Bacterial Toxin Research Citations

…Pertussis toxin (PTx), lyophilized in pure water, salt-free (List Biological Laboratories, Campbell, CA; Cat. No. 181)…

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

EAE mouse model of optic neuritis:

All experiments were performed on 10-week-old female C57BL/6 mice…Mice further received 300 ng intravenous adjuvant pertussis toxin (PTX, List Laboratories, Campbell, CA) on the day of and 2 days after immunization. Eight mice were studied.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Immunization and viral challenge in mice:

Mice (n = 4/group) were immunized with PBS (in 50 μl) as a negative control, 20 μg of purified GcfAB, GcfA or GcfB proteins with 2 μg of cholera toxin (CT; List Biological Laboratories, Campbell, CA) via intranasal (IN, in a final volume of 50 μl) or sublingual (SL, in a final volume of 15 μl) routes twice, on days 0 and 18. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

EAE induction and scoring:

… C57BL/6J, Th/+ and OSE mice were immunized s.c. with 100 g recombinant rat MOG₁₁₂₅/CFA. 300 ng/mouse pertussis toxin (PTX) (List Biological Laboratories, #180) were injected i.p. at day 0 and day 2 after immunization. …

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer

For megakaryocyte depletion, mice received daily i.p. injections of 250ng diphtheria toxin (List Biological Laboratories, 150) or PBS control. Megakaryocytes depletion was confirmed by complete blood count analyses and immunofluorescence staining.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Cholera toxin production assay:

GM₁ ganglioside enzyme-linked immunosorbent CT assays (ELISAs) were performed on the supernatants of cultures grown under AKI-inducing conditions or soy LB rhamnose-inducing conditions, and the total ng of CT produced per ml of culture per OD₆₀₀ unit (ng CT ml¹ OD₆₀₀¹) was determined as previously described [31]. Purified cholera toxin B subunit (List Biological Laboratories, Campbell, CA) was used as a standard.

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… difficile toxin B (product number #155A), Staphylococcus aureus alpha toxin (product number #120C), Staphylococcus aureus enterotoxin type B (product number #122C), Salmonella typhimurium lipopolysaccharide (product number #225, all List Biological Laboratories …

• Product #155- Toxin B from Clostridium difficile
• Product #120 – Alpha Toxin from Staphylococcus aureus
• Product #122 – Enterotoxin Type B from Staphylococcus aureus
• Product #225 – LPS from Salmonella typhimurium

To induce EAM, mice received subcutaneous immunizations on days 0 and 7 of 100 g myosin heavy chain (MyHC)614629 peptide… On day 0, mice also received 500 ng pertussis toxin intraperitoneally (List Biologicals; …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

 Analysis of serum and mucosal antibodies:
 
Serum antibodies were analyzed by ELISA. High-affinity ninety-six well plates
designed for EIA/RIA (Thermo Scientific, Waltham, MA) were coated with 0.5 g/ml of
purified C. difficile Toxin A Toxoid and Toxin B Toxoid (List Biologicals, Campbell, CA) for 24
hours. Non-specific binding was blocked by incubating coated plates with 200 l of 5%BSA in
PBS-T for at least 2 hours at room temperature. Serum, mucosal extract or supernatant from …

…Cholera toxin (List Biological Laboratory, No. 100B) ! CAUTION Cholera toxin is potentially harmful. Wear gloves and handle it carefully in a biosafety cabinet. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae