Bacterial Toxin Research Citations

…Mice received intraperitoneal injections of pertussis toxin (Ptx, List Biologicals, Campbell, CA) on the day of immunization (75 ng) and 2 days later (200 ng). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

EAE was induced in C57BL/6 mice with 100 g of MOG 3555 peptide in complete Freund adjuvant… subcutaneously and 50 ng of pertussis toxin (PTX; List Biological Lab, Campbell, CA) intravenously on day 0 and +2 postimmunization as described. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

… to induce EAE. Additionally, 200 ng of pertussis toxin (List Biological Laboratories, Campbell, CA) was administered ip at day 0 and day 2. EAE paralysis of mice was scored as previously described…

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

For the ablation experiments, bigenic Gli1CreERt2; iDTR mice received tamoxifen (3x 10mg per oral gavage) 10 days prior to irradiation and BM transplantation and were injected with diphtheria toxin dissolved in PBS (List Biological Laboratories) at a dose of 50ng/g body weight intraperitoneally (i.p.) as indicated (Figure 3B).

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Retrograde labeling of ascending projection neurons in the brainstem:

For fluorescent localization, the sections were incubated successively with 10% normal rabbit serum (NRS) in PBS-X for 30 min, 1:10000 goat anti-CTB antibody (List Biological catalog #703,…

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

IL-1 secretion from BMDMs:

BMDMs (110⁵/well) were seeded into 48-well plates (growth area per well: 0.75 cm²) and cultured overnight. Cells were then primed with 5 ng/ml of ultra-pure LPS (List Biological Laboratories, Campbell, CA). After 4 h, cells were stimulated with the indicated NPs or with 1 mM ATP…

Author has informed that the following List product was utilized:

• Product #434 – ULTRA PURE LPS from Salmonella minnesota R595 (Re)

Abstract:

…One adult monkey was analysed for retrograde CB tracer injected in C5-C8 hemispinal cord to visualise reticulospinal neurons. The corticobulbar axons formed bilateral terminal fields with boutons terminaux and en passant, which were quantified in various nuclei belonging to the Ponto-Medullary Reticular Formation (PMRF). The corticobulbar projections from both PM and SMA tended to end mainly ipsilaterally in PMRF, but contralaterally when originating from M1. Furthermore, the corticobulbar projection was less dense when originating from M1 than from non-primary motor areas (PM, SMA). The main nuclei of bouton terminals corresponded to the regions where reticulospinal neurons were located with CB retrograde tracing. In conclusion, the corticobulbar projection differs according to the motor cortical area of origin in density and laterality.

Methods:

…In SC-1 the retrograde tracer cholera toxin B subunit (CB; List Biological Laboratories, Campbell, Calif, USA; 0.05% solution in distilled water) was injected unilaterally under deep anaesthesia in the cervical spinal cord at multiple sites between C5 …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Cholera toxin subunit B Purified toxin subunit (Choleragenoid) List Biological Labs (Campbell, CA), goat polyclonal, #703 …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

…Pertussis toxin (PTx), lyophilized in pure water, salt-free (List Biological Laboratories, Campbell, CA; Cat. No. 181)…

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

EAE mouse model of optic neuritis:

All experiments were performed on 10-week-old female C57BL/6 mice…Mice further received 300 ng intravenous adjuvant pertussis toxin (PTX, List Laboratories, Campbell, CA) on the day of and 2 days after immunization. Eight mice were studied.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)