blot analysis of filtered culture supernates with an anti-Stx2 monoclonal antibody [57] that was obtained from Dr. Alison D. O’Brian (Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences, Bethesda, MD, USA). The results of these studies and other characteristics [58,59,60] are presented in Table 1.

• Product #162L – Shiga Toxin 2 from Escherichia coli

… Pertussis toxin (PTX) was purchased from List Biological Laboratories, Inc. (Campbell, California). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… For expression analysis, cells were harvested in 0.5 mL of RNAlater™ (Invitrogen™) and stored at -80 °C until analyzed. Escherichia coli LPS (O55:B5; List Biologicals, Campbell, CA, USA) was purchased from List Biologicals (Campbell, CA, USA) and diluted in RPMI culture media …

• Product #203A – LPS from Escherichia coli O55:B5

… complete Freund’s adjuvant (Sigma Co, USA) and Bordetella pertussis toxin (List Biological Lab, USA) were produced for EAE induction. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

2.3.2. Lipopolysaccharide preparation and administration

LPS doses were prepared in the UAB CRU using an aseptic, no-touch technique. LPS (E. coli Type 0113 Clinical Center Reference Endotoxin) was manufactured by List Biological Laboratories, Inc. The Lot #94332B1 vials containing 1.0 mcg endotoxin were reconstituted to 1.0mcg/1.0 mL, using sterile water. 0.5 mL was drawn and diluted with 9.5 mL of sterile water to produce a 50 ng/mL solution for dosing. Dosages were drawn at 0.4 ng/kg (FM N = 7; HC N = 7) or 0.3 ng/kg (FM N = 5; HC N = 6) body weight. Finally, the dose was diluted in 30 mL sterile saline for administration.

• Product #9433 – GMP LPS Lipopolysaccharide List™ Hpt™ From Escherichia Coli Type O113