Bacterial Toxin Research Citations

EAE Model:

EAE was induced by s.c. injection of 200 g of myelin oligodendrocyte glycoprotein (MOG)₃₅₅₅ (GenScript) with IFA (Difco) plus 500 g nonviable, desiccated Mycobacterium tuberculosis (Difco) into the hind flank. On the day of and 2 d after immunization, 200 ng of pertussis toxin (List Biological) was injected intraperitoneally. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Subjects and experimental outline:

In total, 40 mice (both genders) were used in this experiment with 20 each assigned to the control (Cre) and experimental (Cre⁺) groups. All subjects in both groups received one intraperitoneal injection (i.p.) of DT at a dose of 25 ng/g (List Biological Laboratories, Campbell, CA, USA) at postnatal day 21. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Toxins:

TcdA and TcdB₍₀₀₃₎ were purchased from List Biological Laboratories …

Murine passive protection:

Female C57BL/6 mice (78 weeks old) were administered 2 mg anti-T-toxin hIgG or PBS IP. Mice were challenged IP 24 h later with a MLD_100% of TcdA, TcdB₍₀₀₃₎, or CDTa plus CDTb and observed for 7 days.

• Product #152C – Toxin A from Clostridium difficile 
• Product #155 – Toxin B from Clostridium difficile

Macrophage culture and activation:

Murine peritoneal macrophages were elicited with thioglycollate (BD, Sparks, MD, USA; same batch for all strains) for 4 days. Cells from up to 4 mice were pooled and plated at 4 10⁶ cellsml¹ in DMEM+20% FBS+1% streptomycin/penicillin in duplicates or triplicates. After overnight culture, adherent cells were selected (adherence assay for macrophage enrichment). RNA deconvolution shows two main populations of inflammatory macrophages (F4/80+, MHCII and F4/80, MHCII+) and a small population of neutrophils (Supplementary Fig. 2). Cells were incubated with 2 ngml¹ LPS (List Biological, Campbell, CA, USA) or control in DMEM+1% FBS for 4h before harvest. …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

Induction and clinical assessment of EAE:

…Mice were immunized subcutaneously in both sides of the flank with 200 g MP4 in 200 l CFA. Mice received intraperitoneal injections of 200 ng pertussis toxin (List Biological Laboratories, Hornby, ONT, Canada) on the day of immunization and 48 h later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

… Mice were immunized with a MOG 35-55 kit (EK-2110; Hooke Laboratories). Each mouse was immunized with a 20-L sc injection of MOG 35-55 /CFA emulsion and an ip injection of 200 ng of pertussis toxin (List Biological Laboratories) diluted in sterile PBS (14). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE disease model:

The experimental autoimmune encephalomyelitis (EAE) model was used as previously described⁴⁵. Briefly, 7-8 week old female mice were immunized s.c. with 200g MOG₃₅₅₅ (Life Technologies) in complete Freunds adjuvant (Difco). Mice were given 400ng pertussis toxin (List Biological Labs) in 500l PBS i.p. on day 0 and 2 post-immunization. For adoptive transfer, 110⁶ WT Foxp3 (GFP⁺) iTreg cells or PD-1HKO Foxp3 (GFP⁺) iTreg cells were injected i.v. before immunization on day 0. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

…Mice were injected iv with 300 ng of reconstituted lyophilized Pertussis toxin (List Biological Laboratories) on the day of immunization and two days later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

…The MDS formulations were prepared with the following parameters: Matrix materials: poly (dl-lactide-co- glycolide) and poly (dl-lactide) Microsphere mean diameter range: 6-20 m RPA of anthrax (from List Biological Laboratories, Campbell, CA, US …

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

… Pertussis-Toxin List Biological Laboratories, Campbell, CA, USA …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)