Citations

Cell Depletion:

For in vivo cell depletion, DT (0.5 mg/mouse/dose, List Biological Laboratories) was given intraperitoneally every 3 days for four times, then mice were sacrificed on day 10. In HFD-fed animals, DT was given in the last 10 days of the indicated HFD feeding period.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Reagents:

Anthrax lethal toxin, consisting of protective antigen and lethal factor was from List Biological Laboratories.

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

EAE:

In 2D2 TCR-Tg mice, EAE was induced with 100 mg MOG3555. At the time of and 2 d after im- munization, mice were injected ip with 300 ng Bordetella pertussis toxin (List Biological Laboratories) in PBS.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

In vivo depletion of Langerin⁺ cells:

Langerin-eGFP-DTR mice were injected i.p. with DT (1 g in 200 l saline; List Biological Laboratories). 24 h after DT injection, epidermis and dermis were separated by treatment of dispase II (1 mg/ml; Gibco) overnight at 4C, and LC depletion in the epidermis was measured by FACS analysis.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… Mice were rested for two weeks before being sensitized. After the two week rest period, mice were sensitized with 2 mg peanut extract and 10 µg cholera toxin (List Biological Laboratories, Campbell, CA) by oral gavage once a week for three weeks. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

IL-1 signaling assay:

… or Bacillus anthracis lethal factor (100 ng/ml; List Biological Laboratories) was prepared with these liposomes as controls. …

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

Materials: 

…The Anthrax PA measured by circular dichroism was obtained from List Labs (Lot: 17115A6B). …

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

…the internal solution was supplemented with 2 g ml 1 pertussis toxin (Tocris) or 1.5 g ml 1 botulinum neurotoxin C light chain (List Biological Laboratories). Neurons were dialysed with toxin-containing internal solutions for 1015 min before measurements.

• Product #625A – Botulinum Neurotoxin Type C Light Chain, Recombinant

1:20,000 b Subunit of choleric toxin Vibrio cholera b subunit toxin List Biological, goat polyclonal, #703 1:10,000…

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Constructs and reagents:

… Pertussis toxin was purchased from List Biological Laboratories, Inc (Campbell, CA).

Kinetic BRET assay:

For kinetic BRET experiments, HEK 293 cells seeded in six-well plates were transfected with 25 ng mas-GRK3ct-Luc, 200 ng HA-2A-AR, 200 ng Ven-G1, 200 ng Ven-G2, and 400 ng of either Go-C351G or Gi1-C351G. HA-RGS4 or FLAG-RGS14 was also transfected in 3, 10, 30, and 100 ng amounts. For each experiment, a control using pertussis-sensitive Go or Gi1 was included to record any noise within the system. Pertussis toxin (100 ng/mL) was added at the time of transfection to limit activation of endogenous G proteins. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)