Product # 532 is MAPKKide® Plus Specific Substrate for Anthrax Lethal Factor. This peptide is remarkable in its resistance to non-specific cleavage by enzymes which may be present in natural samples. MAPKKide® Plus carries the fluorophore 7-amido-4-methylcoumarin (AMC).
Lethal Factor (LF) is the enzymatic subunit of anthrax lethal toxin. When intoxicating a cell, LF binds to PA on the cell surface and translocates through a pore made by seven PA monomers. List Labs offers several forms of LF, all made recombinantly in the native Bacillus anthracis host.
Product #169 (LF-A), is native LF, recommended for most users and is provided in a liquid form;
Product #172 (LF-HMA), the original LF made by List Labs, has been found to carry two additional amino acids, histidine and methionine, on the N-terminal. This product is recommended only for use in assays validated with this product; and finally
Product #176 (LF E687C) is an enzymatic inactive form of LF, carrying a Glu to Cys substitution in the zinc-binding site.
LF Substrates and Antibodies
List Labs has designed substrates available as MAPKKide® which mimic the native targets for LF, proteins in the MAPKK family. These FRET peptides contain the cleavage site for LF and fluorophores which are quenched based on fluorescence resonance energy transfer (FRET). Cleavage of each substrate by LF releases the fluorophore creating an increase in fluorescent intensity. To follow are available LF peptide substrates carrying three different fluorophores:
Product #532 is MAPKKide® Plus Specific Substrate for Anthrax Lethal Factor. This peptide is remarkable in its resistance to non-specific cleavage by enzymes which may be present in natural samples. MAPKKide® Plus carries the fluorophore 7-amido-4-methylcoumarin (AMC).
Product #530 is MAPKKide® utilizing the o-Abz/Dnp FRET pair. A calibration peptide, Product #539 is available for convenient use with the quenched substrate.
Product #531 is MAPKKide® with the FRET pair DABCYL/FITC.
List offers pair of antibodies directed to LF, suitable for use in and ELISA.
Product #768L affinity purified and biotinylated Anti-Lethal Factor from B. anthracis (chicken lgY) works to detect the LF as described in the C of A.
Product #769B Anti-Lethal Factor from B. anthracis (chicken lgY) may be used to capture LF. Additionally, we have demonstrated that the antibody #769B may be used to capture LF and use the MAPKKide®substrate.
Anthrax toxin can enter living cells and the toxin enzymes, Lethal Factor (LF), and Edema Factor (EF) make known changes. Because of this activity, anthrax toxins are valuable tools to investigate cell processes. Some of the work currently accomplished with these toxins can be described by the following selected references: Work with anthrax toxins is […]
A fast, sensitive, specific and accurate detection method to determine active infection Dr. Nancy Shine (408) 874-1305 NShine@ListLabs.com [Campbell, CA, 11/29/2018] • Method to detect Anthrax before it’s deadly • Anthrax is a problem for livestock • Test method is both specific and sensitive for Anthrax • List Labs is looking to partner on this […]
By: Rachel Berlin, Marketing Manager Microbiome research is uncovering the enormous potential for developing drugs, such a live biotherapeutic products, from the microbiome. This burgeoning field is the future of medicine. List Labs is excited and proud to support microbiome research by providing reagents to scientists studying the human microbiome. Below is a list of microbiome […]
By: Nancy Shine, PhD, Director of R&D, List Labs A fast, sensitive, specific and accurate detection method to determine active infection by Bacillus anthracis in plasma has been developed at List Biological Laboratories. Bacillus anthracis is regarded as a major biological warfare threat. The inhalation form of Bacillus anthracis infection can kill quickly. While antibiotic […]
By: Rachel Berlin, Marketing Manager List Labs is proud to be exhibiting at ASM Biothreats February 12-14th. The conference will be held in Baltimore, Maryland at the Hilton Hotel. Thought leaders in academia, industry and government will gather to present and discuss the latest developments in the emerging field of biothreats. This year’s conference has […]
Over 20 Scientific Posters Available on our website Scientific posters are a great way to visually display complex scientific issues. List Labs has a large list of scientific posters published on our website under the specific products they pertain to. We have been getting a lot of requests lately to compile a list of all […]
By: Nancy Shine, Ph.D., Director of Research & Development List Biological Laboratories, Inc. has designed a fluorescently labeled substrate for specific and quantitative detection of anthrax lethal factor in plasma. Bacillus anthracis is regarded as a major biological warfare threat. The inhalation form of Bacillus anthracis infection can kill quickly. While antibiotic treatment can clear […]
By: Suzanne Canada, Ph.D. Tanager Medical Writing An exciting report was released in October about a new class of targeted anti-tumor drugs, in which genetically engineered stem cells were used to deliver cytotoxins to brain tumors.1 Brain cancers known as glioblastomas (GBM) are notoriously difficult to treat because the tumors often re-grow after surgery and because […]
By: Suzanne Canada, Ph.D. Tanager Medical Writing Three anthrax toxin components—Protective antigen (PA), edema factor (EF) and lethal factor (LF) are available for research purposes from LIST Biological Laboratories, separately at a high level of purification. At least two out of three of these components are necessary to enter a mammalian cell and exert […]
By: Suzanne Canada, Ph.D. Tanager Medical Writing Vaccines have been used to help control diseases for more than 200 years and are the common practice for children and adults. Childhood vaccination has substantially reduced the morbidity and mortality from infectious diseases in much of the developed world, and influenza vaccinations have reduced the impact […]
What Are Toxin Neutralization Assays & How Do They Work? For clinical detection or vaccine testing, it is hard to beat a toxin neutralization assay. Toxin neutralization assays (TNA) assess the ability of antibodies to protect cells in culture from the cytotoxic affect of the specific toxins. Interestingly, these assays may be used for sensitive […]