Bacterial Toxin Research Citations

… sensitization was achieved by gastric gavage of PN (1 mg) and cholera toxin (CT, 10 µg, Cat. #100B List Biologicals; Campbell, CA) in 200 µl of USP saline on days 1, 8, 15 and 22. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

EAE:

Mice received intraperitoneal injections of pertussis toxin (Ptx, List Biologicals, Campbell, CA) on the day of immunization (75 ng) and 2 days later (200 ng). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Labeling and quantification of RGCs:

… Chorea toxin subunit B(CTB) (List Biological Laboratories, USA) was used as a retrograde tracer to label RGCs, which was performed as previously described in detail [13]. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

MATERIALS AND METHODS:

Reagents – LPS from E. coli serotype 0111:B4 was purchased from List Biological Laboratories (Campbell, CA). …

• Product #201 – LPS from Escherichia coli O111:B4

… Cells were stimulated with 100 ng/ml ultrapure LPS (List Biological Laboratories, Campbell, CA) for 4 or 16 h, depending on the experiment, …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

Mice, Cells, and Reagents:

… Salmonella minnesota ultra pure lipopolysaccharide (LPS) was from List Biologicals Laboratories (Campbell, CA), …

Results – SIRT2 Deficiency Does Not Affect Cytokine Response of BMDMs Exposed to Microbial Ligands and Sensitivity of Mice to Endotoxemia:

… To assess the relevance of these observations in vivo, we developed models of endotoxemia of different severity (Figures (Figures5AD).5AD). In a mild model of endotoxemia (induced by an i.p. challenge with 10mg/kg LPS), TNF and IL-12p40 concentrations in blood and mortality rates (83 vs 100%, P=0.3) were comparable in SIRT2^+/+ and SIRT2^/ mice (Figures (Figures5A,B).5A,B). In a severe model of endotoxemia (induced by 25mg/kg LPS), TNF, IL-6, and IL-12p40 concentrations in blood and mortality rates …

• Product #434 – ULTRA PURE LPS from Salmonella minnesota R595 (Re)

Cell culture:

Mouse lung epithelial cells (MLE-12) were cultured in DMEM/F-12 medium (ATCC) supplemented with 5 g/ml insulin, 10 g/ml transferrin, 30 nM sodium selenite, 10 nM hydrocortisone, 10 nM beta-estradiol, 2 mM L-glutamine, 10 mM HEPES, and 10% fetal bovine serum (Sigma-Aldrich, St. Louis, MO). Cells were cultured at 37C in 5% CO₂ and were subcultured continuously (2/wk) for a maximum of 32 sub-passages. In some experiments, LPS (100 ng/ml) was added to serum free medium (12 h). Ultrapure LPS (Escherichia coli 0111:B4) was from List Biological Laboratories (Vandell way, CA) and is reported to be free of contaminating proteins and to selectively activate TLR4 [25]. …

• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4

Animal Experimentation:

… For EAE induction, 1012-week-old mice were anesthetized and subcutaneously injected with 200 µg MOG₃₅₅₅ and 200 µg Complete Freunds Adjuvant (CFA), containing 4 mg/ml Mycobacterium tuberculosis (H37RA, BD Biosciences). Pertussis toxin (200 ng/mouse, List Biological Laboratories, Campbell, CA) was applied intraperitoneally on days 0 and 2 post immunization (p.i.). Daily clinical evaluation was performed via a 5-point scale. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Cells and Reagents:

… Lyophilized recombinant PA and LF were purchased from List Biological Laboratories, Inc. (Campbell, CA), and reconstituted in sterile water to make stock solutions with final concentrations of 1 mg/mL. …

Cell Proliferation Analysis:

Eighty thousand Hepa1c1c7 cells were added into individual wells of 12-well plates. Six replicates were used for each group. The cells were treated with or without LT for 48 h. The cell number in each well was counted after the treatment using a TC20 automated cell counter (BioRad, Hercules, CA). LT-induced cell proliferation inhibition was calculated using the following formula: …

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

… Tracing To identify SNpc neurons projecting to the DVC, either the retrograde tracer cholera toxin subunit B (CTB, 0.5% w/v, List Biological Laboratories, Campbell, CA) or the anterograde tracer … goat–CTB (List Biological Labs, …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt
• Product #703 – Anti-Cholera Toxin B Subunit (Goat)