Bacterial Toxin Research Citations

… Briefly, after the rats were deeply anaesthetized, 1% cholera toxin B subunit (CTB, List Biological Laboratories, Campbell, CA, USA) (2 µl) was injected into the superior colliculus bilaterally (6.0 mm posterior and 2.0 mm lateral to the bregma and 4-5 mm deep from the …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… After physiological mapping was completed, the animal was returned to inhalation anesthesia. A Hamilton syringe with a 28-gauge needle was used to inject CTb [2% (vol/vol) in distilled water, 0.2 µl per injection; List Biological Laboratories, Inc.] …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Isolation and maintenance of bone marrow-derived macrophages:

…For measurements of inflammatory responses, cells were stimulated with ultrapure LPS (E. coli 0111:B4, 10 ng/mL or 0.1 ng/mL; List Biological Laboratories). …

• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4

Cellular experiments:

Human monocytes were isolated from peripheral blood of healthy volunteers by Ficoll gradient separation followed by positive selection using human anti-CD14 antibody assisted magnetic bead separation…Cells were pretreated with 4-phenylbutyrate …for 1 hour and then stimulated with LPS (List Biological) and …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

… Spin-labeled protein samples were contained in custom extruded AquaStar tubing (2 M).15 LPS from Escherichia coli O111:B4 (List Biological Laboratories, Inc, Campbell, CA) was resuspended in 50 mM NaPO4, 300 mM NaCl, pH 7 buffer at a concentration …

• Product #201 – LPS from Escherichia coli O111:B4

Experimental autoimmune myocarditis:

To induce myocarditis, male mice age 6-9 weeks were immunized on days 0 and 7 with 100 g MyHC614
629 peptide emulsified in Complete Freunds Adjuvant (Sigma-Aldrich) supplemented to 5 mg/ml with heat
killed Mycobacterium tuberculosis strain H37Ra (Difco). Mice were also administered 500 ng pertussis toxin
(List Biologicals) ip on day 0.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

In vivo injection into the superior olivary nucleus

… To target the superior olivary nucleus (SON), a glass micropipette (tip diameter 40 – 60 m) was filled with 1% cholera toxin B (CTB; List Laboratories) and advanced into the brain at a 6°–10° rotation in the rostrocaudal axis and a 4° rotation in the mediolateral axis, to a depth of 9 mm. Tracer was pressure ejected with a Picospritzer II (General Valve Corp.) using 10- to 50-ms pulses at 20 psi. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

…10 ng/ml cholera-toxin (list Biological Laboratories …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Cell culture and stimulation:

RAW264 and J774 cells were cultured in DMEM containing 10% FBS at 5% CO₂ and 37C. …Cells were stimulated with ultrapure smooth LPS from Escherichia coli O111:B4 (List Biological Laboratories, Campbell, CA) in the presence of 10 or 2% FBS, respectively, at 37C. …

• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4

LPS from Escherichia coli was purchased from List Labs, …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/