Citations

BLT Mouse Experiments:

For some experiments 1 mg/ml of LPS (List Biological Laboratories) was applied IVAG.

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

MPECs with floxed and Cre-deleted Map3k7 ( Wu et al., 2012 ) were maintained and cultured in DMEM/F12 (Gibco 11320) with fraction 5 BSA (Sigma), cholera toxin (List Biologicals 101B),…

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

EAE induction:

… Mice were intravenously injected with 100 µL Bordetella pertussis (BP) toxin (List Biological Laboratories, Inc., Campbell, CA, USA) at 0.2 µg per mouse on days 0 and 2 following sensitization. Mice developed a relapsing-remitting EAE clinical course. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE induction:

For EAE induction, mice were injected subcutaneously with 100µg MOG(3555) peptide…Two hundred nanograms of pertussis toxin (List Biological Laboratories, CA, USA) was injected intraperitoneally on days 0 and 2 after immunization. The clinical symptoms of EAE were scored…

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Pharmacological treatment:

Membrane-permeable caged cAMP…was applied to neuronal cultures 30 min before photolysis experiments….Unless otherwise stated, the following reagents were applied to bath media 30 min before microscopy or fixation:…tetanus neurotoxin (TeNT; 5 nm; List Biological Labs); …

• Product #190 – Tetanus Toxin from Clostridium tetani

…Phosphate buffered saline (PBS), 6 mg of peanut extract in PBS (n = 8) or cross-linked peanut proteins (n = 5) in PBS were co-administered with 15 µg of cholera toxin (List Biological Laboratories, Inc, Campbell, CA, USA) to mice by intragastric gavage on days 0, 1, 2, 7, 14 and …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

EAE:

The mice were injected ip with 200 ng pertussis toxin (List Biological Laboratories, Campbell, CA) on days 0 and 2 postimmunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

In Vitro T-Cell Homing Assay:

… pertussis toxin (List Biological) …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Reagents: 

 Recombinant full-length protective antigen (PA), lethal factor (LF), edema factor (EF) and 
 fluorescein-conjugated PA were purchased from List Biological Laboratories  (Campbell, CA, 
 U.S.A.).

PA-binding assay:

 To detect PA-binding ability of the cells, cells were washed twice with ice-cold PBS, scraped, and 
 resuspended in ice-cold PBS (4 x 106 cells / ml). Cell suspensions (each 25 l) were reacted with 1
 mg/ml of FITC-PA in 1.5 ml tubes and incubated at 4 C for 60 min in the dark.  The cells were
 then washed twice with 2 ml ice-cold PBS and re-suspended in 0.5 ml ice-cold PBS for
flowcytometric analysis as described above.

RESULTS:

 Sensitivity to ATX-mediated cytotoxicity has been confirmed in several cell types derived from 
 mice and humans.  Murine (Raw264.7 or J774.1) or human (THP1, U937 or K562) cells were 
 treated with 300 ng/ml of LF and with the indicated concentration of PA, and viabilities of the cells 
 were examined 24, 48 or 72 hr after the treatment (Fig. 1A).  In the case of murine cells, ATX
 treatment showed a cytotoxic effect in a PA dose-dependent manner at 24 hr after treatment. 
 Comparing with murine cells, human U937 and K562 cells had exhibited resistance to 
 ATX-mediated cytotoxicity in the presence of a high concentration (500 ng/ml) of PA.

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis 

Author did not specify which List Labs Anthrax Edema Factor was utilized.  List Labs provides Product #178A(Anthrax Edema Factor (EF), Recombinant from B. anthracis) and Product #173B (Anthrax Edema Factor (EF), Recombinant, Mutant S447N from B. anthracis).

EAE Induction:

Pertussis toxin (Cat# 180. 50 µg. List Biological Laboratories, Inc.) was diluted with sterile PBS to 100g/ml.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer