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May 12, 2024
Toxins
Martin, V;Carre, D;Bilbault, H;Oster, S;Limana, L;Sebal, F;Favre-Guilmard, C;Kalinichev, M;Leveque, C;Boulifard, V;George, C;Lezmi, S;
Product: Botulinum Neurotoxin Type E, Nicked, from Clostridium botulinum
5.2. BoNT Administration Research-grade, purified, native BoNT serotypes E and A (BoNT/E, BoNT/A; 150 kDa) were purchased from List Labs, Campbell, California, USA (#141A and #130B, respectively). BoNT/E and BoNT/A were reconstituted in 1 mg/mL bovine serum albumin in Dulbecco’s phosphate-buffered saline …
May 10, 2024
Research Square
Jank, L;Kesharwani, A;Ryu, T;Joshi, D;Ladakis, D;Smith, M;Singh, S;Arab, T;Witwer, K;Calabresi, P;Na, C;Bhargava, P;
Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer
Induction of EAE and clinical scoring
EAE was induced in 10-13-week-old female C57BL/6J mice as previously described [21]. In brief, to induce EAE, we subcutaneously injected 150 µg of MOG33 − 55 (obtained from Johns Hopkins Peptide Synthesis Core) along with complete Freund’s adjuvant (CFA) (ThermoScientific, Rockford, IL, USA) containing 600µg of mycobacterium tuberculosis (BD, Franklin Lakes, NJ) in the lateral abdomen, followed by intraperitoneal injection with 300ng of pertussis toxin (List Biological Labs, Campbell, California, USA) on the same day of immunization and after 2 days. …
Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)
May 10, 2024
SSRN
Wuerch, E;Mirzaei, R;Yong, V;
Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer
2.4 EAE immunization and scoring
EAE was induced via subcutaneous immunization at each hind flank with 50 μg of myelin oligodendrocyte glycoprotein (MOG35–55; Protein and Nucleic Acid Facility, Stanford University) emulsified in complete Freund’s adjuvant (CFA; Fisher Scientific) and supplemented with mycobacterium tuberculosis (Sigma) on day 0. Pertussis toxin (List Biological Laboratories) was administered via intraperitoneal injection at a dose of 300 ng/200 μl on days 0 and 2. …
Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)
May 9, 2024
Cell
Liu, X;Xin, DE;Zhong, X;Zhao, C;Li, Z;Zhang, L;Dourson, AJ;Lee, L;Mishra, S;Bayat, AE;Nicholson, E;Seibel, WL;Yan, B;Mason, J;Turner, BJ;Gonsalvez, DG;Ong, W;Chew, SY;Ghosh, B;Yoon, SO;Xin, M;He, Z;Tchieu, J;Wegner, M;Nave, KA;Franklin, RJM;Dutta, R;Trapp, BD;Hu, M;Smith, MA;Jankowski, MP;Barton, SK;He, X;Lu, QR;
Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer
… Pertussis toxin List Biological Laboratories …
Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)
May 8, 2024
Biorxiv : The Preprint Server For Biology
Korkutata, M;De Luca, R;Fitzgerald, B;Arrigoni, E;Scammell, TE;
Product: Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt
2.3 Retrograde tracing with CTb
To retrogradely label inputs to the lateral PB area, we unilaterally injected the lateral PB (Anterior-Posterior (AP): -5.3 mm, Medial-Lateral (ML): -1.4 mm, Dorsal-Ventral (DV): 3.4 mm) of C57BL/6J mice (n=4) with 3 nL of CTb (List Biological Labs, Cat#103A, Campbell, CA; 1% in saline). To determine the neurochemical identity of lateral PB-projecting neurons, we similarly microinjected 3 nL CTb into the lateral PB of vGAT- and vGLUT2-reporter mice (n=4, each). We perfused mice one week later for histology.
• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt
May 4, 2024
Journal Of Neurochemistry
Groh, AMR;Caporicci-Dinucci, N;Afanasiev, E;Bigotte, M;Lu, B;Gertsvolf, J;Smith, MD;Garton, T;Callahan-Martin, L;Allot, A;Hatrock, DJ;Mamane, V;Drake, S;Tai, H;Ding, J;Fournier, AE;Larochelle, C;Calabresi, PA;Stratton, JA;
Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer
2.1 Animals
… Twelve-week-old C3 tdTomato reporter mice were immunized with 150 μL MOG 35-55 emulsion, which was prepared by mixing 2 mg/mL MOG 35-55 (Johns Hopkins Synthesis and Sequencing Core) 1:1 with complete Freund’s adjuvant (8 mg/mL M Tuberculosis H-37Ra [cat# 231141; BD] in Freund’s incomplete adjuvant [cat# PI-77145; Thermo Fisher]) subcutaneously on the abdomen and then injected with 250 ng of pertussis toxin (cat# NC9675592; List Biological Laboratories) intraperitoneally on the day of immunization and again 2 days later. …
Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)
May 4, 2024
Cell Discovery
Wang, Q;Ma, J;Gong, Y;Zhu, L;Tang, H;Ye, X;Su, G;Huang, F;Tan, S;Zuo, X;Gao, Y;Yang, P;
Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer
EAU induction
Six-week-old female and male C57BL/6 J, Anxa1fl/fl or Anxa1ΔLy6g mice were immunized with 500 μg of IRBP651-670 (Sangon Biotech) emulsified in complete Freund’s adjuvant (CFA; Sigma-Aldrich) and supplemented with 5.0 mg/mL Mycobacterium tuberculosis (MTB) strains. Intraperitoneal injection of 500 ng of pertussis toxin (List Biological) was performed at 0 h and 24 h. Splenic cells were collected from the mice at 1, 2, 3, and 4 weeks after immunization. …
Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)
May 3, 2024
Science Immunology
Li, R;Lei, Y;Rezk, A;Diego A Espinoza, ;Wang, J;Feng, H;Zhang, B;Barcelos, IP;Zhang, H;Yu, J;Huo, X;Zhu, F;Yang, C;Tang, H;Goldstein, AC;Banwell, BL;Hakonarson, H;Xu, H;Mingueneau, M;Sun, B;Li, H;Bar-Or, A;
Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer
… incomplete Freunds adjuvant supplemented with heat-inactivated Mycobacterium tuberculosis (5 mg/ml; Sigma-Aldrich). Pertussis toxin (List Biological Laboratories), 300 ng per mouse, was injected intraperitoneally twice at day 1 and day 3. …
Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)
May 3, 2024
Science Immunology
Miller-Little, WA;Chen, X;Salazar, V;Liu, C;Bulek, K;Zhou, JY;Li, X;Stüve, O;Stappenbeck, T;Dubyak, G;Zhao, J;Li, X;
Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer
… On days 0 and 2, each mouse was injected intraperitoneally with 0.2 µg of purified Bordetella pertussis toxin (List Laboratories). …
Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)
May 1, 2024
Journal Of Veterinary Science
Hong, S;Kim, J;Jung, K;Ahn, M;Moon, C;Nomura, Y;Matsuda, H;Tanaka, A;Jeong, H;Shin, T;
Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer
Animals and induction of EAE
C57BL/6J mice (male, 8–10 weeks old; DBL, Korea) were used in this study (n = 8 animals per group). …
As previously described [24, 25], EAE was induced by subcutaneous injection (into the hind flank) of 1 mg/mL of myelin oligodendrocyte glycoprotein 35–55 peptide (purity > 96.44%; GL Biochem, China) emulsified in complete Freund’s adjuvant (Difco Laboratories, USA) and supplemented with 5 mg/mL Mycobacterium tuberculosis H37Ra (Difco Laboratories), Pertussis toxin (500 ng; List Biological Laboratories, USA) was intraperitoneally injected on days 0 and 2 postimmunization (PI). …
Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)
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