Bacterial Toxin Research Citations

Human PBMC were obtained from donor whole blood (harvested in our lab at UMass) using Lymphoprep density gradient (Axis-Shield) and stimulated in RPMI 1640 supplemented with 10% FCS and 25mM HEPES. TcdA and TcdB were from List Biological Labs.

• Product #152C – Toxin A from Clostridium difficile
• Product #155 – Toxin B from Clostridium difficile

Test substances:

The following proteins were used in this study…Different positive control substances were used depending on the variable being measured. Clostridium difficile Toxin A (2 g/ml; List Biological Laboratories, Inc., Campbell, California, USA) was used as a protein toxin positive control for barrier integrity/permeability effects;…

• Product #152C – Toxin A from Clostridium difficile

– … monoclonal anti-botulinum neurotoxin type A (731A, –BoNT-A mAb, mouse IgG), botulinum neurotoxin type A toxoid from Clostridium botulinum (133L, BoNT-A toxoid), and recombinant botulinum neurotoxin type B light chain (620A, BoNT-B), from List Biological …

• Product #731A – Anti-Botulinum Neurotoxin, Type A (Mouse lgG monoclonal F1-40)
• Product #133L – Botulinum Neurotoxin Type A Toxoid from Clostridium botulinum
• Product #620A – Botulinum Neurotoxin Type B Light Chain, Recombinant

…Cholera toxin (100) and insulin (16634) were from List Biological (Campbell, CA, USA) and Invitrogen (Carlsbad, CA, USA), respectively. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

…A primary antiserum against CT-B subunit (goat CT-B, 1:2500; List Biological Laboratories Inc., Campbell CA, USA; kind gift from Colin Anderson, University of Melbourne, VIC, Australia) diluted in PBS was applied to each section. …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

… Normal mouse EC (NEC) were originally isolated from mouse mammary glands and cultured in EGM-2 [6]. Growth factors or lipopolysaccharide (LPS, List Biological Laboratories 201) were added to cultures at indicated concentrations. …

• Product #201 – LPS from Escherichia coli O111:B4

EAE induction:

EAE was induced in C57BL/6 mice by subcutaneous challenge with 200 µg MOG₃₅₅₅ (Peptides International, Louisville, KY) in 200 µL of complete Freund’s adjuvant (Sigma-Aldrich, St. Louis, MO). On days 0 and 2 postchallenge, mice received 400 ng of Bordetella pertussis toxin intraperitoneally (List Biological Laboratories, Campbell, CA). Mice were monitored and scored daily for disease progression as previously shown.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Animals and Induction and evaluation of EAE:

EAE was induced in male and female B6 and PWD mice as follows. Mice were injected subcutaneously with 0.1 ml of emulsion containing 2.5 mg of MSCH in PBS and 50% CFA (Sigma, USA) on day 0 and day 7. CFA was supplemented with 4 mg/ml Mycobacterium tuberculosis H37Ra (Difco, USA). On day 0 and day 2 mice also received an i.p. injection of 200 ng pertussis toxin (List Laboratories, USA) as an ancillary adjuvant. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

…50 µl of the emulsion were injected in each axillary fossa. 200 ng of pertussis toxin (LIST BIOLOGICAL LABORATORIES, INC.) were administered intravenously at the time of immunization and 48 h later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Materials:

PT and the purified PTS1 subunit were purchased from List Biological Laboratories (Campbell, CA).

Toxin detection and quantification by SPR:

Wild-type or mutant CHO cells were seeded into 6-well plates in order to achieve 80% confluence after an overnight incubation. The cells were then placed at 4°C for 30 min in 1 ml serum-free medium containing 1 µg of PT. …

Intoxication assay

Wild-type or mutant CHO cells were seeded into 6-well plates and grown overnight to 80% confluence. After a 30-min incubation at 4°C in 1 ml of Ham’s F-12 medium containing 250 ng PT, the cells were washed with PBS and returned to 37°C in serum- and toxin-free medium for 3 h. …

Confocal microscopy:

CHO cells seeded onto glass coverslips in a 24-well plate were incubated with 0.5 µg/ml of PT for 30 min at 4°C. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)