Bacterial Toxin Research Citations

…were incubated with two-fold serially diluted pig serum or colostrum samples (diluted from 1:200 to 1:25,600) to detect anti-STa IgG or IgA antibodies; wells of Immulon 2HB microtiter plates (Thermo Scientific, Rochester, NY) coated with LT [List Biological Laboratories …

Lethal Toxin consists of Lethal Factor & Protective Antigen:

Author did not indicate which specific Lethal Factor was utilized.  List Labs provides:

• Product #172 – Anthrax Lethal Factor (LF),Recombinant from B. anthracis
• Product #169 – Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence

List Labs provides the following Protective Antigen:

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

…or cholera toxin (CT; List Biological Laboratories, CA) was mixed with the vaccine antigen immediately before injection. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Induction of EAE and Administration of LH2171:

Female SJL/J mice (5 weeks old) were used to assess the immunosuppressive effect of LH2171 on EAE. …

… in addition to two intraperitoneal injections of 400 ng of pertussis toxin (List Laboratories, Campbell, CA, United States) on days 0 and 2. EAE mice were divided into two groups: …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

…in 96-well flat-bottomed tissue culture plates at 2.5×10 4 cells/well in 50 L media and incubated for 16-19 h at 37 C with 5% CO 2 . pp-dPA83 formulations were titrated on the plated cells in the presence of a constant concentration of LF (List Biological Laboratories …

Author did not specify which Lethal Factor was utilized.  List Labs provides the following Lethal Factor:

• Product #172 – Anthrax Lethal Factor (LF), Recombinant from B. anthracis
• Product #169 – Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence

Retrograde and Fos double labeling of pre-oculomotor neurons:

In the last four rats, cholera toxin B (CTB) was delivered into the III to retrogradely label contralateral INC/DN pre-oculomotor neurons. …The negative control rats suffered stereotaxic frame mounting, craniotomy and saline injection alone without down-stretching the jaw^[18]. Then, coronal frozen sections (30 mm in thickness) were cut through the midbrain and pons. The sections were incubated with rabbit anti-Fos antibody (Santa Cruz Biotech) and goat anti-CTB serum (1:500, List Biological Laboratories, USA) overnight.

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Detection of serum antibodies:

ELISA was used to detect and quantify antibodies binding Fha, LOS and PTx antigens from B. pertussis (List Labs, Campbell, CA, U.S.A.)

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer

EAE induction, prevention, and treatment:

C57BL/6 mice (812 weeks old) were immunized subcutaneously with 6 mg/mL synthetic peptide of myelin oligodendrocyte glycoprotein (MOG₃₅₅₅) peptide (MEVGWYRSPFSRVVHLYRNGK,… In addition, the animals received 250 ng pertussis toxin (List Biological Laboratories) intraperitoneally (I.P.) on days of immunization and 48 h later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

PBMC isolation and culture:

PBMC (peripheral blood mononuclear cells) were isolated from collected blood or leukapheresis by density gradient centrifugation, according to the manufacturers instructions. Cells were cryopreserved in liquid nitrogen suspended in FBS containing 10% (vol/vol) DMSO. For PBMCs in vitro expansion, 2 x 10⁶ cells/ml were cultured in the presence of 5 g/ml Tetanus Toxoid (TT) (List Biological Laboratories Inc., Campbell, CA)…

• Product #191 – Tetanus Toxoid from Clostridium tetani

…In the same buffer, 24.5 L of 6 M SNAPtide substrate (no. 521 from List Labs) was added to the enzyme + compound solution, and the plate was read for 25 min at 490 nm (ex)/523 nm (em)/495 nm (cutoff). …

• Product #521 – SNAPtide Peptide Substrate (FITC/DABCYL) for C. botulinum Type A Neurotoxin

…a membrane permeable, nonhydrolyzable cAMP analog, and TeNT (20 nM; List Biological Laboratories), which cleaves the synaptic vesicle protein synaptobrevin and prevents transmitter release…

• Product #190 – Tetanus Toxin from Clostridium tetani