Bacterial Toxin Research Citations

…ELISAs were performed as described [23]. Briefly, 96-well plates were coated with 1 g/well of recombinant PA, LF (List Biologicals, Campbell, CA),…

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence)

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Induction and Scoring of EAE:

EAE was induced in C57/Bl6 mice according to a previously published protocol… Control, sham-immunized mice were injected with an equal volume of phosphate buered saline (PBS) and Complete Freunds Adjuvant. All animals received 200ng pertussis toxin (List Biological, Campbell, CA, USA) in 0.1ml PBS at 0h and 48h postimmunization, administered by intraperitoneal injection. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Materials:

…Pertussis toxin (PTX) was purchased from List Biological Laboratories Inc., (Campbell, CA). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… C57BL/6 (Charles River) mice were randomly assigned to treatment groups and were treated with 40 g LPS (E. coli 0111:B4; List Biological Laboratories) in 50 l 0.9% saline or 50 l 0.9% saline as a control or 50 l hydrochloric acid (HCl) 0.1N, pH 1.2 by it instillation …

• Product #201 – LPS from Escherichia coli O111:B4

Macrophage culture:

…BMDMs were primed for 4h with 600ngml¹ E. coli 0111:B4 LPS… and then transfected with poly(dA:dT)…, lethal toxin (500ngml¹, #172D and #171D mixed at a 50/50 ratio, List Labs) or flagellin…

• Product #172 – Anthrax Lethal Factor (LF), Recombinant from B. anthracis
• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Immunization and challenge of mice:

Ninety-one 6-week-old female BALB/c mice (Charles River Laboratories, MA) were randomly divided into seven groups of 13 mice and used for immunization. In protein administration groups, mice were immunized intranasally with 10 μg of rec47 with or without 10 μg of heat-labile enterotoxin B subunit (LTB; Sigma Aldrich Co., Saint Louis, MO) from E. coli or 10 μg of cholera toxin (CT; List Biological Laboratories Inc., CA, USA) adjuvants. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

EAE:

… At the same time, mice were also administered 250 ng of pertussis toxin (List Biological Laboratories) ip and again 2 d later. Clinical disease was monitored daily and scored …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Evaluation of anti-CLEC12A antibody in EAE immunized mice:

Progressive EAE was induced in female C57BL/6 mice (68 weeks old) and the CLEC12A/ mice as described previously⁶⁰⁶². Briefly, 100L of 150g myelin oligodendrocyte glycoprotein (MOG₃₅₅₅) (PolyPeptide Laboratories) peptide emulsified in complete Freunds adjuvant (Difco) containing 4mg/ml killed Mycobacterium tuberculosis (strain H37Ra; Difco) was injected subcuataneously. Following immunization, 200ng of pertussis toxin (List Labs) was injected i.p. into mice on Day 0, followed by a 400ng pertussis toxin i.p. injection on Day 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Macrophage Culture And Activation:

…After overnight culture, cells were washed in PBS and incubated with 2 ng/ml LPS (List Biological Inc., Campbell, CA, USA) or control in DMEM + 1% FBS for 4h before harvest. Viability of cultured macrophages was determined for some strains by staining with 2 M calcein AM …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

Proteins:

…Protective antigen (PA) from Bacillus anthracis (List Biological Labs, Campbell, CA, USA) was used in the assay at a final concentration of 3 g/ml. …

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis