Bacterial Toxin Research Citations

Retrograde labeling of ascending projection neurons in the brainstem:

For fluorescent localization, the sections were incubated successively with 10% normal rabbit serum (NRS) in PBS-X for 30 min, 1:10000 goat anti-CTB antibody (List Biological catalog #703,…

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

IL-1 secretion from BMDMs:

BMDMs (110⁵/well) were seeded into 48-well plates (growth area per well: 0.75 cm²) and cultured overnight. Cells were then primed with 5 ng/ml of ultra-pure LPS (List Biological Laboratories, Campbell, CA). After 4 h, cells were stimulated with the indicated NPs or with 1 mM ATP…

Author has informed that the following List product was utilized:

• Product #434 – ULTRA PURE LPS from Salmonella minnesota R595 (Re)

Abstract:

…One adult monkey was analysed for retrograde CB tracer injected in C5-C8 hemispinal cord to visualise reticulospinal neurons. The corticobulbar axons formed bilateral terminal fields with boutons terminaux and en passant, which were quantified in various nuclei belonging to the Ponto-Medullary Reticular Formation (PMRF). The corticobulbar projections from both PM and SMA tended to end mainly ipsilaterally in PMRF, but contralaterally when originating from M1. Furthermore, the corticobulbar projection was less dense when originating from M1 than from non-primary motor areas (PM, SMA). The main nuclei of bouton terminals corresponded to the regions where reticulospinal neurons were located with CB retrograde tracing. In conclusion, the corticobulbar projection differs according to the motor cortical area of origin in density and laterality.

Methods:

…In SC-1 the retrograde tracer cholera toxin B subunit (CB; List Biological Laboratories, Campbell, Calif, USA; 0.05% solution in distilled water) was injected unilaterally under deep anaesthesia in the cervical spinal cord at multiple sites between C5 …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Cholera toxin subunit B Purified toxin subunit (Choleragenoid) List Biological Labs (Campbell, CA), goat polyclonal, #703 …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

…Pertussis toxin (PTx), lyophilized in pure water, salt-free (List Biological Laboratories, Campbell, CA; Cat. No. 181)…

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

EAE mouse model of optic neuritis:

All experiments were performed on 10-week-old female C57BL/6 mice…Mice further received 300 ng intravenous adjuvant pertussis toxin (PTX, List Laboratories, Campbell, CA) on the day of and 2 days after immunization. Eight mice were studied.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Immunization and viral challenge in mice:

Mice (n = 4/group) were immunized with PBS (in 50 μl) as a negative control, 20 μg of purified GcfAB, GcfA or GcfB proteins with 2 μg of cholera toxin (CT; List Biological Laboratories, Campbell, CA) via intranasal (IN, in a final volume of 50 μl) or sublingual (SL, in a final volume of 15 μl) routes twice, on days 0 and 18. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

EAE induction and scoring:

… C57BL/6J, Th/+ and OSE mice were immunized s.c. with 100 g recombinant rat MOG₁₁₂₅/CFA. 300 ng/mouse pertussis toxin (PTX) (List Biological Laboratories, #180) were injected i.p. at day 0 and day 2 after immunization. …

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer

For megakaryocyte depletion, mice received daily i.p. injections of 250ng diphtheria toxin (List Biological Laboratories, 150) or PBS control. Megakaryocytes depletion was confirmed by complete blood count analyses and immunofluorescence staining.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Cholera toxin production assay:

GM₁ ganglioside enzyme-linked immunosorbent CT assays (ELISAs) were performed on the supernatants of cultures grown under AKI-inducing conditions or soy LB rhamnose-inducing conditions, and the total ng of CT produced per ml of culture per OD₆₀₀ unit (ng CT ml¹ OD₆₀₀¹) was determined as previously described [31]. Purified cholera toxin B subunit (List Biological Laboratories, Campbell, CA) was used as a standard.

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt