Bacterial Toxin Research Citations

EAE:

… At 0 and 48 h after immunization, the mice were intraperitoneally injected with 400 ng/mouse of pertussis toxin (List Biological Laboratories, Inc., Campbell, CA, USA). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Abstract – …A fluorescent tag-labeled synthetic peptide (SNAPtide) derived from a segment of SNAP-25, an intracellular substrate of BoNT/A, is used to detect and assay the endopeptidase activity of BoNT/A.

List Biologicals developed a 13-mer peptide by replacing the Glutamine-194 of SNAP-25 with glutamic acid SNAPtide, which showed catalytic efficiency (Kcat/Km) of LCA as 28.5 10 4 M 1 sec 1 (Todd et al., 2005), which is comparable to full-length substrate GST 

List Labs provides the following SNAPtide products:

• Product #520 – SNAPtide Peptide Substrate (o-Abz/Dnp) for C. botulinum Type A Neurotoxin
• Product #521 – SNAPtide Peptide Substrate (FITC/DABCYL) for C. botulinum Type A Neurotoxin

Heptanol abrasions & tracer applications:

… One week after abrasion, 1% Cholera toxin B (CTb; List Laboratories, Campbell, CA) was applied to the cornea using a similar method, but with a smaller metal ring (7-gauge (3.8 mm ID), HTX-07R, Small Parts, Inc.).  For both tract tracer applications, the dye was left on the cornea for 30 peer-reviewed) is the author/funder. All rights reserved. No reuse allowed without permission. The copyright holder for this preprint (which was not. http://dx.doi.org/10.1101/242685doi: bioRxiv preprint first posted online Jan. 4, 2018;
5 minutes [22].  Animals were perfused 1 week after CTb application as described below and trigeminal ganglia
(TG) were examined for the presence of single (CTb)- or dual (CTb and FG)-labeled neurons (see section 2.5).

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Treatment with inflammatory inducers:

The cells were incubated 3 to 7 days in monolayer culture and were then stimulated with recombinant IL-1 (5 ng/mL, R&D Systems) or LPS (10 ng/mL, Escherichia coli 055:B5, List Biological Laboratories, CA, USA) for 24 h. Unstimulated cells were used as controls.

NOTE:  At the time this paper was written, Product #203 (5mg – LPS from Escherichia coli O55:B5) was utilized.

Product #203 (5 mg – LPS from Escherichia coli O55:B5) is no longer sold.

Product #203A (2.5 mg – LPS from Escherichia coli O55:B5) is available for purchase.

• Product #203A – LPS from Escherichia coli O55:B5

… Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native, List Biological Laboratories, Cat#169A. Anthrax Protective Antigen (PA), Recombinant from B. anthracis, List Biological Laboratories, Cat#171E. …

• Product #171E – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

List Labs Product #169A (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence; Lyophilized) has been discontinued; however, Product #169L (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence; Liquid) is available for purchase.

• Product #169L – Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence

… Pertussis Toxin (PTX) (List Biological Labs, Campbell, CA, USA), …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… To enhance responsiveness, mice received an intraperitoneal injection of 500 ng pertussis toxin (List Biological Laboratories, Campbell, CA) on days 0 and 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Under visual guidance, viral solution containing the French subtype of CVS-11 strain of RABV … in conjunction with cholera toxin -subunit (CTb, low salt; List Biological Laboratories, 1% w/v in 0.2 M phosphate buffer (PB) …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Mice were injected intraperitoneally with 300 ng pertussis toxin (List Biological) on days 0 and 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Materials:

LPS from Salmonella typhimuirum (no. 225) was purchased from List Biological Laboratories (Campbell, CA). …

Cell culture and stimulation:

…  LPS (100 ng/ml) was added to cells during both the overnight pulse and chase period.  Lysosomal damage was induced by feeding BMM 3 m AW beads in RPMI lacking serum for 60 min.  AW beads were added at a
concentration empirically determined to result in uptake of on average 3 to 4 beads per cell by
both resting and LPS-activated BMM.  All analyses of damage were performed on cells that had
internalized 3 to 7 beads per cell. …

• Product #225 – LPS from Salmonella typhimurium