Citations

Materials:

PT and the purified PTS1 subunit were purchased from List Biological Laboratories (Campbell, CA).

Toxin detection and quantification by SPR:

Wild-type or mutant CHO cells were seeded into 6-well plates in order to achieve 80% confluence after an overnight incubation. The cells were then placed at 4°C for 30 min in 1 ml serum-free medium containing 1 µg of PT. …

Intoxication assay

Wild-type or mutant CHO cells were seeded into 6-well plates and grown overnight to 80% confluence. After a 30-min incubation at 4°C in 1 ml of Ham’s F-12 medium containing 250 ng PT, the cells were washed with PBS and returned to 37°C in serum- and toxin-free medium for 3 h. …

Confocal microscopy:

CHO cells seeded onto glass coverslips in a 24-well plate were incubated with 0.5 µg/ml of PT for 30 min at 4°C. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

To induce myocarditis, male mice age 69 weeks were immunized on days 0 and 7 with 100 µg MyHC614-629 peptide emulsified in Complete Freund’s Adjuvant (Sigma-Aldrich) supplemented to 5 mg/ml with heat-killed Mycobacterium tuberculosis strain H37Ra (Difco). Mice were also administered 500 ng pertussis toxin (List Biologicals) ip on day 0.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

…In addition, each animal received pertussis toxin (PT; List Biological Laboratories, Campbell, CA; 100 ng per mouse) ip, on days 0 and 2 after the first immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

– … We gave 49 postnatal day (P)2 mice a systemic injection of diphtheria toxin (DT; 4 ng/g im List Biological Laboratories, Inc.) (Figure 2.1). Pups were raised with their mothers until weaning at P21. After weaning, pups were group housed in same sex, mixed genotype …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Lymphocyte stimulation:

…Positive control samples were stimulated using Staphylococcal Enterotoxin B (List Biological Laboratories, Campbell, CA) at 1g/mL. …

… Glass micropipettes with inner tip diameters ranging from 12 to 20 m were selected and lled with a cocktail of… and CTb (List Biological Laboratories, catalog 104) dissolved in dibasic sodium phosphate solution. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

…Each animal received bilateral injections of cholera toxin B subunit (CTB, choleragenoid, List Biological Laboratories, Campbell, USA) in SL for retrograde labeling. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

…pertussis toxin (PTX) from List Biological Laboratories (Campbell, CA, USA), and …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

CT-ELISA and -galactosidase activity assay:

…Purified CT subunit B (List Biological Laboratories, Inc., Campbell, CA) was used to provide a standard curve, and phosphate-buffered saline (PBS) was used as a negative control. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Mice, cells and reagents:

…Salmonella minnesota ultra pure lipopolysaccharide (LPS) from List Biologicals Laboratories (Campbell, CA)…

Mass cytometry (CyTOF) analysis:

Blood was collected from healthy donors in heparin tubes and stimulated with LPS (100ng/ml) with or without propionate (2mM) and brefeldin A.

• Product #434 – ULTRA PURE LPS from Salmonella minnesota R595 (Re)